Strain identifier
BacDive ID: 158560
Type strain:
Species: Aeribacillus composti
Strain Designation: N.8
Strain history: <- Annarita Poli, Consiglio Nazionale delle Ricerche(CNR)
NCBI tax ID(s): 1868734 (species)
General
@ref: 65257
BacDive-ID: 158560
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, haloalkaliphilic, spore-forming, thermophilic, Gram-positive, rod-shaped, colony-forming
description: Aeribacillus composti N.8 is an obligate aerobe, haloalkaliphilic, spore-forming bacterium that forms circular colonies and was isolated from compost sample.
NCBI tax id
- NCBI tax id: 1868734
- Matching level: species
strain history
@ref | history |
---|---|
67770 | A. Poli; Natl. Res. Counc. (C.N.R.), Inst. of Biomol. Chem. (I.C.B.), Italy; N.8. |
67771 | <- Annarita Poli, Consiglio Nazionale delle Ricerche(CNR) |
doi: 10.13145/bacdive158560.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Aeribacillus
- species: Aeribacillus composti
- full scientific name: Aeribacillus composti Finore et al. 2017
@ref: 65257
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Caryophanales
family: Bacillaceae
genus: Aeribacillus
species: Aeribacillus composti
strain designation: N.8
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
65257 | positive | 1.5-5 µm | 0.5-0.6 µm | rod-shaped | no | |
69480 | yes | 92.369 | ||||
69480 | positive | 100 |
colony morphology
- @ref: 65257
- colony size: 1 mm
- colony color: cream
- colony shape: circular
- incubation period: 1 day
- medium used: solid medium
Culture and growth conditions
culture medium
- @ref: 65257
- name: solid medium
- growth: yes
- composition: growth medium A containing (l-1): peptone, 8 g; yeast extract 4 g and NaCl 2 g.
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65257 | positive | growth | 50-65 | thermophilic |
65257 | positive | optimum | 60 | thermophilic |
67770 | positive | growth | 55 | thermophilic |
67771 | positive | growth | 60 | thermophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
65257 | positive | growth | 5.5-10 | alkaliphile |
65257 | positive | optimum | 9 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
65257 | obligate aerobe |
67771 | aerobe |
spore formation
@ref | type of spore | spore formation | confidence |
---|---|---|---|
65257 | endospore | yes | |
69480 | yes | 99.999 | |
69481 | yes | 100 |
halophily
@ref | halophily level | salt | growth | tested relation | concentration |
---|---|---|---|---|---|
65257 | haloalkaliphilic | NaCl | positive | growth | 2-9 %(w/v) |
65257 | haloalkaliphilic | NaCl | positive | optimum | 6 %(w/v) |
observation
@ref | observation |
---|---|
65257 | Positive for aminopeptidase test. |
67770 | quinones: MK-7 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65257 | 16651 | (S)-lactate | + | carbon source |
65257 | 28816 | 2-deoxy-d-ribose | + | carbon source |
65257 | 18240 | 4-hydroxy-L-proline | + | nitrogen source |
65257 | 85146 | carboxymethylcellulose | - | hydrolysis |
65257 | casein | - | hydrolysis | |
65257 | 17108 | D-arabinose | + | assimilation |
65257 | 17634 | D-glucose | - | builds gas from |
65257 | 17634 | D-glucose | + | builds acid from |
65257 | 17634 | D-glucose | + | carbon source |
65257 | 16899 | D-mannitol | + | carbon source |
65257 | 16024 | D-mannose | + | carbon source |
65257 | 16988 | D-ribose | + | carbon source |
65257 | 17924 | D-sorbitol | + | carbon source |
65257 | 65327 | D-xylose | + | carbon source |
65257 | 29806 | fumarate | + | carbon source |
65257 | 5291 | gelatin | - | hydrolysis |
65257 | 17754 | glycerol | + | carbon source |
65257 | 15428 | glycine | + | nitrogen source |
65257 | 606565 | hippurate | + | hydrolysis |
65257 | 16977 | L-alanine | + | nitrogen source |
65257 | 30849 | L-arabinose | + | assimilation |
65257 | 16467 | L-arginine | + | nitrogen source |
65257 | 17196 | L-asparagine | + | nitrogen source |
65257 | 29991 | L-aspartate | + | nitrogen source |
65257 | 18287 | L-fucose | + | carbon source |
65257 | 29985 | L-glutamate | + | nitrogen source |
65257 | 18050 | L-glutamine | + | nitrogen source |
65257 | 15971 | L-histidine | + | nitrogen source |
65257 | 17191 | L-isoleucine | + | nitrogen source |
65257 | 15603 | L-leucine | + | nitrogen source |
65257 | 18019 | L-lysine | + | nitrogen source |
65257 | 16643 | L-methionine | + | nitrogen source |
65257 | 15729 | L-ornithine | + | nitrogen source |
65257 | 17295 | L-phenylalanine | + | nitrogen source |
65257 | 62345 | L-rhamnose | + | carbon source |
65257 | 17115 | L-serine | + | nitrogen source |
65257 | 16857 | L-threonine | + | nitrogen source |
65257 | 16828 | L-tryptophan | + | nitrogen source |
65257 | 17895 | L-tyrosine | + | hydrolysis |
65257 | 17895 | L-tyrosine | + | nitrogen source |
65257 | 16414 | L-valine | + | nitrogen source |
65257 | 17306 | maltose | + | carbon source |
65257 | 61993 | maltotriose | + | carbon source |
65257 | 28044 | phenylalanine | - | hydrolysis |
65257 | 15361 | pyruvate | + | carbon source |
65257 | 28017 | starch | - | hydrolysis |
65257 | 30031 | succinate | + | carbon source |
65257 | 17992 | sucrose | + | carbon source |
65257 | 27082 | trehalose | + | carbon source |
65257 | 16199 | urea | - | hydrolysis |
65257 | 37166 | xylan | - | hydrolysis |
65257 | 17151 | xylitol | + | carbon source |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. | is resistant | resistance conc. |
---|---|---|---|---|---|---|---|
65257 | 28669 | bacitracin | yes | yes | 10 Unit | ||
65257 | 17334 | penicillin | yes | yes | 10 Unit | ||
65257 | 71321 | fusidate | yes | yes | 10 µg (disc) | ||
65257 | 17698 | chloramphenicol | yes | yes | 10 µg (disc) | ||
65257 | 6472 | lincomycin | yes | yes | 15 µg (disc) | ||
65257 | 17076 | streptomycin | yes | yes | 25 µg (disc) | ||
65257 | 28971 | ampicillin | yes | yes | 25 µg (disc) | ||
65257 | 17833 | gentamicin | yes | yes | 30 µg (disc) | ||
65257 | 28368 | novobiocin | yes | yes | 30 µg (disc) | ||
65257 | 48923 | erythromycin | yes | yes | 30 µg (disc) | ||
65257 | 27902 | tetracycline | yes | yes | 30 µg (disc) | ||
65257 | 7507 | neomycin | yes | yes | 30 µg (disc) | ||
65257 | 28001 | vancomycin | yes | yes | 30 µg (disc) | ||
65257 | 6104 | kanamycin | yes | yes | 30 µg (disc) | ||
65257 | 7660 | nystatin | yes | yes | 100 µg (disc) |
metabolite production
- @ref: 65257
- Chebi-ID: 15688
- metabolite: acetoin
- production: no
metabolite tests
- @ref: 65257
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
65257 | catalase | + | 1.11.1.6 |
65257 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture composition | enrichment culture temperature | isolation procedure |
---|---|---|---|---|---|---|---|---|---|---|---|
65257 | compost sample | Composting Experimental Centre (CESCO) in Cilento National Park, Salerno | Italy | ITA | Europe | 40.2885 | 15.3608 | growth medium A | (l-1): peptone, 8 g; yeast extract 4 g and NaCl, 2 g | 60 | The compost sample (1 g) was dissolved in 200 ml sterilised 50 mM phosphate buffer (pH 7.0) with agitation (120 r.p.m.) for 24 h at 60°C. The suspension obtained was used to inoculate 10 ml growth medium A. After 48 h at 60°C, the growth that had occurred was used as an inoculum for the same solid medium obtained by adding agar [1.8 % (w/v)]. After 48 h incubation at 60°C growth had occurred and colonies were purified by using the serial dilution-plating method at 60°C followed by restreaking on |
67770 | Curing step of an olive mill pomace compost sample collected at the Composting Experimental Centre | Salerno | Italy | ITA | Europe | ||||||
67771 | From compost | Laurino, Salerno-Experimental Composting Center | Italy | ITA | Europe |
isolation source categories
- Cat1: #Engineered
- Cat2: #Biodegradation
- Cat3: #Composting
taxonmaps
- @ref: 69479
- File name: preview.99_6456.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_3055;97_3753;98_4764;99_6456&stattab=map
- Last taxonomy: Aeribacillus
- 16S sequence: LT594972
- Sequence Identity:
- Total samples: 9786
- soil counts: 1959
- aquatic counts: 1682
- animal counts: 5491
- plant counts: 654
Sequence information
16S sequences
- @ref: 65257
- description: 16S rRNA gene sequence
- accession: LT594972
- database: nuccore
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Aeribacillus composti KCTC 33824 | GCA_007827555 | scaffold | ncbi | 1868734 |
66792 | Aeribacillus composti strain KCTC 33824 | 1868734.3 | wgs | patric | 1868734 |
66792 | Aeribacillus composti KCTC 33824 | 2802428823 | draft | img | 1868734 |
GC content
- @ref: 65257
- GC-content: 40.5
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | yes | 83.148 | yes |
flagellated | yes | 89.617 | no |
gram-positive | yes | 80.895 | no |
anaerobic | no | 96.54 | no |
aerobic | yes | 88.956 | yes |
halophile | no | 72.203 | no |
spore-forming | yes | 96.24 | yes |
glucose-util | yes | 85.628 | no |
thermophile | yes | 94.209 | yes |
glucose-ferment | no | 93.662 | no |
External links
@ref: 65257
culture collection no.: KCTC 33824, JCM 31580
literature
- topic: Phylogeny
- Pubmed-ID: 28984237
- title: Aeribacillus composti sp. nov., a thermophilic bacillus isolated from olive mill pomace compost.
- authors: Finore I, Gioiello A, Leone L, Orlando P, Romano I, Nicolaus B, Poli A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002391
- year: 2017
- mesh: Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, *Composting, DNA, Bacterial/genetics, Fatty Acids/chemistry, Italy, Nucleic Acid Hybridization, Olea/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
65257 | Ilaria Finore, Alessia Gioiello, Luigi Leone, Pierangelo Orlando, Ida Romano, Barbara Nicolaus, Annarita Poli | Aeribacillus composti sp. nov., a thermophilic bacillus isolated from olive mill pomace compost | 10.1099/ijsem.0.002391 | IJSEM 67: 4830-4835 2017 | 28984237 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 |