Strain identifier

BacDive ID: 158560

Type strain: Yes

Species: Aeribacillus composti

Strain Designation: N.8

Strain history: <- Annarita Poli, Consiglio Nazionale delle Ricerche(CNR)

NCBI tax ID(s): 1868734 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 8.1 (current version)

General

@ref: 65257

BacDive-ID: 158560

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, haloalkaliphilic, spore-forming, thermophilic, Gram-positive, rod-shaped, colony-forming

description: Aeribacillus composti N.8 is an obligate aerobe, haloalkaliphilic, spore-forming bacterium that forms circular colonies and was isolated from compost sample.

NCBI tax id

  • NCBI tax id: 1868734
  • Matching level: species

strain history

@refhistory
67770A. Poli; Natl. Res. Counc. (C.N.R.), Inst. of Biomol. Chem. (I.C.B.), Italy; N.8.
67771<- Annarita Poli, Consiglio Nazionale delle Ricerche(CNR)

doi: 10.13145/bacdive158560.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Aeribacillus
  • species: Aeribacillus composti
  • full scientific name: Aeribacillus composti Finore et al. 2017

@ref: 65257

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Aeribacillus

species: Aeribacillus composti

strain designation: N.8

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
65257positive1.5-5 µm0.5-0.6 µmrod-shapedno
69480yes92.369
69480positive100

colony morphology

  • @ref: 65257
  • colony size: 1 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 1 day
  • medium used: solid medium

Culture and growth conditions

culture medium

  • @ref: 65257
  • name: solid medium
  • growth: yes
  • composition: growth medium A containing (l-1): peptone, 8 g; yeast extract 4 g and NaCl 2 g.

culture temp

@refgrowthtypetemperaturerange
65257positivegrowth50-65thermophilic
65257positiveoptimum60thermophilic
67770positivegrowth55thermophilic
67771positivegrowth60thermophilic

culture pH

@refabilitytypepHPH range
65257positivegrowth5.5-10alkaliphile
65257positiveoptimum9

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65257obligate aerobe
67771aerobe

spore formation

@reftype of sporespore formationconfidence
65257endosporeyes
69480yes99.999
69481yes100

halophily

@refhalophily levelsaltgrowthtested relationconcentration
65257haloalkaliphilicNaClpositivegrowth2-9 %(w/v)
65257haloalkaliphilicNaClpositiveoptimum6 %(w/v)

observation

@refobservation
65257Positive for aminopeptidase test.
67770quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6525716651(S)-lactate+carbon source
65257288162-deoxy-d-ribose+carbon source
65257182404-hydroxy-L-proline+nitrogen source
6525785146carboxymethylcellulose-hydrolysis
65257casein-hydrolysis
6525717108D-arabinose+assimilation
6525717634D-glucose-builds gas from
6525717634D-glucose+builds acid from
6525717634D-glucose+carbon source
6525716899D-mannitol+carbon source
6525716024D-mannose+carbon source
6525716988D-ribose+carbon source
6525717924D-sorbitol+carbon source
6525765327D-xylose+carbon source
6525729806fumarate+carbon source
652575291gelatin-hydrolysis
6525717754glycerol+carbon source
6525715428glycine+nitrogen source
65257606565hippurate+hydrolysis
6525716977L-alanine+nitrogen source
6525730849L-arabinose+assimilation
6525716467L-arginine+nitrogen source
6525717196L-asparagine+nitrogen source
6525729991L-aspartate+nitrogen source
6525718287L-fucose+carbon source
6525729985L-glutamate+nitrogen source
6525718050L-glutamine+nitrogen source
6525715971L-histidine+nitrogen source
6525717191L-isoleucine+nitrogen source
6525715603L-leucine+nitrogen source
6525718019L-lysine+nitrogen source
6525716643L-methionine+nitrogen source
6525715729L-ornithine+nitrogen source
6525717295L-phenylalanine+nitrogen source
6525762345L-rhamnose+carbon source
6525717115L-serine+nitrogen source
6525716857L-threonine+nitrogen source
6525716828L-tryptophan+nitrogen source
6525717895L-tyrosine+hydrolysis
6525717895L-tyrosine+nitrogen source
6525716414L-valine+nitrogen source
6525717306maltose+carbon source
6525761993maltotriose+carbon source
6525728044phenylalanine-hydrolysis
6525715361pyruvate+carbon source
6525728017starch-hydrolysis
6525730031succinate+carbon source
6525717992sucrose+carbon source
6525727082trehalose+carbon source
6525716199urea-hydrolysis
6525737166xylan-hydrolysis
6525717151xylitol+carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6525728669bacitracinyesyes10 Unit
6525717334penicillinyesyes10 Unit
6525771321fusidateyesyes10 µg (disc)
6525717698chloramphenicolyesyes10 µg (disc)
652576472lincomycinyesyes15 µg (disc)
6525717076streptomycinyesyes25 µg (disc)
6525728971ampicillinyesyes25 µg (disc)
6525717833gentamicinyesyes30 µg (disc)
6525728368novobiocinyesyes30 µg (disc)
6525748923erythromycinyesyes30 µg (disc)
6525727902tetracyclineyesyes30 µg (disc)
652577507neomycinyesyes30 µg (disc)
6525728001vancomycinyesyes30 µg (disc)
652576104kanamycinyesyes30 µg (disc)
652577660nystatinyesyes100 µg (disc)

metabolite production

  • @ref: 65257
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 65257
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
65257catalase+1.11.1.6
65257cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture temperatureisolation procedure
65257compost sampleComposting Experimental Centre (CESCO) in Cilento National Park, SalernoItalyITAEurope40.288515.3608growth medium A(l-1): peptone, 8 g; yeast extract 4 g and NaCl, 2 g60The compost sample (1 g) was dissolved in 200 ml sterilised 50 mM phosphate buffer (pH 7.0) with agitation (120 r.p.m.) for 24 h at 60°C. The suspension obtained was used to inoculate 10 ml growth medium A. After 48 h at 60°C, the growth that had occurred was used as an inoculum for the same solid medium obtained by adding agar [1.8 % (w/v)]. After 48 h incubation at 60°C growth had occurred and colonies were purified by using the serial dilution-plating method at 60°C followed by restreaking on
67770Curing step of an olive mill pomace compost sample collected at the Composting Experimental CentreSalernoItalyITAEurope
67771From compostLaurino, Salerno-Experimental Composting CenterItalyITAEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Biodegradation
  • Cat3: #Composting

taxonmaps

  • @ref: 69479
  • File name: preview.99_6456.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_3055;97_3753;98_4764;99_6456&stattab=map
  • Last taxonomy: Aeribacillus
  • 16S sequence: LT594972
  • Sequence Identity:
  • Total samples: 9786
  • soil counts: 1959
  • aquatic counts: 1682
  • animal counts: 5491
  • plant counts: 654

Sequence information

16S sequences

  • @ref: 65257
  • description: 16S rRNA gene sequence
  • accession: LT594972
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aeribacillus composti KCTC 33824GCA_007827555scaffoldncbi1868734
66792Aeribacillus composti strain KCTC 338241868734.3wgspatric1868734
66792Aeribacillus composti KCTC 338242802428823draftimg1868734

GC content

  • @ref: 65257
  • GC-content: 40.5
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes83.148yes
flagellatedyes89.617no
gram-positiveyes80.895no
anaerobicno96.54no
aerobicyes88.956yes
halophileno72.203no
spore-formingyes96.24yes
glucose-utilyes85.628no
thermophileyes94.209yes
glucose-fermentno93.662no

External links

@ref: 65257

culture collection no.: KCTC 33824, JCM 31580

literature

  • topic: Phylogeny
  • Pubmed-ID: 28984237
  • title: Aeribacillus composti sp. nov., a thermophilic bacillus isolated from olive mill pomace compost.
  • authors: Finore I, Gioiello A, Leone L, Orlando P, Romano I, Nicolaus B, Poli A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002391
  • year: 2017
  • mesh: Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, *Composting, DNA, Bacterial/genetics, Fatty Acids/chemistry, Italy, Nucleic Acid Hybridization, Olea/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65257Ilaria Finore, Alessia Gioiello, Luigi Leone, Pierangelo Orlando, Ida Romano, Barbara Nicolaus, Annarita PoliAeribacillus composti sp. nov., a thermophilic bacillus isolated from olive mill pomace compost10.1099/ijsem.0.002391IJSEM 67: 4830-4835 201728984237
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1