Strain identifier

BacDive ID: 158439

Type strain: Yes

Species: Mycobacterium syngnathidarum

Strain Designation: 27335

Strain history: <- S. Sanchez, Univ. of Georgia, Athens, USA; 27335 <- A. Phillips, S. Sanchez, Univ. of Georgia, Athens, USA

NCBI tax ID(s): 1908205 (species)

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General

@ref: 65115

BacDive-ID: 158439

DSM-Number: 105112

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Mycobacterium syngnathidarum 27335 is a mesophilic bacterium that was isolated from Ovary of a pipefish .

NCBI tax id

  • NCBI tax id: 1908205
  • Matching level: species

strain history

  • @ref: 65115
  • history: <- S. Sanchez, Univ. of Georgia, Athens, USA; 27335 <- A. Phillips, S. Sanchez, Univ. of Georgia, Athens, USA

doi: 10.13145/bacdive158439.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium syngnathidarum
  • full scientific name: Mycobacterium syngnathidarum Fogelson et al. 2018

@ref: 65115

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium syngnathidarum

full scientific name: Mycobacterium syngnathidarum Fogelson et al. 2018

strain designation: 27335

type strain: yes

Morphology

multimedia

  • @ref: 65115
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_105112.jpg
  • caption: Medium 645 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65115MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
667005 % sheep's blood agaryes
667007H11 solid agar mediayes
66700Lowenstein-Jensen agaryes

culture temp

@refgrowthtypetemperaturerange
65115positivegrowth28mesophilic
66700positivegrowth30-37mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6670017268myo-inositol-growth
6670027082trehalose-growth
6670029864mannitol-growth
6670030911sorbitol-growth
6670062345L-rhamnose-growth
6670053426tween 80-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group IDis resistantresistance conc.
667003732clarithromycinyesyes0.5 µg/mL
667003732clarithromycinyesyes0.25 µg/mL
667002637Amikacinyesyes1 µg/mL
667002637Amikacinyesyes16 µg/mL
6670063607linezolidyesyes1 µg/mL
6670050845doxycyclineyesyes0.12 µg/mL
66700100241ciprofloxacinyesyes0.25 µg/mL
6670063611moxifloxacinyesyes0.25 µg/mL
6670045924trimethoprimyesyes1 µg/mL26
667009332sulfamethoxazoleyesyes19 µg/mL26
66700478164cefepimeyesyes>32 µg/mL
6670029007ceftriaxoneyesyes>64 µg/mL
6670050694minocyclineyesyes>8 µg/mL
6670048947clavulanic acidyesyes>32 µg/mL12
667002676amoxicillinyesyes>64 µg/mL12
66700149836tigecyclineyesyes0.25 µg/mL
6670028864tobramycinyesyes8 µg/mL
6670050845doxycyclineyesyes>16 µg/mL
6670063611moxifloxacinyesyes4 µg/mL
6670045924trimethoprimyes26yes4 µg/mL
667009332sulfamethoxazoleyes26yes76 µg/mL
66700471744imipenemyesyes32 µg/mL
66700209807cefoxitinyesyes>128 µg/mL

enzymes

@refvalueactivityec
66700cytochrome oxidase-1.9.3.1
66700arylsulfatase+3.1.6.1
66700catalase+1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment culture
65115Ovary of a pipefish (Syngnathoides baculeatus)South CarolinaUSAUSANorth America33.8361-81.1636
66700ovarian tissue from a clinically ill lined alligator pipefish (Syngnathoides biaculeatus) with gross and histologic lesions consistent with mycobacteriosis.Athens, GeorgiaUSAUSANorth AmericaMiddlebrook 7H11 media

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Urogenital tract#Ovary
#Host#Fishes

taxonmaps

  • @ref: 69479
  • File name: preview.99_515.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_115;97_219;98_419;99_515&stattab=map
  • Last taxonomy: Mycolicibacterium
  • 16S sequence: MG015886
  • Sequence Identity:
  • Total samples: 496
  • soil counts: 45
  • aquatic counts: 64
  • animal counts: 50
  • plant counts: 337

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
65115Mycobacterium sp. 27335 16S ribosomal RNA gene, partial sequenceMG0158861524ena1908205
65115Mycobacterium syngnathidarum strain 27335 NODE_66_length_1543_cov_54.1773, whole genome shotgun sequenceMLCL010000051543ena1908205
66700Mycobacterium sp. 27335 16S ribosomal RNA gene, partial sequenceMG015886.11524ena1908205

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium syngnathidarum 27335GCA_001942625contigncbi1908205
66792Mycobacterium syngnathidarum 273352862860994draftimg1908205

GC content

  • @ref: 66700
  • GC-content: 66.2
  • method: genome sequence analysis

External links

@ref: 65115

culture collection no.: DSM 105112, ATCC TSD-89

straininfo link

  • @ref: 111240
  • straininfo: 404094

literature

  • topic: Phylogeny
  • Pubmed-ID: 30272539
  • title: Mycobacterium syngnathidarum sp. nov., a rapidly growing mycobacterium identified in syngnathid fish.
  • authors: Fogelson SB, Camus AC, Lorenz W, Phillips A, Bartlett P, Sanchez S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002978
  • year: 2018
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Genes, Bacterial, Georgia, Mycobacterium/*classification/genetics/isolation & purification, Mycobacterium Infections/microbiology/*veterinary, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Smegmamorpha/*microbiology, South Carolina
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65115Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105112Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105112)
66700Susan B. Fogelson, Alvin C. Camus, Walt Lorenz, Ashley Phillips, Paula Bartlett, Susan SanchezMycobacterium syngnathidarum sp. nov., a rapidly growing mycobacterium identified in syngnathid fish10.1099/ijsem.0.002978IJSEM 68: 3696-3700 201830272539
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111240Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404094.1