Strain identifier

BacDive ID: 158507

Type strain: Yes

Species: Segatella cerevisiae

Strain history: H. Kanda; Sapporo Breweries Ltd., Japan; SBC8034.

NCBI tax ID(s): 2053716 (species)

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General

@ref: 65183

BacDive-ID: 158507

DSM-Number: 100619

keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Segatella cerevisiae DSM 100619 is an obligate anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from brewery wastewater.

NCBI tax id

  • NCBI tax id: 2053716
  • Matching level: species

strain history

@refhistory
65183<- H. Kanda, Sapporo Breweries Ltd., Yaizu, Shizuoka, Japan; SBC8034 <- Y. Nakakita
67770H. Kanda; Sapporo Breweries Ltd., Japan; SBC8034.

doi: 10.13145/bacdive158507.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Segatella
  • species: Segatella cerevisiae
  • full scientific name: Segatella cerevisiae (Nakata et al. 2019) Hitch et al. 2023

  • synonyms

    • @ref: 20215
    • synonym: Prevotella cerevisiae

@ref: 65183

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella cerevisiae

full scientific name: Prevotella cerevisiae Nakata et al. 2019

type strain: yes

Morphology

cell morphology

  • @ref: 67892
  • gram stain: negative
  • cell length: 2-5 µm
  • cell width: 0.8 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 67892
  • colony size: 0.5-1 mm
  • colony color: white to off-white brown
  • colony shape: circular
  • incubation period: 5 days
  • medium used: MRS agar

Culture and growth conditions

culture medium

  • @ref: 65183
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
65183positivegrowth30mesophilic
67770positivegrowth30mesophilic

culture pH

  • @ref: 67892
  • ability: positive
  • type: optimum
  • pH: 5-7

Physiology and metabolism

oxygen tolerance

  • @ref: 67892
  • oxygen tolerance: obligate anaerobe

spore formation

  • @ref: 67892
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6789217234glucose+fermentation
6789217716lactose+fermentation
6789217306maltose+fermentation
6789237684mannose+fermentation
6789216634raffinose+fermentation
6789217814salicin+fermentation
6789222599arabinose+fermentation
6789217057cellobiose+fermentation
6789229864mannitol-fermentation
678926731melezitose-fermentation
6789226546rhamnose-fermentation
6789230911sorbitol-fermentation
6789227082trehalose-fermentation
6789218222xylose-fermentation
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6789235581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
67892leucyl glycin arylamidase+3.4.11.1
67892alanine arylamidase+3.4.11.2
67892alpha-arabinosidase+
67892serine arylamidase-
67892glutamate decarboxylase-4.1.1.15
67892urease-3.5.1.5
67892alkaline phosphatase+3.1.3.1
67892esterase (C 4)-
67892esterase Lipase (C 8)-
67892lipase (C 14)-
67892valine arylamidase-
67892cystine arylamidase-3.4.11.3
67892trypsin-3.4.21.4
67892alpha-chymotrypsin-3.4.21.1
67892alpha-galactosidase-3.2.1.22
67892beta-galactosidase+3.2.1.23
67892beta-glucosidase+3.2.1.21
67892alpha-glucosidase+3.2.1.20
67892N-acetyl-beta-glucosaminidase+3.2.1.52
67892acid phosphatase+3.1.3.2
67892alpha-fucosidase-3.2.1.51

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
65183--++-+++-+-+----+--+----+--+/--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
65183brewery wastewaterGunma prefectureJapanJPNAsia
67770Brewery wastewaterJapanJPNAsia
67892brewery wastewater

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Industrial wastewater
#Engineered#Waste#Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_57181.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_2396;96_25398;97_31832;98_40779;99_57181&stattab=map
  • Last taxonomy: Prevotellaceae
  • 16S sequence: LC331291
  • Sequence Identity:
  • Total samples: 884
  • soil counts: 62
  • aquatic counts: 303
  • animal counts: 435
  • plant counts: 84

Sequence information

16S sequences

  • @ref: 65183
  • description: Prevotella cerevisiae JCM 30867 gene for 16S rRNA, partial sequence
  • accession: LC331291
  • length: 1413
  • database: ena
  • NCBI tax ID: 2053716

Genome sequences

  • @ref: 66792
  • description: Prevotella cerevisiae DSM 100619
  • accession: GCA_024054555
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 2053716

GC content

  • @ref: 67770
  • GC-content: 45
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno96.843yes
gram-positiveno95.971yes
anaerobicyes98.33yes
aerobicno98.148yes
halophileno93.598no
spore-formingno95.752yes
motileno93.467yes
glucose-fermentyes79.744yes
thermophileno93.14no
glucose-utilyes86.574no

External links

@ref: 65183

culture collection no.: DSM 100619, JCM 30867, SBC 8034

straininfo link

  • @ref: 111302
  • straininfo: 405295

literature

  • topic: Phylogeny
  • Pubmed-ID: 30990398
  • title: Prevotella cerevisiae sp. nov., beer-spoilage obligate anaerobic bacteria isolated from brewery wastewater.
  • authors: Nakata H, Kanda H, Nakakita Y, Kaneko T, Tsuchiya Y
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003393
  • year: 2019
  • mesh: Bacteria, Anaerobic/genetics, Bacterial Typing Techniques, Base Composition, Beer/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Food Microbiology, Genes, Bacterial, *Phylogeny, Prevotella/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Waste Water/*microbiology
  • topic2: Biotechnology

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65183Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100619Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100619)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67892Hajime Nakata, Hajime Kanda, Yasukazu Nakakita, Takeshi Kaneko, Youichi TsuchiyaPrevotella cerevisiae sp. nov., beer-spoilage obligate anaerobic bacteria isolated from brewery wastewater10.1099/ijsem.0.003393IJSEM 69: 1789-1793 2019
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111302Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405295.1