General

@ref: 65304

BacDive-ID: 158590

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Rheinheimera marina TP462 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from from the surface of a black stone with brittle stars at a depth of 1103 m.

NCBI tax id

• NCBI tax id: 1774958
• Matching level: species

doi: 10.13145/bacdive158590.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/pseudomonadota
• domain: Bacteria
• phylum: Pseudomonadota
• class: Gammaproteobacteria
• order: Chromatiales
• family: Chromatiaceae
• genus: Rheinheimera
• species: Rheinheimera marina
• full scientific name: Rheinheimera marina Wang et al. 2018

@ref: 65304

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Chromatiaceae

genus: Rheinheimera

species: Rheinheimera marina

strain designation: TP462

type strain: yes

Morphology

cell morphology

• @ref: 65304
• gram stain: negative
• cell length: 1.1-2.4 µm
• cell width: 0.5-0.7 µm
• cell shape: rod-shaped
• motility: yes
• flagellum arrangement: monotrichous, polar

colony morphology

• @ref: 65304
• colony color: white
• colony shape: circular
• medium used: marine agar 2216

Culture and growth conditions

culture medium

• @ref: 65304
• name: Marine agar (MA)
• growth: yes

culture temp

culture pH

Physiology and metabolism

oxygen tolerance

• @ref: 65304
• oxygen tolerance: aerobe

halophily

metabolite utilization

metabolite production

metabolite tests

enzymes

fatty acid profile

• fatty acids

  @ref	fatty acid	percentage
  65304	C9:0	1.3
  65304	C10:0	2.5
  65304	C10:0 3OH	0.5
  65304	C11:0	1
  65304	C11:0 3OH	5.6
  65304	C12:0	0.6
  65304	C12:0 iso 3OH	1
  65304	C12:0 3OH	8.3
  65304	C13:0	0.5
  65304	C14:0	0.8
  65304	C14:0 iso	0.4
  65304	C15:0	4.8
  65304	C15:1ω6c	0.6
  65304	C15:1ω8c	3.9
  65304	C16:0	11.9
  65304	C16:0 iso	1.9
  65304	C16:1ω9c	0.9
  65304	C17:0	4.7
  65304	C17:1ω6c	1.4
  65304	C17:1ω8c	17
  65304	C18:0	0.3
  65304	C18:1ω7c	6.7
  65304	C13:0 3-OH and/or iso-C15:1 H	1.5
  65304	C16:1 iso I and/or C14:0 iso 3OH	0.3
  65304	C15:0 iso 2OH and/or C16:1ω7c	18.3

• type of FA analysis: whole cell analysis
• incubation medium: marine agar 2216
• incubation temperature: 25
• incubation time: 3
• software version: Sherlock 6.1
• library/peak naming table: TSBA40
• system: MIS MIDI

Isolation, sampling and environmental information

isolation

• @ref: 65304
• sample type: from the surface of a black stone with brittle stars at a depth of 1103 m
• sampling date: 2014-12
• geographic location: seamount (tentatively named the Yap-3 seamount) near the Yap Trench in the tropical western Pacific
• country: Pacific Ocean
• latitude: 8.75
• longitude: 137.683
• enrichment culture: marine agar 2216
• enrichment culture duration: 2 weeks
• enrichment culture temperature: 20
• isolation procedure: The sample was washed with sterile saline solution (0.8 %) immediately, and appropriate dilutions were then plated on marine agar 2216 at 20°C for 2 weeks.

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_72123.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_43;96_2516;97_38869;98_50543;99_72123&stattab=map
• Last taxonomy: Pararheinheimera
• 16S sequence: KT900247
• Sequence Identity:
• Total samples: 103
• soil counts: 2
• aquatic counts: 61
• animal counts: 39
• plant counts: 1

Sequence information

16S sequences

• @ref: 65304
• description: 16S rRNA gene sequence
• accession: KT900247
• database: nuccore

GC content

• @ref: 65304
• GC-content: 48.7
• method: thermal denaturation, midpoint method (Tm)

External links

@ref: 65304

culture collection no.: KACC 18560, CGMCC 1.15399

literature

• topic: Phylogeny
• Pubmed-ID: 29160196
• title: Rheinheimera marina sp. nov., isolated from a deep-sea seamount.
• authors: Wang Q, Sun YW, Liu J, Zhang DC
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijsem.0.002496
• year: 2017
• mesh: Bacterial Typing Techniques, Base Composition, Chromatiaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Pacific Ocean, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA
• topic2: Transcriptome

Reference