Strain identifier
BacDive ID: 158578
Type strain:
Species: Melaminivora jejuensis
Strain Designation: KBB12
Strain history: J.-Y. Kim KBB12.
NCBI tax ID(s): 1267217 (species)
General
@ref: 65285
BacDive-ID: 158578
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming
description: Melaminivora jejuensis KBB12 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from swinery sludge.
NCBI tax id
- NCBI tax id: 1267217
- Matching level: species
strain history
- @ref: 67770
- history: J.-Y. Kim KBB12.
doi: 10.13145/bacdive158578.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Comamonadaceae
- genus: Melaminivora
- species: Melaminivora jejuensis
- full scientific name: Melaminivora jejuensis Kim et al. 2018
@ref: 65285
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Comamonadaceae
genus: Melaminivora
species: Melaminivora jejuensis
strain designation: KBB12
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | flagellum arrangement | confidence |
---|---|---|---|---|---|---|---|
65285 | negative | 2-3.5 µm | 0.5-0.7 µm | rod-shaped | yes | polar | |
69480 | yes | 95.419 | |||||
69480 | negative | 100 |
colony morphology
- @ref: 65285
- colony size: 1.5 mm
- colony color: pale yellow
- colony shape: circular
- incubation period: 3 days
- medium used: tryptic soy agar
Culture and growth conditions
culture medium
- @ref: 65285
- name: tryptic soy agar
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65285 | positive | optimum | 30-37 | mesophilic |
65285 | positive | growth | 15-45 | |
65285 | no | growth | <10 | psychrophilic |
65285 | no | growth | >50 | thermophilic |
67770 | positive | growth | 37 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
65285 | positive | optimum | 7-8 | |
65285 | positive | growth | 6-9 | alkaliphile |
65285 | no | growth | <5 | |
65285 | no | growth | >10 |
Physiology and metabolism
oxygen tolerance
- @ref: 65285
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69480 | no | 99.99 |
69481 | no | 100 |
halophily
- @ref: 65285
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 0-1 %(w/v)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65285 | 17128 | adipate | + | assimilation |
65285 | 8295 | beta-hydroxybutyrate | + | assimilation |
65285 | 73706 | bromosuccinate | + | assimilation |
65285 | casein | - | hydrolysis | |
65285 | 18333 | D-arabitol | + | assimilation |
65285 | 16024 | D-mannose | + | assimilation |
65285 | 17924 | D-sorbitol | + | assimilation |
65285 | 4853 | esculin | - | hydrolysis |
65285 | 5291 | gelatin | + | hydrolysis |
65285 | 24265 | gluconate | + | assimilation |
65285 | 32323 | glucuronamide | + | assimilation |
65285 | 6359 | lactulose | + | assimilation |
65285 | 25115 | malate | + | assimilation |
65285 | 17306 | maltose | + | assimilation |
65285 | 17268 | myo-inositol | + | assimilation |
65285 | 63153 | N-acetyl-D-mannosamine | + | assimilation |
65285 | 28037 | N-acetylgalactosamine | + | assimilation |
65285 | 506227 | N-acetylglucosamine | + | assimilation |
65285 | 18394 | palatinose | + | assimilation |
65285 | 16634 | raffinose | + | assimilation |
65285 | 28017 | starch | - | hydrolysis |
65285 | 143136 | succinamate | + | assimilation |
65285 | 17992 | sucrose | + | assimilation |
65285 | 53424 | tween 20 | + | hydrolysis |
65285 | 53423 | tween 40 | + | hydrolysis |
65285 | 53425 | tween 60 | - | hydrolysis |
65285 | 53426 | tween 80 | + | hydrolysis |
65285 | 18186 | tyrosine | - | hydrolysis |
65285 | 16199 | urea | - | hydrolysis |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. | is resistant | resistance conc. |
---|---|---|---|---|---|---|---|
65285 | 28971 | ampicillin | yes | yes | 10 µg (disc) | ||
65285 | 17833 | gentamicin | yes | yes | 10 µg (disc) | ||
65285 | 18208 | penicillin g | yes | yes | 10 Unit (disc) | ||
65285 | 3542 | cephalothin | yes | yes | 30 µg (disc) | ||
65285 | 17698 | chloramphenicol | yes | yes | 30 µg (disc) | ||
65285 | 6104 | kanamycin | yes | yes | 30 µg (disc) | ||
65285 | 7507 | neomycin | yes | yes | 30 µg (disc) | ||
65285 | 27902 | tetracycline | yes | yes | 30 µg (disc) | ||
65285 | 8309 | polymyxin b | yes | yes | 300 Unit (disc) | ||
65285 | 48923 | erythromycin | yes | yes | 15 µg (disc) | ||
65285 | 6472 | lincomycin | yes | yes | 2 µg (disc) | ||
65285 | 28077 | rifampicin | yes | yes | 5 µg (disc) | ||
65285 | 17076 | streptomycin | yes | yes | 10 µg (disc) | ||
65285 | 16869 | oleandomycin | yes | yes | 15 µg (disc) | ||
65285 | 100147 | nalidixic acid | yes | yes | 30 µg (disc) | ||
65285 | 28368 | novobiocin | yes | yes | 30 µg (disc) |
enzymes
@ref | value | activity | ec |
---|---|---|---|
65285 | alkaline phosphatase | + | 3.1.3.1 |
65285 | esterase (C 4) | + | |
65285 | esterase Lipase (C 8) | + | |
65285 | leucine arylamidase | + | 3.4.11.1 |
65285 | valine arylamidase | + | |
65285 | cystine arylamidase | + | 3.4.11.3 |
65285 | naphthol-AS-BI-phosphohydrolase | + | |
65285 | lipase (C 14) | - | |
65285 | trypsin | - | 3.4.21.4 |
65285 | alpha-chymotrypsin | - | 3.4.21.1 |
65285 | alpha-galactosidase | - | 3.2.1.22 |
65285 | beta-galactosidase | - | 3.2.1.23 |
65285 | beta-glucuronidase | - | 3.2.1.31 |
65285 | alpha-glucosidase | - | 3.2.1.20 |
65285 | beta-glucosidase | - | 3.2.1.21 |
65285 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
65285 | alpha-mannosidase | - | 3.2.1.24 |
65285 | alpha-fucosidase | - | 3.2.1.51 |
65285 | arginine dihydrolase | - | 3.5.3.6 |
65285 | lysine decarboxylase | - | 4.1.1.18 |
65285 | ornithine decarboxylase | - | 4.1.1.17 |
fatty acid profile
fatty acids
@ref fatty acid percentage 65285 C10:0 1.2 65285 C10:0 3OH 5.1 65285 C12:0 3.6 65285 C14:0 1.3 65285 C16:0 25.2 65285 C17:0 cyclo 1.3 65285 C18:1ω7c 16.1 65285 C18:1ω7c 11-methyl 0.8 65285 C13:0 3-OH/iso-C15:1 I/H 0.5 65285 C15:0 iso 2OH and/or C16:1ω7c 42.9 65285 C19:1 cyclo ω10c and/or C19:1ω6c 0.8 - type of FA analysis: whole cell analysis
- incubation medium: tryptic soy agar
- agar/liquid: agar
- incubation temperature: 30
- incubation time: 2
- software version: Sherlock 4.5
- library/peak naming table: TSBA5
- system: MIS MIDI
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture composition | isolation procedure |
---|---|---|---|---|---|---|---|---|
65285 | swinery sludge | Jeju | Republic of Korea | KOR | Asia | BH medium | l-1 in distilled water: MgSO4 x 7H2O, 0.409 g; CaCl2 x 2H2O, 0.0265 g; KH2PO4, 1 g; NH4NO3, 1 g; Na2HPO4 x 12H2O, 6 g; FeCl3 x 6H2O, 0.0833 g; NaHCO3, 0.2 g, and Na2S2O3, 4.0 g, plus 1 ml trace element solution | Enrichment and isolation were performed using a slightly modified version of the medium used for sulfur-oxidizing bacteria, designated BH medium. The strain was subcultivated on tryptic soy agar at 30°C and preserved in a glycerol solution (20 %, v/v) at -80°C. |
67770 | Swinery waste in Jeju | Republic of Korea | KOR | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Agriculture | #Livestock (Husbandry) |
#Environmental | #Terrestrial | #Mud (Sludge) |
#Host | #Mammals | #Suidae (Pig,Swine) |
#Engineered | #Waste |
Sequence information
16S sequences
- @ref: 65285
- description: 16S rRNA gene sequence
- accession: JX997988
- database: nuccore
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Melaminivora jejuensis KCTC 32230 | GCA_017811175 | contig | ncbi | 1267217 |
66792 | Melaminivora jejuensis strain KCTC 32230 | 1267217.3 | wgs | patric | 1267217 |
GC content
- @ref: 67770
- GC-content: 69.6
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | yes | 90.305 | yes |
gram-positive | no | 98.587 | yes |
anaerobic | no | 98.69 | yes |
aerobic | yes | 94.668 | yes |
halophile | no | 91.51 | yes |
spore-forming | no | 95.06 | no |
glucose-util | no | 77.453 | no |
flagellated | yes | 72.82 | yes |
thermophile | no | 90.069 | no |
glucose-ferment | no | 89.175 | no |
External links
@ref: 65285
culture collection no.: KCTC 32230, JCM 18740
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 29111968 | Melaminivora jejuensis sp. nov., isolated from Swinery waste. | Kim JY, Park SH, Lee DH, Song G, Kim YJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002294 | 2017 | Animals, Bacterial Typing Techniques, Base Composition, Comamonadaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Manure/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Swine, Ubiquinone/chemistry | Transcriptome |
Phylogeny | 31046896 | Pulveribacter suum gen. nov., sp. nov., isolated from a pig farm dust collector. | Heo J, Cho HY, Heo I, Hong SB, Kim JS, Kwon SW, Kim SJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003082 | 2019 | Animals, Bacterial Typing Techniques, Base Composition, Comamonadaceae/*classification/isolation & purification, DNA, Bacterial/genetics, *Dust, *Farms, Fatty Acids/chemistry, Genes, Bacterial, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Swine, Ubiquinone/chemistry | Transcriptome |
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
65285 | Ji-Young Kim, So-Hyun Park, Dong-Heon Lee, Gwanpil Song, Young-Ju Kim | Melaminivora jejuensis sp. nov., isolated from Swinery waste | 10.1099/ijsem.0.002294 | IJSEM 68: 9-13 2018 | 29111968 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 |