Strain identifier

BacDive ID: 158585

Type strain: Yes

Species: Bacillus marinisedimentorum

Strain Designation: NC2-31

Strain history: <- Zongjun Du, Shandong Univ., China

NCBI tax ID(s): 1821260 (species)

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General

@ref: 65297

BacDive-ID: 158585

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-positive, motile

description: Bacillus marinisedimentorum NC2-31 is a facultative anaerobe, Gram-positive, motile bacterium that was isolated from sediment sample.

NCBI tax id

  • NCBI tax id: 1821260
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Zongjun Du, Shandong Univ., China

doi: 10.13145/bacdive158585.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus marinisedimentorum
  • full scientific name: Bacillus marinisedimentorum Guo et al. 2018

@ref: 65297

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus marinisedimentorum

strain designation: NC2-31

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthmotilityflagellum arrangementcell shape
65297positive4.2-10 µm0.7-1.3 µmyesperitrichous
67771yesrod-shaped
67771positive

Culture and growth conditions

culture medium

@refnamegrowthcomposition
65297Marine agar (MA)yes
65297Marine agar (MA)yes0.1 5 (w/v) KNO3

culture temp

@refgrowthtypetemperaturerange
65297positivegrowth20-45
65297positiveoptimum37mesophilic
67771positivegrowth33mesophilic

culture pH

@refabilitytypepHPH range
65297positivegrowth6.5-8.5alkaliphile
65297positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65297facultative anaerobe
67771aerobe

spore formation

@refspore formation
65297no
67771no

halophily

@refsaltgrowthtested relationconcentration
65297NaClpositivegrowth1-7 %(w/v)
65297NaClpositiveoptimum2-3 %(w/v)

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6529715588D-malate+oxidation
6529730089acetate+oxidation
6529713705acetoacetate+oxidation
6529717925alpha-D-glucose+oxidation
6529729016arginine-hydrolysis
6529785146carboxymethylcellulose-hydrolysis
6529717057cellobiose+builds acid from
6529717057cellobiose+oxidation
6529716947citrate-assimilation
6529715824D-fructose+builds acid from
6529717634D-glucose+builds acid from
6529762318D-lyxose+builds acid from
6529716899D-mannitol+builds acid from
6529716024D-mannose+builds acid from
6529716988D-ribose+builds acid from
6529717924D-sorbitol+builds acid from
6529716443D-tagatose+builds acid from
6529765327D-xylose+builds acid from
6529723652dextrin+oxidation
652974853esculin+builds acid from
652975291gelatin-hydrolysis
6529728066gentiobiose+oxidation
6529732323glucuronamide+oxidation
6529730849L-arabinose+builds acid from
6529725094lysine-hydrolysis
6529717306maltose+builds acid from
6529717306maltose+oxidation
65297320061methyl alpha-D-glucopyranoside+builds acid from
65297506227N-acetylglucosamine+builds acid from
6529717632nitrate+reduction
6529718257ornithine-hydrolysis
65297potassium 2-dehydro-D-gluconate+builds acid from
65297potassium 5-dehydro-D-gluconate+builds acid from
6529717272propionate+oxidation
6529717164stachyose+oxidation
6529728017starch-hydrolysis
6529717992sucrose+builds acid from
6529717992sucrose+oxidation
6529727082trehalose+builds acid from
6529727082trehalose+oxidation
6529727897tryptophan-hydrolysis
6529732528turanose+builds acid from
6529732528turanose+oxidation
6529753424tween 20-hydrolysis
6529753423tween 40-hydrolysis
6529753425tween 60-hydrolysis
6529753426tween 80-hydrolysis
6529716199urea-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
652976472lincomycinyesyes2 µg (disc)
652977731ofloxacinyesyes5 µg (disc)
6529728077rifampicinyesyes5 µg (disc)
6529717334penicillinyesyes10 µg (disc)
6529728971ampicillinyesyes10 µg (disc)
6529728864tobramycinyesyes10 µg (disc)
6529717698chloramphenicolyesyes30 µg (disc)
652977507neomycinyesyes30 µg (disc)
6529728001vancomycinyesyes30 µg (disc)
65297204928cefotaximeyesyes30 µg (disc)
6529717833gentamicinyesyes10 µg (disc)
6529717076streptomycinyesyes10 µg (disc)
652976104kanamycinyesyes30 µg (disc)
6529727902tetracyclineyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6529715688acetoinno
6529716136hydrogen sulfideno
6529735581indoleno

metabolite tests

  • @ref: 65297
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
65297catalase-1.11.1.6
65297cytochrome oxidase-1.9.3.1
65297N-acetyl-beta-glucosaminidase-3.2.1.52
65297lipase (C 14)-
65297leucine arylamidase-3.4.11.1
65297valine arylamidase-
65297cystine arylamidase-3.4.11.3
65297alpha-chymotrypsin-3.4.21.1
65297trypsin-3.4.21.4
65297acid phosphatase-3.1.3.2
65297naphthol-AS-BI-phosphohydrolase-
65297alpha-galactosidase-3.2.1.22
65297beta-galactosidase-3.2.1.23
65297beta-glucuronidase-3.2.1.31
65297beta-glucosidase-3.2.1.21
65297alpha-glucosidase-3.2.1.20
65297alpha-mannosidase-3.2.1.24
65297beta-D-fucosidase-3.2.1.38
65297alkaline phosphatase+3.1.3.1
65297esterase (C 4)+
65297esterase Lipase (C 8)+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
65297sediment samplecoast of WeihaiChinaCHNAsia37.4188121.997marine agar 22167 days28dilution plating method
67771From marine sedimentcoast of WeihaiChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Sediment
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_5217.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_2535;97_3096;98_3888;99_5217&stattab=map
  • Last taxonomy: Bacillus marinisedimentorum subclade
  • 16S sequence: KX548355
  • Sequence Identity:
  • Total samples: 880
  • soil counts: 423
  • aquatic counts: 226
  • animal counts: 190
  • plant counts: 41

Sequence information

16S sequences

  • @ref: 65297
  • description: 16S rRNA gene sequence
  • accession: KX548355
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus marinisedimentorum NC2-312744054838draftimg1821260
67771Bacillus marinisedimentorum NC2-31GCA_001644195contigncbi1821260

GC content

@refGC-contentmethod
6529746.3genome sequence analysis
6777146.3

External links

@ref: 65297

culture collection no.: KCTC 33721, MCCC 1K01239

literature

  • topic: Phylogeny
  • Pubmed-ID: 29134941
  • title: Bacillus marinisedimentorum sp. nov., isolated from marine sediment.
  • authors: Guo LY, Ling SK, Li CM, Chen GJ, Du ZJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002482
  • year: 2017
  • mesh: Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65297Ling-Yun Guo, Si-Kai Ling, Chang-Ming Li, Guan-Jun Chen, Zong-Jun DuBacillus marinisedimentorum sp. nov., isolated from marine sediment10.1099/ijsem.0.002482IJSEM 68: 198-203 201829134941
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/