Strain identifier

BacDive ID: 158630

Type strain: Yes

Species: Bacillus manusensis

Strain Designation: Ma50-6

Strain history: <- Sun Qinglei, Institute of Oceanology, China

NCBI tax ID(s): 2078954 (species)

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General

@ref: 65353

BacDive-ID: 158630

keywords: 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, rod-shaped

description: Bacillus manusensis Ma50-6 is an aerobe, spore-forming, thermophilic bacterium that was isolated from hydrothermal sediments .

NCBI tax id

  • NCBI tax id: 2078954
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Sun Qinglei, Institute of Oceanology, China

doi: 10.13145/bacdive158630.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus manusensis
  • full scientific name: Bacillus manusensis Sun et al. 2018

@ref: 65353

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus manusensis

strain designation: Ma50-6

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shape
65353positive2-5 µm0.4-0.6 µmrod-shaped
67771rod-shaped
67771positive

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
65353positivegrowth45-70thermophilic
65353positiveoptimum50-55thermophilic
67771positivegrowth50thermophilic

culture pH

@refabilitytypepHPH range
65353positivegrowth7-10alkaliphile
65353positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

spore formation

@refspore descriptiontype of sporespore formation
65353Ellipsoidal endospores are formed centrally and usually cause the sporangia to swellendosporeyes
67771endosporeyes

halophily

@refsaltgrowthtested relationconcentration
65353NaClpositivegrowth0-8 %(w/v)
65353NaClpositiveoptimum3 %(w/v)

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6535327613amygdalin-builds acid from
6535318305arbutin-builds acid from
6535327689decanoate-assimilation
65353casein-hydrolysis
6535317057cellobiose-builds acid from
6535315963ribitol-builds acid from
6535317108D-arabinose-builds acid from
6535318333D-arabitol-builds acid from
6535315824D-fructose-builds acid from
6535328847D-fucose-builds acid from
6535312936D-galactose-builds acid from
6535317634D-glucose-builds acid from
6535317634D-glucose+assimilation
6535362318D-lyxose-builds acid from
6535317306maltose+assimilation
6535316899D-mannitol-builds acid from
6535316899D-mannitol+assimilation
6535316024D-mannose-assimilation
6535316024D-mannose-builds acid from
6535316988D-ribose-builds acid from
6535317924D-sorbitol-builds acid from
6535316443D-tagatose-builds acid from
6535365327D-xylose-builds acid from
6535316813galactitol-builds acid from
6535317113erythritol-builds acid from
653534853esculin-builds acid from
653534853esculin+hydrolysis
65353esculin ferric citrate+builds acid from
6535328260galactose-builds acid from
6535328066gentiobiose-builds acid from
6535317234glucose+builds acid from
6535317754glycerol-builds acid from
6535328087glycogen-builds acid from
6535328087glycogen+builds acid from
6535317268myo-inositol-builds acid from
6535315443inulin-builds acid from
6535330849L-arabinose-assimilation
6535330849L-arabinose-builds acid from
6535318403L-arabitol-builds acid from
6535318287L-fucose-builds acid from
6535362345L-rhamnose-builds acid from
6535317266L-sorbose-builds acid from
6535365328L-xylose-builds acid from
6535317716lactose-builds acid from
6535325097lyxose-builds acid from
6535317306maltose-builds acid from
6535317306maltose+builds acid from
6535329864mannitol+builds acid from
6535337684mannose-builds acid from
653536731melezitose-builds acid from
6535328053melibiose-builds acid from
65353320061methyl alpha-D-glucopyranoside-builds acid from
6535343943methyl alpha-D-mannoside-builds acid from
6535374863methyl beta-D-xylopyranoside-builds acid from
65353506227N-acetylglucosamine-builds acid from
6535317632nitrate-reduction
6535364681phenyl beta-D-glucuronide-hydrolysis
65353potassium 2-dehydro-D-gluconate-builds acid from
65353potassium 5-dehydro-D-gluconate+builds acid from
6535332032potassium gluconate-builds acid from
6535332032potassium gluconate+assimilation
6535316634raffinose-builds acid from
6535317814salicin-builds acid from
6535330911sorbitol-builds acid from
6535328017starch-builds acid from
6535328017starch-hydrolysis
6535317992sucrose-builds acid from
6535317992sucrose+builds acid from
6535327082trehalose-builds acid from
6535327082trehalose+builds acid from
6535327897tryptophan-energy source
6535332528turanose-builds acid from
6535353424tween 20+hydrolysis
6535353423tween 40-hydrolysis
6535353425tween 60-hydrolysis
6535353426tween 80-hydrolysis
6535317151xylitol-builds acid from
6535318222xylose-builds acid from

metabolite production

  • @ref: 65353
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 65353
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
65353tryptophan deaminase-4.1.99.1
65353catalase+1.11.1.6
65353esterase (C 4)+
65353esterase Lipase (C 8)+
65353leucine arylamidase+3.4.11.1
65353chymotrypsin+3.4.4.5
65353alpha-glucosidase+3.2.1.20
65353lipase (C 14)-
65353valine arylamidase-
65353alpha-galactosidase-3.2.1.22
65353beta-galactosidase-3.2.1.23
65353N-acetyl-beta-glucosaminidase-3.2.1.52
65353alpha-mannosidase-3.2.1.24
65353alpha-fucosidase-3.2.1.51
65353cytochrome oxidase-1.9.3.1
65353urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountrylatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
65353hydrothermal sediments (depth of 1851m)2015-06Manus BasinPacific Ocean3.73151.68marine agar(l-1): peptone, 5.0 g; yeast extract, 1.0 g; Fe(III) citrate, 0.1 g; NaCl, 19.45 g; MgCl2, 5.98 g; Na2SO4, 3.24 g; CaCl2, 1.8 g; KCl, 0.55 g; Na2CO3, 0.16 g; KBr, 0.08 g; SrCl2, 0.034 g; H3BO3, 0.022 g; Na2SiO3, 0.004 g; NaF, 0.0024 g; (NH4)NO3, 0.0016 g; Na2HPO4, 0.008 g; agar 15.0 g.3 days501.0 g of the sediment sample was suspended with 3 mm sterile seawater, and a 100 µl suspension was spread on marine agar plates. Incubation for 3 days at 50°C under aerobic conditions.
67771From hydrothermal marine sediments, depth 1851mManus Basin, western Pacific OceanPacific Ocean-3.73423151.677

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Hydrothermal vent
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_75172.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1802;97_27076;98_52568;99_75172&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: MF582328
  • Sequence Identity:
  • Total samples: 76
  • soil counts: 49
  • aquatic counts: 21
  • animal counts: 4
  • plant counts: 2

Sequence information

16S sequences

  • @ref: 65353
  • description: 16S rRNA gene sequence
  • accession: MF582328
  • database: nuccore

GC content

@refGC-contentmethod
6535347.6high performance liquid chromatography (HPLC)
6777147.6

External links

@ref: 65353

culture collection no.: KCTC 33882, CCTCC AB 2017019

literature

  • topic: Phylogeny
  • Pubmed-ID: 29458542
  • title: Description of Bacillus kexueae sp. nov. and Bacillus manusensis sp. nov., isolated from hydrothermal sediments.
  • authors: Sun QL, Yu C, Luan ZD, Lian C, Hu YH, Sun L
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002594
  • year: 2018
  • mesh: Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Hydrothermal Vents/*microbiology, Nucleic Acid Hybridization, Pacific Ocean, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65353Qing-lei Sun, Chao Yu, Zhen-dong Luan, Chao Lian, Yong-hua Hu, Li SunDescription of Bacillus kexueae sp. nov. and Bacillus manusensis sp. nov., isolated from hydrothermal sediments10.1099/ijsem.0.002594IJSEM 68: 829-834 201829458542
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/