Strain identifier

BacDive ID: 158624

Type strain: Yes

Species: Euzebyella algicola

Strain history: Y. M. Kwon; Natl. Mar. Biodivers. Inst. of Korea, South Korea; MEBiC12267.

NCBI tax ID(s): 2083203 (species)

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General

@ref: 65349

BacDive-ID: 158624

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped

description: Euzebyella algicola KCCM 43264 is an aerobe, Gram-negative, motile bacterium that was isolated from sample of the green algae Ulva species.

NCBI tax id

  • NCBI tax id: 2083203
  • Matching level: species

strain history

  • @ref: 67770
  • history: Y. M. Kwon; Natl. Mar. Biodivers. Inst. of Korea, South Korea; MEBiC12267.

doi: 10.13145/bacdive158624.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Euzebyella
  • species: Euzebyella algicola
  • full scientific name: Euzebyella algicola Kwon et al. 2018

@ref: 65349

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Euzebyella

species: Euzebyella algicola

type strain: yes

Morphology

cell morphology

  • @ref: 65349
  • gram stain: negative
  • cell length: 1.2-3.4 µm
  • cell width: 0.3-0.4 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: gliding

colony morphology

  • @ref: 65349
  • colony color: yellow
  • medium used: marine agar 2216

Culture and growth conditions

culture medium

  • @ref: 65349
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65349positivegrowth10-37
65349positiveoptimum30-33mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
65349positivegrowth6-9.5alkaliphile
65349positiveoptimum8-8.5

Physiology and metabolism

oxygen tolerance

  • @ref: 65349
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
65349NaClpositivegrowth0.5-9 %(w/v)
65349NaClpositiveoptimum2.5-3.5 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
65349645522-hydroxybutyrate-respiration
65349167632-oxobutanoate-respiration
65349309162-oxoglutarate-respiration
65349286442-oxopentanoate-respiration
65349620642,3-butanediol-respiration
65349370543-hydroxybutyrate-respiration
65349182404-hydroxy-L-proline+respiration
65349167244-hydroxybutyrate-respiration
65349181014-hydroxyphenylacetic acid-respiration
6534930089acetate-respiration
6534917128adipate-assimilation
6534915963ribitol-respiration
6534940585alpha-cyclodextrin+respiration
6534917925alpha-D-glucose+respiration
6534936219alpha-lactose+respiration
6534927613amygdalin+builds acid from
6534922599arabinose+assimilation
6534918305arbutin+builds acid from
6534973706bromosuccinate-respiration
6534929108calcium(2+)+required for growth
6534927689decanoate-assimilation
65349casein-hydrolysis
6534917057cellobiose+builds acid from
6534916383cis-aconitate-respiration
6534916947citrate-assimilation
6534916947citrate-respiration
6534915963ribitol-builds acid from
6534915570D-alanine-respiration
6534917108D-arabinose+builds acid from
6534918333D-arabitol-builds acid from
6534918333D-arabitol-respiration
6534917057cellobiose+respiration
6534915824D-fructose+builds acid from
6534915824D-fructose+respiration
6534928847D-fucose+builds acid from
6534915895D-galactonic acid lactone-respiration
6534912936D-galactose+builds acid from
6534912936D-galactose+respiration
6534918024D-galacturonic acid+respiration
6534930612D-glucarate-respiration
653498391D-gluconate-respiration
6534917784D-glucosaminic acid-respiration
6534917634D-glucose+builds acid from
6534914314D-glucose 6-phosphate-respiration
6534915748D-glucuronate+respiration
6534962318D-lyxose+builds acid from
6534916899D-mannitol-builds acid from
6534916899D-mannitol-respiration
6534916024D-mannose+builds acid from
6534916024D-mannose+respiration
6534927605D-psicose-respiration
6534916988D-ribose-builds acid from
6534916523D-serine-respiration
6534917924D-sorbitol-builds acid from
6534917924D-sorbitol-respiration
6534916443D-tagatose+builds acid from
6534965327D-xylose+builds acid from
6534923652dextrin+respiration
6534917126DL-carnitine-respiration
6534924996lactate-respiration
6534916813galactitol-builds acid from
6534917113erythritol-builds acid from
6534917113erythritol-respiration
653494853esculin+hydrolysis
65349esculin ferric citrate+builds acid from
6534916000ethanolamine-respiration
6534915740formate-respiration
6534916865gamma-aminobutyric acid+respiration
653495291gelatin-hydrolysis
6534928066gentiobiose+builds acid from
6534928066gentiobiose+respiration
6534924265gluconate+assimilation
6534917234glucose-fermentation
6534917234glucose+assimilation
6534929042glucose 1-phosphate+respiration
6534932323glucuronamide-respiration
6534917754glycerol-builds acid from
6534917754glycerol-respiration
6534914336glycerol 1-phosphate-respiration
6534928087glycogen-builds acid from
6534928087glycogen+respiration
6534973804glycyl L-aspartic acid+respiration
6534917596inosine-respiration
6534917268myo-inositol-builds acid from
6534915443inulin-builds acid from
6534917240itaconate-respiration
6534921217L-alaninamide-respiration
6534916977L-alanine-respiration
6534973786L-alanylglycine-respiration
6534930849L-arabinose-respiration
6534930849L-arabinose+builds acid from
6534918403L-arabitol-builds acid from
6534917196L-asparagine+respiration
6534929991L-aspartate+respiration
6534918287L-fucose-respiration
6534918287L-fucose+builds acid from
6534929985L-glutamate+respiration
6534915971L-histidine-respiration
6534915603L-leucine-respiration
6534915729L-ornithine+respiration
6534917295L-phenylalanine-respiration
6534917203L-proline-respiration
6534918183L-pyroglutamic acid-respiration
6534962345L-rhamnose+builds acid from
6534962345L-rhamnose+respiration
6534917115L-serine-respiration
6534917266L-sorbose-builds acid from
6534916857L-threonine-respiration
6534965328L-xylose-builds acid from
6534917716lactose+builds acid from
653496359lactulose+respiration
6534918420magnesium(2+)+required for growth
6534925115malate-assimilation
6534915792malonate-respiration
6534917306maltose+assimilation
6534917306maltose+builds acid from
6534917306maltose+respiration
6534929864mannitol-assimilation
6534937684mannose+assimilation
653496731melezitose-builds acid from
6534928053melibiose+builds acid from
6534928053melibiose+respiration
65349320061methyl alpha-D-glucopyranoside+builds acid from
6534943943methyl alpha-D-mannoside+builds acid from
65349320055methyl beta-D-glucopyranoside+respiration
6534974863methyl beta-D-xylopyranoside+builds acid from
6534951850methyl pyruvate-respiration
6534975146monomethyl succinate-respiration
6534917268myo-inositol-respiration
6534928037N-acetylgalactosamine-respiration
65349506227N-acetylglucosamine+assimilation
65349506227N-acetylglucosamine+builds acid from
65349506227N-acetylglucosamine+respiration
6534973784glycyl-l-glutamate+respiration
6534917632nitrate+reduction
6534918401phenylacetate-assimilation
6534950048phenylethylamine-respiration
65349potassium 2-dehydro-D-gluconate-builds acid from
65349potassium 5-dehydro-D-gluconate-builds acid from
6534932032potassium gluconate-builds acid from
6534917272propionate-respiration
6534917148putrescine-respiration
6534926490quinate-respiration
6534916634raffinose+builds acid from
6534916634raffinose+respiration
6534917814salicin+builds acid from
6534941865sebacic acid-respiration
6534928017starch+builds acid from
6534928017starch+hydrolysis
65349143136succinamate-respiration
6534930031succinate+respiration
6534917992sucrose+builds acid from
6534917992sucrose+respiration
6534917748thymidine-respiration
6534927082trehalose+builds acid from
6534927082trehalose+respiration
6534932528turanose+builds acid from
6534932528turanose+respiration
6534953423tween 40-hydrolysis
6534953423tween 40-respiration
6534953425tween 60-hydrolysis
6534953426tween 80-hydrolysis
6534953426tween 80-respiration
6534916704uridine-respiration
6534927248urocanic acid-respiration
6534917151xylitol-builds acid from
6534917151xylitol-respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6534928077rifampicinyesyes5 µg (disc)
6534928971ampicillinyesyes10 µg (disc)
6534948923erythromycinyesyes15 µg (disc)
6534917698chloramphenicolyesyes30 µg (disc)
65349100147nalidixic acidyesyes30 µg (disc)
6534928001vancomycinyesyes30 µg (disc)
6534917833gentamicinyesyes10 µg (disc)
6534917334penicillinyesyes10 Unit (disc)
6534917076streptomycinyesyes10 µg (disc)
653496104kanamycinyesyes30 µg (disc)
6534927902tetracyclineyesyes30 µg (disc)

metabolite production

  • @ref: 65349
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65349cytochrome oxidase+1.9.3.1
65349catalase+1.11.1.6
65349DNase+
65349alkaline phosphatase+3.1.3.1
65349esterase (C 4)+
65349esterase Lipase (C 8)+
65349leucine arylamidase+3.4.11.1
65349valine arylamidase+
65349acid phosphatase+3.1.3.2
65349naphthol-AS-BI-phosphohydrolase+
65349alpha-glucosidase+3.2.1.20
65349N-acetyl-beta-glucosaminidase+3.2.1.52
65349alpha-mannosidase+3.2.1.24
65349cystine arylamidase+3.4.11.3
65349trypsin+3.4.21.4
65349alpha-galactosidase+3.2.1.22
65349arginine dihydrolase-3.5.3.6
65349urease-3.5.1.5
65349lipase (C 14)-
65349alpha-chymotrypsin-3.4.21.1
65349beta-glucosidase-3.2.1.21
65349beta-glucuronidase-3.2.1.31
65349alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65349C16:01.6
    65349C15:0 iso33.8
    65349C15:1 iso G15
    65349C15:1ω6c1.3
    65349C16:0 3OH2.2
    65349C15:0 iso 3OH6.4
    65349C17:0 iso 3OH23.5
    65349C16:1ω7c and/or C16:1ω6c5.2
    65349C16:0 10-methyl and/or iso-C17:1ω9c3.2
  • type of FA analysis: whole cell analysis
  • incubation medium: marine agar 2216
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.3
  • library/peak naming table: RTSBA6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedurehost species
65349sample of the green algae Ulva species2015-10-06coastal region of Jeju IslandRepublic of KoreaKORAsia33.5075126.896marine agar 22165 days25a small piece of algal frond was homogenized, diluted with sterile seawater and spread onto marine agar 2216 . Inoculated plates were incubated at 25°C for 5 days.
67770Green algae Ulva species collected from a coastal region of Jeju IslandRepublic of KoreaKORAsiaUlva

isolation source categories

  • Cat1: #Host
  • Cat2: #Algae
  • Cat3: #Green algae

taxonmaps

  • @ref: 69479
  • File name: preview.99_55571.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_141;96_24772;97_31015;98_39691;99_55571&stattab=map
  • Last taxonomy: Euzebyella algicola subclade
  • 16S sequence: MF416137
  • Sequence Identity:
  • Total samples: 245
  • soil counts: 6
  • aquatic counts: 222
  • animal counts: 17

Sequence information

16S sequences

  • @ref: 65349
  • description: 16S rRNA gene sequence
  • accession: MF416137
  • database: nuccore

GC content

  • @ref: 65349
  • GC-content: 40.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65349

culture collection no.: KCCM 43264, JCM 32170, MEBiC 12267

literature

  • topic: Phylogeny
  • Pubmed-ID: 29458477
  • title: Euzebyella algicola sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from green algae.
  • authors: Kwon YM, Kim KW, Kim JYH, Choi TY, Yang SH, Oh CH, Kwon KK, Kim SJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002581
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, Chlorophyta/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65349Yong Min Kwon, Kyung Woo Kim, Jaoon Young Hwan Kim, Tae-Young Choi, Sung-Hyun Yang, Chul Hong Oh, Kae Kyoung Kwon, Sang-Jin KimEuzebyella algicola sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from green algae10.1099/ijsem.0.002581IJSEM 68: 764-768 201829458477
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/