Strain identifier

BacDive ID: 158528

Type strain: Yes

Species: Reinekea marina

NCBI tax ID(s): 1310421 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 8.1 (current version)

General

@ref: 65204

BacDive-ID: 158528

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Reinekea marina KACC 17315 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from seawater sample.

NCBI tax id

  • NCBI tax id: 1310421
  • Matching level: species

doi: 10.13145/bacdive158528.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Saccharospirillaceae
  • genus: Reinekea
  • species: Reinekea marina
  • full scientific name: Reinekea marina Kang et al. 2016

@ref: 65204

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Oceanospirillaceae

genus: Reinekea

species: Reinekea marina

type strain: yes

Morphology

cell morphology

  • @ref: 65204
  • gram stain: negative
  • cell length: 2.0-4.0 µm
  • cell width: 0.2-0.4 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 65204
  • colony size: 1.2 mm
  • colony color: beige
  • colony shape: circular
  • incubation period: 2 days
  • medium used: marine 2216

Culture and growth conditions

culture medium

@refnamegrowth
65204Nutrient agar (NA)no
65204Reasoner's 2A agar (R2A)no
65204tryptic soy agarno
65204blood agarno
65204MacConkey agarno
65204Marine agar (MA)yes

culture temp

@refgrowthtypetemperaturerange
65204positivegrowth4-42
65204positiveoptimum30mesophilic

culture pH

@refabilitytypepH
65204positivegrowth7.0-8.0
65204positiveoptimum7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 65204
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
65204NaClpositivegrowth0.5-3 %
65204NaClpositiveoptimum2 %
65204NaClnogrowth4 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6520430089acetate-oxidation
6520440585alpha-cyclodextrin-oxidation
6520417925alpha-D-glucose-oxidation
6520485146carboxymethylcellulose-hydrolysis
65204casein-hydrolysis
6520462968cellulose-hydrolysis
6520415824D-fructose-oxidation
6520412936D-galactose-oxidation
6520417634D-glucose+assimilation
6520416024D-mannose+assimilation
6520417924D-sorbitol-oxidation
6520423652dextrin-hydrolysis
6520423652dextrin-oxidation
6520424996lactate-oxidation
6520416991dna-hydrolysis
652044853esculin+hydrolysis
6520417234glucose-fermentation
6520428087glycogen-oxidation
6520417295L-phenylalanine+oxidation
6520417203L-proline-oxidation
6520425115malate+assimilation
6520417306maltose-oxidation
6520417306maltose+assimilation
6520428053melibiose-oxidation
6520417632nitrate+reduction
6520432032potassium gluconate+assimilation
6520417272propionate+oxidation
6520416634raffinose-oxidation
6520453258sodium citrate+assimilation
6520428017starch-hydrolysis
6520417992sucrose-oxidation
6520417748thymidine-oxidation
6520416704uridine-oxidation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6520428971ampicillinyesyes10 µg (disc)
6520417076streptomycinyesyes10 µg (disc)
6520418208penicillin gyesyes10 Unit (disc)
6520448923erythromycinyesyes15 µg (disc)
6520417698chloramphenicolyesyes30 µg (disc)
6520428077rifampicinyesyes30 µg (disc)
6520428001vancomycinyesyes30 µg (disc)
6520417833gentamicinyesyes10 µg (disc)
652046104kanamycinyesyes30 µg (disc)
6520427902tetracyclineyesyes30 µg (disc)

metabolite production

  • @ref: 65204
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

enzymes

@refvalueactivityec
65204cytochrome oxidase+1.9.3.1
65204catalase+1.11.1.6
65204alkaline phosphatase+3.1.3.1
65204leucine arylamidase+3.4.11.1
65204acid phosphatase+3.1.3.2
65204naphthol-AS-BI-phosphohydrolase+
65204N-acetyl-beta-glucosaminidase+3.2.1.52
65204esterase (C 4)-
65204esterase Lipase (C 8)-
65204lipase (C 14)-
65204valine arylamidase-
65204cystine arylamidase-3.4.11.3
65204trypsin-3.4.21.4
65204alpha-chymotrypsin-3.4.21.1
65204alpha-galactosidase-3.2.1.22
65204beta-glucuronidase-3.2.1.31
65204alpha-glucosidase-3.2.1.20
65204beta-glucosidase-3.2.1.21
65204alpha-mannosidase-3.2.1.24
65204alpha-fucosidase-3.2.1.51
65204arginine dihydrolase-3.5.3.6
65204urease-3.5.1.5
65204gelatinase-
65204beta-galactosidase+/-3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65204C10:0 3OH1.4
    65204C14:03.1
    65204C16:0 iso3.8
    65204C16:019
    65204C15:0 iso 3OH1.3
    65204C17?:1ω8c3.1
    65204C17:1ω6c2.4
    65204C17:01.9
    65204C16:1ω6c and/or C16:1ω7c43
    65204C18:1ω6c and/or C18:1ω7c15
  • type of FA analysis: whole cell analysis
  • incubation medium: marine 2216
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 65204
  • sample type: seawater sample
  • geographic location: Yellow Sea in Buan-Gun
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • latitude: 35.5816
  • longitude: 126.513

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_49645.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_425;96_10385;97_28019;98_35689;99_49645&stattab=map
  • Last taxonomy: Reinekea marina subclade
  • 16S sequence: KC690143
  • Sequence Identity:
  • Total samples: 539
  • soil counts: 7
  • aquatic counts: 504
  • animal counts: 26
  • plant counts: 2

Sequence information

16S sequences

  • @ref: 65204
  • description: 16S rRNA gene sequence
  • accession: KC690143
  • database: nuccore

GC content

  • @ref: 65204
  • GC-content: 46.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65204

culture collection no.: KACC 17315, CECT 8288, HME 8277

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26518601Reinekea marina sp. nov., isolated from seawater, and emended description of the genus Reinekea.Kang H, Kim H, Joung Y, Joh KInt J Syst Evol Microbiol10.1099/ijsem.0.0007202015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistryGenetics
Phylogeny33025185Reinekea thalattae sp. nov., a New Species of the Genus Reinekea Isolated from Surface Seawater in Sehwa Beach.Kim I, Chhetri G, Kim J, Kang M, Seo TCurr Microbiol10.1007/s00284-020-02224-82020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids, Gammaproteobacteria, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Seawater, Sequence Analysis, DNATranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65204Heeyoung Kang, Haneul Kim, Yochan Joung, Kiseong JohReinekea marina sp. nov., isolated from seawater, and emended description of the genus Reinekea10.1099/ijsem.0.000720IJSEM 66: 360-364 201626518601
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/