Strain identifier

BacDive ID: 158591

Type strain: Yes

Species: Thalassotalea atypica

Strain Designation: RZG4-3-1

Strain history: <- Yan Wang, Xin Xiang Medical Univ.

NCBI tax ID(s): 2054316 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9 (current version):
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 9 (current version)

General

@ref: 65305

BacDive-ID: 158591

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Thalassotalea atypica RZG4-3-1 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from surface seawater.

NCBI tax id

  • NCBI tax id: 2054316
  • Matching level: species

strain history

@refhistory
67770Y. Wang; Sch. of Life Sci. & Technol., Xinxiang Med. Univ., China; RZG4-3-1.
67771<- Yan Wang, Xin Xiang Medical Univ.

doi: 10.13145/bacdive158591.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Colwelliaceae
  • genus: Thalassotalea
  • species: Thalassotalea atypica
  • full scientific name: Thalassotalea atypica Wang et al. 2018

@ref: 65305

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Colwelliaceae

genus: Thalassotalea

species: Thalassotalea atypica

strain designation: RZG4-3-1

type strain: yes

Morphology

cell morphology

  • @ref: 65305
  • gram stain: negative
  • cell length: 1.5-2.5 µm
  • cell width: 0.5-1 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65305
  • colony size: 1-2 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 2-3 days
  • medium used: marine agar 2216

pigmentation

  • @ref: 65305
  • production: no
  • name: flexirubin-type pigments

Culture and growth conditions

culture medium

  • @ref: 65305
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperature
65305positivegrowth4-30
65305positiveoptimum24-28
67770positivegrowth28
67771positivegrowth28

culture pH

  • @ref: 65305
  • ability: positive
  • type: growth
  • pH: 6-9
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65305aerobe
67771aerobe

halophily

@refsaltgrowthtested relationconcentration
65305NaClpositivegrowth1-4 %(w/v)
65305NaClpositiveoptimum2 %(w/v)

observation

  • @ref: 65305
  • observation: NaCl alone does not support growth; requires seawater or artificial seawater for growth.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
653053557154-nitrophenyl beta-D-galactopyranoside-builds acid from
6530530089acetate-carbon source
653052509agar-hydrolysis
6530558187alginate+carbon source
6530558187alginate+hydrolysis
6530527613amygdalin+builds acid from
6530518305arbutin-builds acid from
65305casein-hydrolysis
6530517057cellobiose-builds acid from
6530517057cellobiose-carbon source
6530517029chitin-hydrolysis
6530516947citrate-carbon source
6530515963ribitol-builds acid from
6530517108D-arabinose-builds acid from
6530518333D-arabitol-builds acid from
6530515824D-fructose-builds acid from
6530528847D-fucose-builds acid from
6530512936D-galactose-builds acid from
6530512936D-galactose-carbon source
6530517634D-glucose-builds acid from
6530517634D-glucose-carbon source
6530562318D-lyxose-builds acid from
6530516899D-mannitol-builds acid from
6530516024D-mannose-builds acid from
6530516024D-mannose-carbon source
6530516988D-ribose-builds acid from
6530517924D-sorbitol-builds acid from
6530516443D-tagatose-builds acid from
6530565327D-xylose-builds acid from
6530565327D-xylose-carbon source
6530516449dl-alanine-carbon source
6530516991dna-hydrolysis
6530516813galactitol-builds acid from
6530517113erythritol-builds acid from
653054853esculin+hydrolysis
65305esculin ferric citrate-builds acid from
6530528757fructose-carbon source
653055291gelatin-hydrolysis
6530528066gentiobiose-builds acid from
6530517754glycerol-builds acid from
6530517754glycerol-carbon source
6530528087glycogen-builds acid from
6530517268myo-inositol-builds acid from
6530517268myo-inositol-carbon source
6530515443inulin-builds acid from
6530530849L-arabinose-builds acid from
6530518403L-arabitol-builds acid from
6530518287L-fucose-builds acid from
6530529985L-glutamate+carbon source
6530515971L-histidine-carbon source
6530515603L-leucine+carbon source
6530517295L-phenylalanine-carbon source
6530517203L-proline+carbon source
6530562345L-rhamnose-builds acid from
6530517266L-sorbose-builds acid from
6530516857L-threonine+carbon source
6530516828L-tryptophan-carbon source
6530516414L-valine+carbon source
6530565328L-xylose-builds acid from
6530517716lactose-builds acid from
6530517716lactose-carbon source
6530525094lysine-carbon source
6530517306maltose-builds acid from
6530517306maltose-carbon source
653056731melezitose-builds acid from
6530528053melibiose-builds acid from
65305320061methyl alpha-D-glucopyranoside-builds acid from
6530543943methyl alpha-D-mannoside-builds acid from
6530574863methyl beta-D-xylopyranoside-builds acid from
65305506227N-acetylglucosamine-builds acid from
6530517632nitrate+reduction
65305potassium 2-dehydro-D-gluconate-builds acid from
65305potassium 5-dehydro-D-gluconate-builds acid from
6530532032potassium gluconate-builds acid from
6530516634raffinose-builds acid from
6530517814salicin-builds acid from
6530517822serine-carbon source
6530528017starch-builds acid from
6530528017starch-hydrolysis
6530517992sucrose-builds acid from
6530517992sucrose-carbon source
6530527082trehalose-builds acid from
6530532528turanose-builds acid from
6530553424tween 20+carbon source
6530553424tween 20+hydrolysis
6530553423tween 40+carbon source
6530553423tween 40+hydrolysis
6530553425tween 60+carbon source
6530553425tween 60+hydrolysis
6530553426tween 80+carbon source
6530553426tween 80+hydrolysis
6530516199urea-hydrolysis
6530517151xylitol-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
65305100241ciprofloxacinyesyes5 µg (disc)
6530517334penicillinyesyes10 µg (disc)
6530528971ampicillinyesyes10 µg (disc)
6530548923erythromycinyesyes15 µg (disc)
65305474053cefazolinyesyes20 µg (disc)
653052637amikacinyesyes30 µg (disc)
6530528001vancomycinyesyes30 µg (disc)
6530517698chloramphenicolyesyes30 µg (disc)
653053493cefoperazoneyesyes75 µg (disc)
653053393carbenicillinyesyes100 µg (disc)
653058232piperacillinyesyes100 µg (disc)
653058309polymyxin byesyes300 µg (disc)
653057809oxacillinyesyes1 µg (disc)
653053745clindamycinyesyes2 µg (disc)
653057731ofloxacinyesyes5 µg (disc)
6530517833gentamicinyesyes10 µg (disc)
65305100246norfloxacinyesyes10 µg (disc)
6530550845doxycyclineyesyes30 µg (disc)
653053508ceftazidimeyesyes30 µg (disc)
6530527902tetracyclineyesyes30 µg (disc)
653057507neomycinyesyes30 µg (disc)
653056104kanamycinyesyes30 µg (disc)
6530531845midecamycinyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6530516136hydrogen sulfideno
6530535581indoleno

enzymes

@refvalueactivityec
65305cytochrome oxidase+1.9.3.1
65305catalase-1.11.1.6
65305alkaline phosphatase+3.1.3.1
65305esterase (C 4)+
65305esterase Lipase (C 8)+
65305leucine arylamidase+3.4.11.1
65305valine arylamidase+
65305acid phosphatase+3.1.3.2
65305naphthol-AS-BI-phosphohydrolase+
65305lipase (C 14)-
65305N-acetyl-beta-glucosaminidase-3.2.1.52
65305cystine arylamidase-3.4.11.3
65305trypsin-3.4.21.4
65305alpha-chymotrypsin-3.4.21.1
65305alpha-galactosidase-3.2.1.22
65305beta-galactosidase-3.2.1.23
65305beta-glucuronidase-3.2.1.31
65305alpha-glucosidase-3.2.1.20
65305beta-glucosidase-3.2.1.21
65305alpha-mannosidase-3.2.1.24
65305alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65305C12:01.7
    65305C14:05.1
    65305C16:018.5
    65305C17:01.2
    65305C18:01.4
    65305C15:1ω8c4.9
    65305C16:1ω9c4.9
    65305C17:1ω8c9.5
    65305C18:1ω7c3.3
    65305C10:0 3OH1.7
    65305C11:0 3OH2.1
    65305C12:0 3OH3.9
    65305C16:1ω7c and/or C16:1ω6c36
  • type of FA analysis: whole cell analysis
  • incubation medium: marine agar 2216
  • incubation temperature: 28
  • incubation time: 1.5
  • software version: Sherlock 6.1
  • library/peak naming table: RTSBA6 6.10
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture temperatureisolation procedure
65305surface seawater2014-11coastal region of the Yellow Sea, RizhaoChinaCHNAsia35.517119.625marine agar 221625Seawater samples were spread on marine agar 2216 and incubated at 25°C. Strain RZG4-3-1T was purified by streaking three times on MA.
67770Coastal seawater of the Yellow SeaRizhaoChinaCHNAsia
67771From marine seawaterSeawater of Yellow sea of ChinaChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Surface water

taxonmaps

  • @ref: 69479
  • File name: preview.99_14968.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_82;96_7688;97_9235;98_11328;99_14968&stattab=map
  • Last taxonomy: Thalassotalea atypica subclade
  • 16S sequence: KY014437
  • Sequence Identity:
  • Total samples: 380
  • soil counts: 4
  • aquatic counts: 372
  • animal counts: 2
  • plant counts: 2

Sequence information

16S sequences

  • @ref: 65305
  • description: Thalassotalea atypica strain RZG4-3-1 16S ribosomal RNA gene, partial sequence
  • accession: KY014437
  • database: nuccore

GC content

  • @ref: 65305
  • GC-content: 40.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65305

culture collection no.: JCM 31894, KCTC 52745, MCCC 1K03276

literature

  • topic: Phylogeny
  • Pubmed-ID: 29143725
  • title: Thalassotalea atypica sp. nov., isolated from seawater, and emended description of Thalassotalea eurytherma.
  • authors: Wang Y, Liu T, Ming H, Sun P, Cao C, Guo M, Du J, Zhou C, Zhu W
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002495
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, Phosphatidylethanolamines/chemistry, Phosphatidylglycerols/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65305Yan Wang, Tingwei Liu, Hong Ming, Pengyang Sun, Chenxi Cao, Menglong Guo, Junran Du, Chenyan Zhou, Wuling ZhuThalassotalea atypica sp. nov., isolated from seawater, and emended description of Thalassotalea eurytherma10.1099/ijsem.0.002495IJSEM 68: 271-276 201829143725
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/