Strain identifier

BacDive ID: 158600

Type strain: Yes

Species: Halorubrum aethiopicum

Strain Designation: SAH-A6

Strain history: S. W. Roh; Div. of Convergence Anal., Korea Basic Sci. Inst. (KBSI), South Korea; SAH-A6.

NCBI tax ID(s): 1758255 (species)

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General

@ref: 65316

BacDive-ID: 158600

keywords: genome sequence, 16S sequence, Archaea, Gram-negative, ovoid-shaped, colony-forming

description: Halorubrum aethiopicum SAH-A6 is a Gram-negative, ovoid-shaped archaeon that forms circular colonies and was isolated from commercial rock salt from Lake Afdera.

NCBI tax id

  • NCBI tax id: 1758255
  • Matching level: species

strain history

  • @ref: 67770
  • history: S. W. Roh; Div. of Convergence Anal., Korea Basic Sci. Inst. (KBSI), South Korea; SAH-A6.

doi: 10.13145/bacdive158600.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Haloferacales
  • family: Halorubraceae
  • genus: Halorubrum
  • species: Halorubrum aethiopicum
  • full scientific name: Halorubrum aethiopicum Gibtan et al. 2018

@ref: 65316

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Haloferacales

family: Halorubraceae

genus: Halorubrum

species: Halorubrum aethiopicum

strain designation: SAH-A6

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapecell width
65316negative0.5-0.8 µmovoid-shaped
653161-1.5 µmrod-shaped0.4-0.6 µm

colony morphology

  • @ref: 65316
  • colony color: red
  • colony shape: circular

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
65316positivegrowth30-55
65316positiveoptimum37-45
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
65316positivegrowth6-9alkaliphile
65316positiveoptimum7

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
65316NaClpositivegrowth15-30 %
65316NaClpositiveoptimum20-25 %

observation

  • @ref: 65316
  • observation: Does not require Mg2+ for growth.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6531630089acetate-assimilation
65316casein-hydrolysis
6531616947citrate-assimilation
6531624996lactate-assimilation
6531616991dna-hydrolysis
6531629806fumarate-assimilation
653165291gelatin-hydrolysis
6531617234glucose-assimilation
6531617754glycerol-assimilation
6531616977L-alanine-builds acid from
6531616977L-alanine+assimilation
6531616467L-arginine-builds acid from
6531616467L-arginine+assimilation
6531629991L-aspartate-assimilation
6531629985L-glutamate-builds acid from
6531629985L-glutamate+assimilation
6531618019L-lysine-assimilation
6531615589L-malate-assimilation
6531617716lactose-assimilation
6531617306maltose-assimilation
6531629864mannitol-assimilation
6531617632nitrate+reduction
6531615361pyruvate-builds acid from
6531615361pyruvate+assimilation
6531630911sorbitol-assimilation
6531628017starch-assimilation
6531628017starch-hydrolysis
6531630031succinate-builds acid from
6531630031succinate+assimilation
6531617992sucrose-assimilation
6531653424tween 20-hydrolysis
6531653423tween 40-hydrolysis
6531653426tween 80-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6531628077rifampicinyesyes30 µg (disc)
6531628971ampicillinyesyes10 µg (disc)
6531617833gentamicinyesyes10 µg (disc)
6531617076streptomycinyesyes10 µg (disc)
6531648923erythromycinyesyes15 µg (disc)
6531627641cycloheximideyesyes30 µg (disc)
653166104kanamycinyesyes30 µg (disc)
65316100147nalidixic acidyesyes30 µg (disc)
6531627902tetracyclineyesyes30 µg (disc)
6531628001vancomycinyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6531616136hydrogen sulfideno
6531635581indoleno

enzymes

@refvalueactivityec
65316catalase+1.11.1.6
65316cytochrome oxidase-1.9.3.1
65316urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
65316commercial rock salt from Lake AfderaLake AfderaEthiopiaETHAfrica13.283340.9166modified growth mediumNaCl concentration of 20 % (w/v)2-4 weeks50
67770Commercial rock salt in Ethiopia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Geologic
#Condition#Saline

Sequence information

16S sequences

  • @ref: 65316
  • description: 16S rRNA gene sequence
  • accession: KX150521
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Halorubrum aethiopicum SAH-A62684622567draftimg1758255
67770Halorubrum aethiopicum SAH-A6GCA_001542905contigncbi1758255

GC content

  • @ref: 67770
  • GC-content: 68
  • method: genome sequence analysis

External links

@ref: 65316

culture collection no.: KCCM 43215, JCM 31519

literature

  • topic: Phylogeny
  • Pubmed-ID: 29227219
  • title: Halorubrum aethiopicum sp. nov., an extremely halophilic archaeon isolated from commercial rock salt.
  • authors: Gibtan A, Song HS, Kim JY, Kim YB, Park N, Park K, Lee SJ, Kwon J, Roh SW, Lee HS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002525
  • year: 2017
  • mesh: Base Composition, DNA, Archaeal/genetics, Ethiopia, Halorubrum/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65316Ashagrie Gibtan, Hye Seon Song, Joon Yong Kim, Yeon Bee Kim, Nakyeong Park, Kyounghee Park, Sang-Jae Lee, Joseph Kwon, Seong Woon Roh, Han-Seung LeeHalorubrum aethiopicum sp. nov., an extremely halophilic archaeon isolated from commercial rock salt10.1099/ijsem.0.002525IJSEM 68: 416-422 201829227219
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/