Strain identifier

BacDive ID: 158532

Type strain: Yes

Species: Streptomyces actinomycinicus

Strain Designation: RCU-197

Strain history: S. Tanasupawat; Chulalongkorn Univ., Thailand; RCU-197.

NCBI tax ID(s): 1695166 (species)

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General

@ref: 66446

BacDive-ID: 158532

DSM-Number: 111126

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, pigmented

description: Streptomyces actinomycinicus RCU-197 is an aerobe, spore-forming, mesophilic bacterium that builds branch substrate mycelia and aerial hyphae and has a yellow pigmentation.

NCBI tax id

  • NCBI tax id: 1695166
  • Matching level: species

strain history

@refhistory
66446<-JCM<-S. Tanasupawat; Chulalongkorn Univ., Thailand
67770S. Tanasupawat; Chulalongkorn Univ., Thailand; RCU-197.

doi: 10.13145/bacdive158532.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces actinomycinicus
  • full scientific name: Streptomyces actinomycinicus Tanasupawat et al. 2016

@ref: 66446

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces actinomycinicus

full scientific name: Streptomyces actinomycinicus Tanasupawat et al. 2016

strain designation: RCU-197

type strain: yes

Morphology

multicellular morphology

  • @ref: 65212
  • forms multicellular complex: yes
  • complex name: branch substrate mycelia and aerial hyphae
  • complex color: white to greenish-grey

pigmentation

  • @ref: 65212
  • production: yes
  • color: yellow

multimedia

  • @ref: 66446
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_111126.jpg
  • caption: Medium 252 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65212ISP2yes
65212ISP3yes
65212ISP4yes
65212ISP5yes
65212ISP7yes
65212ISP6yes
65212Nutrient agar (NA)yes
66446ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
66446STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
66446GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
65212positiveoptimum30-37
66446positivegrowth28
67770positivegrowth28

culture pH

  • @ref: 65212
  • ability: positive
  • type: optimum
  • pH: 5-9
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
65212aerobe
125438aerobe90.651

spore formation

  • @ref: 125438
  • spore formation: yes
  • confidence: 92.353

halophily

  • @ref: 65212
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-7 %(w/v)

observation

@refobservation
65212The N-acyl type of muramic acid is acetyl
65212Major menaquinones are MK-9(H 6 ) and MK-9(H 8 )
67770quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6521217057cellobiose+assimilation
6521262968cellulose-assimilation
6521216899D-mannitol+assimilation
6521216024D-mannose+assimilation
6521216988D-ribose+assimilation
6521265327D-xylose+assimilation
6521216813galactitol-assimilation
6521228757fructose+assimilation
652125291gelatin+hydrolysis
6521217234glucose+assimilation
6521217754glycerol+assimilation
6521230849L-arabinose+assimilation
6521217268myo-inositol+assimilation
6521217632nitrate-reduction
6521216634raffinose+assimilation
6521217814salicin+assimilation
65212skimmed milk+assimilation
6521228017starch+hydrolysis
6521217992sucrose-assimilation

enzymes

@refvalueactivityec
65212acid phosphatase+3.1.3.2
65212alkaline phosphatase+3.1.3.1
65212alpha-chymotrypsin+3.4.21.1
65212cystine arylamidase+3.4.11.3
65212esterase (C 4)+
65212esterase Lipase (C 8)+
65212alpha-galactosidase+3.2.1.22
65212beta-galactosidase+3.2.1.23
65212alpha-glucosidase+3.2.1.20
65212beta-glucosidase+3.2.1.21
65212N-acetyl-beta-glucosaminidase+3.2.1.52
65212trypsin+3.4.21.4
65212leucine arylamidase+3.4.11.1
65212naphthol-AS-BI-phosphohydrolase+
65212valine arylamidase+
65212alpha-mannosidase+3.2.1.24
65212alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65212C15:01.6
    65212C16:06.3
    65212C14:0 iso10
    65212C15:0 iso11.2
    65212C15:0 anteiso25.1
    65212C16:1 iso H1.6
    65212C16:0 iso25.8
    65212C17:0 iso2.8
    65212C17:0 anteiso6.1
    65212C16:1ω7c / C15:0 iso 2OH1.5
  • type of FA analysis: whole cell analysis
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
65212soilNong Jum Rung peat swamp forest, Rayong ProvinceThailandTHAAsia
66446Soil of a peat swamp forestRayong province, ThailandThailandTHAAsia12.6511101.547
67770Soil of a peat swamp forestRayong ProvinceThailandTHAAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 66446
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 65212
  • description: Streptomyces actinomycinicus gene for 16S ribosomal RNA, partial sequence, strain: RCU-197
  • accession: LC069046
  • database: nuccore

Genome sequences

  • @ref: 66792
  • description: Streptomyces actinomycinicus RCU-197
  • accession: GCA_016741775
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1695166

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes89.898no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.371yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes90.651yes
125438spore-formingspore-formingAbility to form endo- or exosporesyes92.353no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno96.5no
125438motile2+flagellatedAbility to perform flagellated movementno88.5no

External links

@ref: 66446

culture collection no.: JCM 30864, TISTR 2208, PCU 342, DSM 111126

straininfo link

  • @ref: 111319
  • straininfo: 398832

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26510888Streptomyces actinomycinicus sp. nov., isolated from soil of a peat swamp forest.Tanasupawat S, Phongsopitanun W, Suwanborirux K, Ohkuma M, Kudo TInt J Syst Evol Microbiol10.1099/ijsem.0.0007162015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Thailand, Vitamin K 2/analogs & derivatives/chemistry, *WetlandsGenetics
Phylogeny34196604Streptomyces musisoli sp. nov., an actinomycete isolated from soil.Duangupama T, Intaraudom C, Pittayakhajonwut P, Suriyachadkun C, Tadtong S, Sirirote P, Tanasupawat S, Thawai CInt J Syst Evol Microbiol10.1099/ijsem.0.0048572021Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Musa, Mycelium, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification, Thailand, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65212Somboon Tanasupawat, Wongsakorn Phongsopitanun, Khanit Suwanborirux, Moriya Ohkuma, Takuji KudoStreptomyces actinomycinicus sp. nov., isolated from soil of a peat swamp forest10.1099/ijsem.0.000716IJSEM 66: 290-295 201626510888
66446Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-111126Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 111126)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
111319Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398832.1
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695