Strain identifier

BacDive ID: 158577

Type strain: Yes

Species: Stutzerimonas tarimensis

Strain Designation: MA-69

Strain history: <- Erkin Rahman, Xinjiang Univ.

NCBI tax ID(s): 1507735 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 8.1 (current version)

General

@ref: 65283

BacDive-ID: 158577

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Stutzerimonas tarimensis MA-69 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from From Ugan rivers of Tarim basin.

NCBI tax id

  • NCBI tax id: 1507735
  • Matching level: species

strain history

@refhistory
67770E. Rahman MA-69.
67771<- Erkin Rahman, Xinjiang Univ.

doi: 10.13145/bacdive158577.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Stutzerimonas
  • species: Stutzerimonas tarimensis
  • full scientific name: Stutzerimonas tarimensis (Anwar et al. 2017) Gomila et al. 2022

  • synonyms

    • @ref: 20215
    • synonym: Pseudomonas tarimensis

@ref: 65283

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas tarimensis

strain designation: MA-69

type strain: yes

Morphology

cell morphology

  • @ref: 65283
  • gram stain: negative
  • cell length: 1.5-2 µm
  • cell width: 0.5-0.7 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: monopolar

colony morphology

  • @ref: 65283
  • colony size: 1 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: MA agar

Culture and growth conditions

culture medium

  • @ref: 65283
  • name: MA agar
  • growth: yes
  • composition: 5 g peptone, 1 g yeast extract, 0.1 g ferric citrate, 19.45 g NaCl, 8.8 g MgCl2, 3.24 g Na2SO4, 1.8 g CaCl2 , 0.55 g KCl, 15 g agar and 10 ml trace element solution per litre distilled water, pH 7.4-7.8

culture temp

@refgrowthtypetemperaturerange
65283positivegrowth25-37mesophilic
65283positiveoptimum30mesophilic
67770positivegrowth30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
65283positivegrowth6-9alkaliphile
65283positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65283aerobe
67771aerobe

spore formation

  • @ref: 65283
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
65283NaClpositivegrowth0-3 %(w/v)
65283NaClpositiveoptimum1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6528316651(S)-lactate+carbon source
652831 % sodium lactate-carbon source
65283167632-oxobutanoate-carbon source
65283309162-oxoglutarate+carbon source
65283370543-hydroxybutyrate-carbon source
65283739183-O-methyl-D-glucose-carbon source
65283181014-hydroxyphenylacetic acid-carbon source
6528330089acetate-carbon source
6528313705acetoacetate-carbon source
6528317925alpha-D-glucose-carbon source
6528336219alpha-lactose-carbon source
65283645522-hydroxybutyrate-carbon source
65283161680aztreonam-carbon source
6528373706bromosuccinate-carbon source
6528316947citrate-carbon source
6528318333D-arabitol-carbon source
6528329990D-aspartate-carbon source
6528317057cellobiose-carbon source
6528315824D-fructose+carbon source
6528378697D-fructose 6-phosphate+carbon source
6528328847D-fucose-carbon source
6528312936D-galactose-carbon source
6528318024D-galacturonic acid-carbon source
6528317634D-glucose-carbon source
6528317634D-glucose-energy source
6528314314D-glucose 6-phosphate-carbon source
6528315748D-glucuronate-carbon source
6528315748D-glucuronate+carbon source
6528315588D-malate-carbon source
6528316899D-mannitol-carbon source
6528316899D-mannitol-energy source
6528316024D-mannose-carbon source
6528316024D-mannose-energy source
6528328053melibiose-carbon source
6528316634raffinose-carbon source
6528333801D-saccharate-carbon source
6528316523D-serine-carbon source
6528317924D-sorbitol-carbon source
6528316551D-trehalose-carbon source
6528332528turanose-carbon source
6528323652dextrin-carbon source
6528315740formate-carbon source
6528371321fusidate-carbon source
6528316537galactarate-carbon source
6528316865gamma-aminobutyric acid-carbon source
652835291gelatin-carbon source
652835291gelatin-hydrolysis
6528328066gentiobiose-carbon source
6528317234glucose-fermentation
6528332323glucuronamide+carbon source
6528317754glycerol-carbon source
6528370744glycine-proline-carbon source
6528317596inosine-carbon source
6528316977L-alanine+carbon source
6528330849L-arabinose-carbon source
6528330849L-arabinose-energy source
6528316467L-arginine-carbon source
6528329991L-aspartate-carbon source
6528318287L-fucose-carbon source
6528317464L-galactonic acid gamma-lactone-carbon source
6528329985L-glutamate-carbon source
6528315971L-histidine-carbon source
6528315589L-malate+carbon source
6528318183L-pyroglutamic acid-carbon source
6528362345L-rhamnose-carbon source
6528317115L-serine-carbon source
6528348607lithium chloride-carbon source
6528317306maltose-carbon source
6528317306maltose-energy source
6528374611methyl (R)-lactate+carbon source
65283320055methyl beta-D-glucopyranoside-carbon source
6528351850methyl pyruvate-carbon source
6528317268myo-inositol-carbon source
6528363153N-acetyl-D-mannosamine-carbon source
6528328037N-acetylgalactosamine-carbon source
65283506227N-acetylglucosamine-carbon source
6528335418n-acetylneuraminate-carbon source
65283100147nalidixic acid-carbon source
6528317632nitrate-reduction
6528317309pectin-carbon source
6528375248potassium tellurite-carbon source
6528317272propionate-carbon source
6528326490quinate+carbon source
6528317814salicin-carbon source
6528375229sodium bromate-carbon source
6528364103sodium butyrate-carbon source
6528317164stachyose-carbon source
6528328017starch+hydrolysis
6528317992sucrose-carbon source
6528327897tryptophan-energy source
6528353423tween 40-carbon source
6528316199urea-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.
6528317833gentamicinyesyes10 µg/mL
6528317076streptomycinyesyes10 µg/mL
6528327902tetracyclineyesyes30 µg/mL
652836104kanamycinyesyes30 µg/mL
6528317698chloramphenicolyesyes50 µg/mL

metabolite production

@refChebi-IDmetaboliteproduction
6528315688acetoinno
6528335581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testindole test
6528317234glucose-
6528315688acetoin-
6528335581indole-

enzymes

@refvalueactivityec
65283tryptophan deaminase-4.1.99.1
65283catalase+1.11.1.6
65283cytochrome oxidase+1.9.3.1
65283alkaline phosphatase+3.1.3.1
65283esterase+
65283esterase Lipase (C 8)+
65283leucine arylamidase+3.4.11.1
65283valine arylamidase+
65283trypsin+3.4.21.4
65283naphthol-AS-BI-phosphohydrolase+
65283arginine dihydrolase-3.5.3.6
65283ornithine decarboxylase-4.1.1.17
65283lysine decarboxylase-4.1.1.18
65283phenylalanine deaminase-4.3.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65283C10:0 3-OH2.6
    65283C12:06.3
    65283C12:0 3OH3.5
    65283C14:01.1
    65283C16:014.6
    65283C19:0 cyclo ω8c19.9
    65283C16:1ω6c and/or C16:1ω7c25.7
    65283C18?17c and/or C18:1ω6c24
  • type of FA analysis: whole cell analysis
  • incubation medium: tryptic soy broth
  • incubation temperature: 30
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedurehost species
67771From Ugan rivers of Tarim basinXinjiangChinaCHNAsia
65283sample from the storage liquid in the stem of Populus euphraticaancient Ugan River in Xin-jiang Uyghur Autonomous RegionChinaCHNAsia41.107585.0644MA medium5 g peptone, 1 g yeast extract, 0.1 g ferric citrate, 19.45 g NaCl, 8.8 g MgCl2, 3.24 g Na2SO4, 1.8 g CaCl2 , 0.55 g KCl, 15 g agar and 10 ml trace element solution per litre distilled water, pH 7.4-7.8. The trace element solution contained (per litre distilled water): 16 g NaHCO3, 0.16 g NH4NO3, 0.8 g Na2HPO4, 3.4 g SrCl2, 2.2 g boric acid, 0.4 g sodium silicate, 8 g KBr and 0.24 g NaF.10 days30serial dilutions of the samples with sterilized 0.85 % saline solution were plated onto MA medium. The plates were incubated for 10 days at 30°C.
67770Storage liquid in the stems of Populuseuphratica trees at the ancient Ugan River in XinjiangChinaCHNAsiaPopuluseuphratica

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Plant#Stem (Branch)
#Host#Plants#Tree

taxonmaps

  • @ref: 69479
  • File name: preview.99_109371.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_18646;97_35816;98_74254;99_109371&stattab=map
  • Last taxonomy: Pseudomonas tarimensis subclade
  • 16S sequence: KC709664
  • Sequence Identity:
  • Total samples: 32
  • soil counts: 6
  • aquatic counts: 10
  • animal counts: 14
  • plant counts: 2

Sequence information

16S sequences

  • @ref: 65283
  • description: 16S rRNA gene sequence
  • accession: KC709664
  • database: nuccore

GC content

  • @ref: 65283
  • GC-content: 64.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65283

culture collection no.: CCTCC AB 2013065, KCTC 42447, JCM 19137

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28984556Pseudomonas tarimensis sp. nov., an endophytic bacteria isolated from Populus euphratica.Anwar N, Rozahon M, Zayadan B, Mamtimin H, Abdurahman M, Kurban M, Abdurusul M, Mamtimin T, Abdukerim M, Rahman EInt J Syst Evol Microbiol10.1099/ijsem.0.0022952017Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Stems/*microbiology, Populus/*microbiology, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome
Phylogeny30950783Pseudomonas urumqiensis sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia.Zou Y, He S, Sun Y, Zhang X, Liu Y, Cheng QInt J Syst Evol Microbiol10.1099/ijsem.0.0033902019Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fabaceae/*microbiology, Fatty Acids/chemistry, Genes, Bacterial, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil MicrobiologyTranscriptome
Phylogeny31429815Pseudomonas mangiferae sp. nov., isolated from bark of mango tree in Thailand.Anurat P, Duangmal K, Srisuk NInt J Syst Evol Microbiol10.1099/ijsem.0.0036572019Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Mangifera/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Bark/*microbiology, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Ubiquinone/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65283Nusratgul Anwar, Manziram Rozahon, Bolatkhan Zayadan, Hormathan Mamtimin, Mehfuzem Abdurahman, Marygul Kurban, Mihribangul Abdurusul, Tursunay Mamtimin, Muhtar Abdukerim, Erkin RahmanPseudomonas tarimensis sp. nov., an endophytic bacteria isolated from Populus euphratica10.1099/ijsem.0.002295IJSEM 67: 4372-4378 201728984556
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/