Strain identifier

BacDive ID: 158633

Type strain: Yes

Species: Saccharopolyspora deserti

Strain history: <- Wael Nabil Hozzein, King Saud Univ.

NCBI tax ID(s): 2081980 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 65356

BacDive-ID: 158633

keywords: 16S sequence, Bacteria, aerobe, halotolerant, spore-forming, Gram-positive

description: Saccharopolyspora deserti KCTC 39989 is an aerobe, halotolerant, spore-forming bacterium that builds substrate mycelia and was isolated from sand samples.

NCBI tax id

  • NCBI tax id: 2081980
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Wael Nabil Hozzein, King Saud Univ.

doi: 10.13145/bacdive158633.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Saccharopolyspora
  • species: Saccharopolyspora deserti
  • full scientific name: Saccharopolyspora deserti Yang et al. 2018

@ref: 65356

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudonocardiales

family: Pseudonocardiaceae

genus: Saccharopolyspora

species: Saccharopolyspora deserti

type strain: yes

Morphology

cell morphology

  • @ref: 65356
  • gram stain: positive

multicellular morphology

  • @ref: 65356
  • forms multicellular complex: yes
  • complex name: substrate mycelia
  • complex color: yellow
  • further description: Forms well-developed and extensively branched yellow substrate mycelia. Aerial mycelia form long chains of spores

Culture and growth conditions

culture medium

@refnamegrowth
65356ISP2yes
65356ISP3yes
65356ISP4yes
65356ISP5yes
65356Reasoner's 2A agar (R2A)yes
65356Czapek agaryes
65356ISP6yes
65356Nutrient agar (NA)yes
65356potato dextrose agaryes

culture temp

@refgrowthtypetemperaturerange
65356positivegrowth14-40
65356positiveoptimum28-37mesophilic
67771positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
65356positivegrowth5-9alkaliphile
65356positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65356aerobe
67771aerobe

spore formation

  • @ref: 65356
  • spore description: long chains of spores; spores are non-motile, smooth and oval in shape
  • type of spore: spore
  • spore formation: yes

halophily

@refhalophily levelsaltgrowthtested relationconcentration
65356halotolerantNaClpositivegrowth0-22 %(w/v)
65356NaClpositiveoptimum5-10 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6535616708adenine+nitrogen source
6535622599arabinose+carbon source
6535629016arginine+nitrogen source
6535617057cellobiose+carbon source
6535662968cellulose-hydrolysis
6535615356cysteine-nitrogen source
65356115156disodium fumarate-carbon source
6535628757fructose+carbon source
6535633984fucose+carbon source
6535628260galactose+carbon source
653565291gelatin-hydrolysis
6535617234glucose+carbon source
6535629987glutamate+nitrogen source
6535617754glycerol-carbon source
6535615428glycine-nitrogen source
6535627570histidine+nitrogen source
6535617368hypoxanthine+nitrogen source
6535617268myo-inositol-carbon source
6535617716lactose-carbon source
6535625094lysine+nitrogen source
6535617306maltose+carbon source
6535629864mannitol-carbon source
6535637684mannose+carbon source
6535616811methionine-nitrogen source
65356milk-assimilation
6535617632nitrate+reduction
6535628044phenylalanine+nitrogen source
6535626271proline+nitrogen source
6535616634raffinose-carbon source
6535626546rhamnose+carbon source
6535633942ribose+carbon source
6535617822serine-nitrogen source
6535650144sodium pyruvate-carbon source
6535663675sodium succinate-carbon source
6535630911sorbitol+carbon source
6535627922sorbose-carbon source
6535628017starch-hydrolysis
6535617992sucrose+carbon source
6535626986threonine+nitrogen source
6535627897tryptophan+nitrogen source
6535653424tween 20+hydrolysis
6535653423tween 40+hydrolysis
6535653425tween 60+hydrolysis
6535653426tween 80+hydrolysis
6535627266valine+nitrogen source
6535617151xylitol-carbon source
6535618222xylose+carbon source

metabolite production

  • @ref: 65356
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
65356catalase+1.11.1.6
65356urease+3.5.1.5
65356cytochrome oxidase-1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation proceduregeographic location
65356sand samplesSaudi ArabiaSAUAsia24.201238.2642R2Asupplemented with 5% (w/v) NaCl7 days37The samples were diluted 10-3-fold with sterile distilled water, and a 0.2 ml aliquot of the final suspension was plated on Reasoner's 2 agar
67771From desertSaudi ArabiaSAUAsiaRiyadh

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Sandy

Sequence information

16S sequences

  • @ref: 65356
  • description: 16S rRNA gene sequence
  • accession: MF480321
  • database: nuccore

GC content

  • @ref: 65356
  • GC-content: 69.9
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65356

culture collection no.: KCTC 39989, CPCC 204620, SYSU D8010

literature

  • topic: Phylogeny
  • Pubmed-ID: 29458666
  • title: Saccharopolyspora deserti sp. nov., a novel halotolerant actinobacterium isolated from a desert.
  • authors: Yang ZW, Salam N, Asem MD, Fang BZ, Lan L, Xiao M, Wadaan MAM, Hozzein WN, Li WJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002598
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saccharopolyspora/*classification/genetics/isolation & purification, *Salinity, Saudi Arabia, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65356Zi-Wen Yang, Nimaichand Salam, Mipeshwaree Devi Asem, Bao-Zhu Fang, Liu Lan, Min Xiao, Mohammed A. M. Wadaan, Wael N. Hozzein, Wen-Jun LiSaccharopolyspora deserti sp. nov., a novel halotolerant actinobacterium isolated from a desert10.1099/ijsem.0.002598IJSEM 68: 860-864 201829458666
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc