Strain identifier

BacDive ID: 158380

Type strain: Yes

Species: Bifidobacterium callitrichidarum

Strain history: P. Mattarelli; Dept. of Agric. Sci., Bologna Univ., Italy; TRI 5.

NCBI tax ID(s): 2052941 (species)

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General

@ref: 65056

BacDive-ID: 158380

DSM-Number: 103152

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Bifidobacterium callitrichidarum DSM 103152 is an anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from Faeces of Saguinus imperator.

NCBI tax id

  • NCBI tax id: 2052941
  • Matching level: species

strain history

@refhistory
65056<- P. Mattarelli, Univ. Bologna, Italy <- M. Modesto and S. Michelini; TRI 5
67770P. Mattarelli; Dept. of Agric. Sci., Bologna Univ., Italy; TRI 5.

doi: 10.13145/bacdive158380.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Bifidobacteriales
  • family: Bifidobacteriaceae
  • genus: Bifidobacterium
  • species: Bifidobacterium callitrichidarum
  • full scientific name: Bifidobacterium callitrichidarum Modesto et al. 2018

@ref: 65056

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Bifidobacteriales

family: Bifidobacteriaceae

genus: Bifidobacterium

species: Bifidobacterium callitrichidarum

full scientific name: Bifidobacterium callitrichidarum Modesto et al. 2018

type strain: yes

Morphology

cell morphology

  • @ref: 65291
  • gram stain: positive
  • cell length: 5-8 µm
  • cell width: 1-1.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

@reftype of hemolysiscolony sizecolony colorcolony shapeincubation periodmedium used
65291gamma1-2 mmwhitecircular3 daysTPY agar
65291oval

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65056BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)yeshttps://mediadive.dsmz.de/medium/58Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water
65291TPY agaryes

culture temp

@refgrowthtypetemperaturerange
65056positivegrowth37mesophilic
65291positivegrowth22-48
65291nogrowth<20psychrophilic
65291nogrowth20psychrophilic
65291positiveoptimum45thermophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
65291positivegrowth4-7.5acidophile
65291positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65291anaerobe
65291microaerophile

observation

  • @ref: 65291
  • observation: asporogenous, F6PPK-positive and when growing in TPY broth are rods of various shapes forming a branched structure with ,Y' at both sides

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
65291168082-dehydro-D-gluconate-builds acid from
65291581435-dehydro-D-gluconate-builds acid from
6529115963ribitol-builds acid from
6529127613amygdalin+builds acid from
6529118305arbutin+builds acid from
6529117057cellobiose+builds acid from
6529118333D-arabitol-builds acid from
6529115824D-fructose+builds acid from
6529128847D-fucose-builds acid from
6529112936D-galactose+builds acid from
6529117634D-glucose+builds acid from
6529116024D-mannose-builds acid from
6529116443D-tagatose-builds acid from
6529116813galactitol-builds acid from
6529117113erythritol-builds acid from
652914853esculin+hydrolysis
6529128066gentiobiose+builds acid from
6529124265gluconate-builds acid from
6529117754glycerol-builds acid from
6529128087glycogen-builds acid from
6529130849L-arabinose+builds acid from
6529118403L-arabitol-builds acid from
6529118287L-fucose-builds acid from
6529117266L-sorbose-builds acid from
6529165328L-xylose-builds acid from
6529117716lactose+builds acid from
6529117306maltose+builds acid from
6529129864mannitol-builds acid from
6529128053melibiose+builds acid from
6529117632nitrate-reduction
6529116634raffinose+builds acid from
6529126546rhamnose-builds acid from
6529117814salicin+builds acid from
6529130911sorbitol-builds acid from
6529117992sucrose+builds acid from

metabolite production

  • @ref: 65291
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65291catalase-1.11.1.6
65291cytochrome oxidase-1.9.3.1
65291alpha-galactosidase+3.2.1.22
65291beta-galactosidase+3.2.1.23
65291alpha-glucosidase+3.2.1.20
65291beta-glucosidase+3.2.1.21
65291alpha-arabinosidase+3.2.1.55
65291arginine arylamidase+
65291proline-arylamidase+3.4.11.5
65291phenylalanine arylamidase+
65291leucine arylamidase+3.4.11.1
65291tyrosine arylamidase+
65291histidine arylamidase+
65291serine arylamidase+
65291urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedurehost species
65056Faeces of Saguinus imperatorBussolengo (Verona)ItalyITAEurope
65291fresh individual faecal sample of an adult subject of the emperor tamarin (Saguinus imperator), housed under semi-natural conditionsNatura Viva Garda Zoological Park, Bussolengo, VeronaItalyITAEurope2014-02TPY agarsupplemented with mupirocin (100 mg l-1)2-3 days371-2 g of faecal sample were serially diluted (tenfold) with peptone water (Merck) supplemented with cysteine hydrochloride (0.5 g l-1). Aliquots of 1 ml from each dilution (from 10-1 down to 10-9) were inoculated onto TPY agar. Plates were incubated under anaerobic conditions at 37°C for 48-72 h.
67770Feces of an adult subject of the emperor tamarin (Saguinus imperator), housed under semi-natural conditions in Natura Viva Garda Zoological ParkBussolengo, VeronaItalyITAEuropeSaguinus imperator

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Mammals#Primates

Safety information

risk assessment

  • @ref: 65056
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 65056
  • description: Bifidobacterium callitrichidarum strain TRI 5 16S ribosomal RNA gene, partial sequence
  • accession: KU298954
  • length: 1374
  • database: ena
  • NCBI tax ID: 2052941

GC content

@refGC-contentmethod
6505660.9
6529160.9high performance liquid chromatography (HPLC)

External links

@ref: 65056

culture collection no.: DSM 103152, JCM 31790, TRI 5

straininfo link

  • @ref: 111184
  • straininfo: 403270

literature

  • topic: Phylogeny
  • Pubmed-ID: 29116036
  • title: Bifidobacterium callitrichidarum sp. nov. from the faeces of the emperor tamarin (Saguinus imperator).
  • authors: Modesto M, Michelini S, Sansosti MC, De Filippo C, Cavalieri D, Qvirist L, Andlid T, Spiezio C, Sandri C, Pascarelli S, Sgorbati B, Mattarelli P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002472
  • year: 2017
  • mesh: Aldehyde-Lyases/chemistry, Animals, Bacterial Typing Techniques, Base Composition, Bifidobacterium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Multilocus Sequence Typing, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saguinus/*microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65056Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103152Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103152)
65291Monica Modesto, Samanta Michelini, Maria Cristina Sansosti, Carlotta De Filippo, Duccio Cavalieri, Linnea Qvirist, Thomas Andlid, Caterina Spiezio, Camillo Sandri, Stefano Pascarelli, Barbara Sgorbati, Paola MattarelliBifidobacterium callitrichidarum sp. nov. from the faeces of the emperor tamarin (Saguinus imperator)10.1099/ijsem.0.002472IJSEM 68: 141-148 201829116036
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
111184Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403270.1