Strain identifier

BacDive ID: 158364

Type strain: Yes

Species: Glycomyces anabasis

Strain history: <- Wenjun Li, Sun Yat-Sen Univ.

NCBI tax ID(s): 1840412 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 65040

BacDive-ID: 158364

DSM-Number: 46768

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Glycomyces anabasis DSM 46768 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from root of Anabasis aphylla L..

NCBI tax id

  • NCBI tax id: 1840412
  • Matching level: species

strain history

@refhistory
65040<- W.-J. Li, Yunnan Univ., Yunnan Inst. Microbiol. (YIM), Kunming, China; EGI 6500139 <- H.-F. Wang, Chinese Academy of Science
67770W.-J. Li EGI 6500139.
67771<- Wenjun Li, Sun Yat-Sen Univ.

doi: 10.13145/bacdive158364.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Glycomycetales
  • family: Glycomycetaceae
  • genus: Glycomyces
  • species: Glycomyces anabasis
  • full scientific name: Glycomyces anabasis Zhang et al. 2018

@ref: 65040

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Glycomycetaceae

genus: Glycomyces

species: Glycomyces anabasis

full scientific name: Glycomyces anabasis Zhang et al. 2018

type strain: yes

Morphology

cell morphology

  • @ref: 65543
  • gram stain: positive

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
65543yesaerial myceliumvivid yellowish pinkISP 2
65543yessubstrate myceliumstrong olive greenISP 2
65543yesaerial myceliumdeep olive greenISP 3
65543yessubstrate myceliumdeep olive greenISP 3
65543yessubstrate myceliumstrong olive greenISP 4
65543yesaerial myceliumstrong olive greenISP 4
65543yessubstrate myceliumyellowish whiteISP 5
65543yesaerial myceliumstrong olive greenISP 5
65543yessubstrate myceliumstrong olive greenNA
65543yesaerial myceliumstrong olive greenNA
65543yessubstrate myceliumstrong olive greenCzapek's agar
65543yesaerial myceliumdeep olive greenCzapek's agar
65543yessubstrate myceliumstrong olive greenpotato-dextrose-agar
65543yesaerial myceliumstrong olive greenpotato-dextrose-agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65040GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
65040ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
65543ISP 3yes
65543ISP 4yes
65543Czapek's agaryes
65543Nutrient agar (NA)yes
65543ISP 2yes
65543potato-dextrose-agaryes
65543ISP 5yes

culture temp

@refgrowthtypetemperaturerange
65040positivegrowth28mesophilic
65543positivegrowth15-40
65543positiveoptimum30mesophilic
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
65543positivegrowth6.0-10.0alkaliphile
65543positiveoptimum7.0-8.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65543aerobe
67771aerobe

spore formation

  • @ref: 65543
  • spore description: rod-like
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentrationhalophily level
65543NaClpositivegrowth0-10 %(w/v)
65543NaClpositiveoptimum4-6 %(w/v)moderately halophilic

observation

  • @ref: 67770
  • observation: quinones: MK-11, MK-11(H2)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
65543casein-hydrolysis
65543cellobiose-carbon source17057
65543cellulose+hydrolysis62968
65543D-fructose+carbon source15824
65543D-galactose+carbon source12936
65543D-glucose-carbon source17634
65543D-mannose-carbon source16024
65543D-ribose-carbon source16988
65543D-sorbitol-carbon source17924
65543xylitol-carbon source17151
65543D-xylose-carbon source65327
65543glycerol-carbon source17754
65543glycine-nitrogen source15428
65543L-arabinose-carbon source30849
65543L-arginine+nitrogen source16467
65543L-aspartate+nitrogen source29991
65543L-cystine+nitrogen source16283
65543L-glutamate+nitrogen source29985
65543L-histidine+nitrogen source15971
65543L-isoleucine+nitrogen source17191
65543L-lysine+nitrogen source18019
65543L-methionine+nitrogen source16643
65543L-phenylalanine-nitrogen source17295
65543L-rhamnose+carbon source62345
65543L-serine-nitrogen source17115
65543L-threonine+nitrogen source16857
65543L-tryptophan+nitrogen source16828
65543L-valine+nitrogen source16414
65543lactate+carbon source24996
65543melibiose-carbon source28053
65543myo-inositol-carbon source17268
65543nitrate-reduction17632
65543raffinose-carbon source16634
65543starch+hydrolysis28017
65543trehalose-carbon source27082
65543tween 20+hydrolysis53424
65543tween 40+hydrolysis53423
65543tween 60+hydrolysis53425
65543tween 80+hydrolysis53426

metabolite production

  • @ref: 65543
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
65543cytochrome oxidase+1.9.3.1
65543catalase+1.11.1.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65543C14:00.1
    65543C16:01.2
    65543C17:00.2
    65543C16:1 iso G1.9
    65543C17:1 anteiso A5.4
    65543C14:0 iso0.4
    65543C15:0 iso10
    65543C15:0 anteiso20.2
    65543C16:0 iso18.4
    65543C17:0 iso1.8
    65543C17:0 anteiso40.4
  • type of FA analysis: whole cell analysis
  • incubation medium: TSB
  • incubation temperature: 30
  • incubation time: 5
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationhost species
65040root of Anabasis aphylla L.Xinjiang ProvinceChinaCHNAsia
65543surface-sterilized roots of Anabasis aphyllaXinjiangChinaCHNAsiatapwater-yeast exract agarsupplemented with 3 %(w/v) NaCl3 weeks
67770Surface-sterilized roots of Anabasis aphylla L.Xinjiang, northwest ChinaChinaCHNAsiaAnabasis aphylla
67771From the surface-sterilized roots of Anabasis aphylla L.collected from XinjiangChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)

Sequence information

16S sequences

  • @ref: 65040
  • description: Glycomyces anabasis strain EGI 6500139 16S ribosomal RNA gene, partial sequence
  • accession: KX119421
  • length: 1553
  • database: ena
  • NCBI tax ID: 1840412

GC content

  • @ref: 67770
  • GC-content: 70.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65040

culture collection no.: DSM 46768, JCM 30088, KCTC 29495, EGI 6500139

straininfo link

  • @ref: 111169
  • straininfo: 401603

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny29485399Glycomyces anabasis sp. nov., a novel endophytic actinobacterium isolated from roots of Anabasis aphylla L.Zhang YG, Wang HF, Alkhalifah DHM, Xiao M, Zhou XK, Liu YH, Hozzein WN, Li WJInt J Syst Evol Microbiol10.1099/ijsem.0.0026682018Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Chenopodiaceae/*microbiology, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistryTranscriptome
Phylogeny34373934Glycomyces salinus sp. nov., an actinomycete isolated from a hypersaline habitat.Li R, Jiang GQ, Wang Y, Chen YG, Zhou EM, Tang SKArch Microbiol10.1007/s00203-021-02510-42021*Actinobacteria/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ecosystem, Fatty Acids/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65040Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-46768Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46768)
65543Yong-Guang Zhang, Hong-Fei Wang, Dalal Hussien M. Alkhalifah, Min Xiao, Xing-Kui Zhou, Yong-Hong Liu, Wael N. Hozzein and Wen-Jun LiGlycomyces anabasis sp. nov., a novel endophytic actinobacterium isolated from roots of Anabasis aphylla L.10.1099/ijsem.0.002668IJSEM 68: 1285-1290 201829485399
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
111169Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401603.1