Strain identifier

BacDive ID: 158436

Type strain: Yes

Species: Boudabousia tangfeifanii

Strain Designation: VUL4_3

Strain history: <- X. Meng, Chinese Center for Disease Control and Prevention, Beijing , China; VUL4_3

NCBI tax ID(s): 1912795 (species)

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General

@ref: 65112

BacDive-ID: 158436

DSM-Number: 103436

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Boudabousia tangfeifanii VUL4_3 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from faeces of vulture.

NCBI tax id

  • NCBI tax id: 1912795
  • Matching level: species

strain history

  • @ref: 65112
  • history: <- X. Meng, Chinese Center for Disease Control and Prevention, Beijing , China; VUL4_3

doi: 10.13145/bacdive158436.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Boudabousia
  • species: Boudabousia tangfeifanii
  • full scientific name: Boudabousia tangfeifanii (Meng et al. 2018) Yang et al. 2021

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces tangfeifanii

@ref: 65112

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Boudabousia

species: Boudabousia tangfeifanii

full scientific name: Boudabousia tangfeifanii (Meng et al. 2018) Yang et al. 2021

strain designation: VUL4_3

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
66727positive0.9-1.3 µm0.6-0.8 µmrod-shapedno
69480no90.457
69480positive100

colony morphology

  • @ref: 66727
  • colony size: 0.6-1 mm
  • colony color: greyish white
  • incubation period: 2 days
  • medium used: BHI-sheep-blood agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65112BBL ACTINOMYCES BROTH (DSMZ Medium 1029)yeshttps://mediadive.dsmz.de/medium/1029Name: BBL ACTINOMYCES BROTH (DSMZ Medium 1029) Composition: Infusion Broth 1.425 g/l Potassium Phosphate 0.855 g/l Dextrose 0.285 g/l Yeast extract 0.285 g/l Pancreatic digest of casein 0.228 g/l L-Cysteine HCl 0.057 g/l Starch 0.057 g/l Ammonium Sulfate 0.057 g/l Magnesium sulfate 0.0114 g/l Calcium Chloride 0.00057 g/l Distilled water
66727BHI-sheep-blood agaryes

culture temp

  • @ref: 65112
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
66727facultative anaerobe
69480anaerobe92.933

spore formation

@refspore formationconfidence
66727no
69480no100
69481no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
667276731melezitose-builds acid from
6672715443inulin-builds acid from
6672715963ribitol-builds acid from
6672716024D-mannose-builds acid from
6672716443D-tagatose-builds acid from
6672716634raffinose-builds acid from
6672716813galactitol-builds acid from
6672716899D-mannitol-builds acid from
6672716988D-ribose-builds acid from
6672717057cellobiose-builds acid from
6672717108D-arabinose-builds acid from
6672717113erythritol-builds acid from
6672717151xylitol-builds acid from
6672717266L-sorbose-builds acid from
6672717268myo-inositol-builds acid from
6672717814salicin-builds acid from
6672717924D-sorbitol-builds acid from
6672717992sucrose-builds acid from
6672718287L-fucose-builds acid from
6672718305arbutin-builds acid from
6672718333D-arabitol-builds acid from
6672718403L-arabitol-builds acid from
6672727082trehalose-builds acid from
6672727613amygdalin-builds acid from
6672728017starch-builds acid from
6672728053melibiose-builds acid from
6672728066gentiobiose-builds acid from
6672728087glycogen-builds acid from
6672728847D-fucose-builds acid from
6672730849L-arabinose-builds acid from
6672732032potassium gluconate-builds acid from
6672732528turanose-builds acid from
6672743943methyl alpha-D-mannoside-builds acid from
6672762318D-lyxose-builds acid from
6672762345L-rhamnose-builds acid from
6672765328L-xylose-builds acid from
6672774863methyl beta-D-xylopyranoside-builds acid from
66727320061methyl alpha-D-glucopyranoside-builds acid from
66727esculin ferric citrate-builds acid from
66727potassium 2-dehydro-D-gluconate-builds acid from
6672712936D-galactose+builds acid from
6672715824D-fructose+builds acid from
6672717306maltose+builds acid from
6672717634D-glucose+builds acid from
6672717716lactose+builds acid from
6672717754glycerol+builds acid from
6672759640N-acetylglucosamine+builds acid from
6672765327D-xylose+builds acid from
66727potassium 5-dehydro-D-gluconate+builds acid from
667275291gelatin+hydrolysis
6672717632nitrate+reduction

enzymes

@refvalueactivityec
66727alpha-galactosidase-3.2.1.22
66727alpha-glucosidase-3.2.1.20
66727alpha-mannosidase-3.2.1.24
66727arginine dihydrolase-3.5.3.6
66727beta-glucosidase-3.2.1.21
66727beta-glucuronidase-3.2.1.31
66727beta-mannosidase-3.2.1.25
66727cystine arylamidase-3.4.11.3
66727cytochrome oxidase-1.9.3.1
66727lipase-
66727pyrazinamidase-3.5.1.B15
66727pyroglutamic acid arylamidase-
66727pyrrolidonyl arylamidase-3.4.19.3
66727urease-3.5.1.5
66727acid phosphatase+3.1.3.2
66727alkaline phosphatase+3.1.3.1
66727alpha-fucosidase+3.2.1.51
66727beta-galactosidase+3.2.1.23
66727catalase+1.11.1.6
66727leucine arylamidase+3.4.11.1
66727N-acetyl-beta-glucosaminidase+3.2.1.52
66727naphthol-AS-BI-phosphohydrolase+
66727tripeptide aminopeptidase+3.4.11.4
66727trypsin+3.4.21.4
66727valine arylamidase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureisolation procedure
65112faeces of vultureQinghai provinceChinaCHNAsia
66727rectal swab sample of a wild vulture (Aegypius monachus)Tibetan autonomous region, Qinghai provinceChinaCHNAsiabrain-heart infusion-sheep blood agarThree Aegypius monachus vultures were captured from the Qinghai-Tibet plateau and their rectal swabs were separately dispensed in 2 ml sterile tubes containing Luria-Bertani medium (20 % glycerol added). During screening of the gut microbiota of Aegypius monachus, a novel strain, VUL4_3T, was isolated from one of the diluted samples (from the 10-1 and 10-2 dilutions) that were aerobically incubated in the presence of 5 % CO2 on brain-heart infusion-sheep blood agar.

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Birds

taxonmaps

  • @ref: 69479
  • File name: preview.99_6866.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_224;96_3229;97_3962;98_5055;99_6866&stattab=map
  • Last taxonomy: Boudabousia liubingyangii subclade
  • 16S sequence: KY039943
  • Sequence Identity:
  • Total samples: 908
  • soil counts: 25
  • aquatic counts: 38
  • animal counts: 843
  • plant counts: 2

Sequence information

16S sequences

  • @ref: 66727
  • description: Actinomyces sp. VUL4_3 16S ribosomal RNA gene, partial sequence
  • accession: KY039943
  • length: 1457
  • database: ena
  • NCBI tax ID: 1912795

Genome sequences

  • @ref: 66792
  • description: Boudabousia tangfeifanii VUL4_3
  • accession: GCA_001856685
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 1912795

GC content

@refGC-contentmethod
6511251.9
6672751.9genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno97.892yes
gram-positiveyes93.421yes
anaerobicno80.942yes
aerobicno97.623yes
halophileno82.331no
spore-formingno97.075no
thermophileno96.518no
glucose-utilyes87.567no
motileno93.693no
glucose-fermentyes81.389no

External links

@ref: 65112

culture collection no.: DSM 103436, CGMCC 4.7369

straininfo link

  • @ref: 111237
  • straininfo: 403390

literature

  • topic: Phylogeny
  • Pubmed-ID: 30351263
  • title: Actinomyces tangfeifanii sp. nov., isolated from the vulture Aegypius monachus.
  • authors: Meng X, Lai XH, Lu S, Liu S, Chen C, Zhou D, Yang J, Jin D, Xu J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003013
  • year: 2018
  • mesh: Actinomyces/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Falconiformes/*microbiology, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rectum/microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65112Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103436Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103436)
66727Xiangli Meng, Xin-He Lai, Shan Lu, Sha Liu, Cuixia Chen, Donggen Zhou, Jing Yang, Dong Jin, Jianguo XuActinomyces tangfeifanii sp. nov., isolated from the vulture Aegypius monachus10.1099/ijsem.0.003013IJSEM 68: 3701-3706 201830351263
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111237Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403390.1