Strain identifier

BacDive ID: 158355

Type strain: Yes

Species: Aestuariimonas insulae

Strain Designation: OITF-44

Strain history: <- Jung-Hoon YOON, Sungkyunkwan Univ.

NCBI tax ID(s): 2045267 (species)

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General

@ref: 65031

BacDive-ID: 158355

DSM-Number: 105891

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Aestuariimonas insulae OITF-44 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from tidal flat.

NCBI tax id

  • NCBI tax id: 2045267
  • Matching level: species

strain history

@refhistory
65031<- S. Park, Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea
67771<- Jung-Hoon YOON, Sungkyunkwan Univ.

doi: 10.13145/bacdive158355.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Aestuariimonas
  • species: Aestuariimonas insulae
  • full scientific name: Aestuariimonas insulae Park et al. 2018

@ref: 65031

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Aestuariimonas

species: Aestuariimonas insulae

full scientific name: Aestuariimonas insulae Park et al. 2018

strain designation: OITF-44

type strain: yes

Morphology

cell morphology

  • @ref: 65458
  • gram stain: negative
  • cell length: 0.5-10.0 µm
  • cell width: 0.2-0.6 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65458
  • colony size: 0.3-0.8 mm
  • colony color: vivid yellow
  • colony shape: circular
  • incubation period: 5 days
  • medium used: marine agar

pigmentation

@refproductionname
65458yesCarotenoid pigments
65458noFlexirubin-type

multimedia

  • @ref: 65031
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_105891.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65031MARINE BROTH (ROTH CP73) (DSMZ Medium 514f)yeshttps://mediadive.dsmz.de/medium/514fName: MARINE BROTH (ROTH CP73) (DSMZ Medium 514f) Composition: NaCl 19.4 g/l Agar 15.0 g/l MgCl2 8.8 g/l Peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
65458Marine agar (MA)yes

culture temp

@refgrowthtypetemperaturerange
65031positivegrowth25mesophilic
65458positiveoptimum25mesophilic
65458positivegrowth4-28
65458nogrowth30mesophilic
67771positivegrowth25mesophilic

culture pH

@refabilitytypepH
65458positiveoptimum7-8
65458positivegrowth6
65458nogrowth5.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65458aerobe
67771aerobe

halophily

@refsaltgrowthtested relationconcentration
65458NaClpositivegrowth0.5-5..0 %(w/v)
65458NaClpositiveoptimum2.0 %(w/v)

observation

@refobservation
65458Mg2+ ions are required for growth
65458predominant menaquinone is MK-6
65458major polar lipids are phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6545816150benzoate-carbon source
65458casein-hydrolysis
6545817057cellobiose-carbon source
6545815824D-fructose-carbon source
6545812936D-galactose-carbon source
6545817634D-glucose-carbon source
6545816024D-mannose-carbon source
6545865327D-xylose-carbon source
654584853esculin+hydrolysis
654585291gelatin-hydrolysis
6545817368hypoxanthine-hydrolysis
6545830849L-arabinose-carbon source
6545829985L-glutamate-carbon source
6545815589L-malate-carbon source
6545817895L-tyrosine+hydrolysis
6545817306maltose-carbon source
6545817632nitrate+reduction
6545817814salicin-carbon source
6545832954sodium acetate-carbon source
6545853258sodium citrate-carbon source
6545862965sodium formate-carbon source
6545850144sodium pyruvate-carbon source
6545828017starch-hydrolysis
6545830031succinate-carbon source
6545817992sucrose-carbon source
6545827082trehalose-carbon source
6545853426tween 80-hydrolysis
6545816199urea-hydrolysis
6545815318xanthine-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
6545818208penicillin gyesyes20 µg (disc)
654583542cephalothinyesyes30 µg (disc)
654583393carbenicillinyesyes100 µg (disc)
6545828368novobiocinyesyes5 µg (disc)
6545816869oleandomycinyesyes15 µg (disc)
6545827902tetracyclineyesyes15 Unit (disc)

metabolite production

  • @ref: 65458
  • Chebi-ID: 15138
  • metabolite: sulfide
  • production: no

enzymes

@refvalueactivityec
65458catalase+1.11.1.6
65458cytochrome oxidase+1.9.3.1
65458alkaline phosphatase+3.1.3.1
65458leucine arylamidase+3.4.11.1
65458valine arylamidase+
65458acid phosphatase+3.1.3.2
65458naphthol-AS-BI-phosphohydrolase+
65458N-acetyl-beta-glucosaminidase+3.2.1.52
65458esterase (C 4)-
65458esterase Lipase (C 8)-
65458lipase (C 14)-
65458cystine arylamidase-3.4.11.3
65458trypsin-3.4.21.4
65458alpha-chymotrypsin-3.4.21.1
65458alpha-galactosidase-3.2.1.22
65458beta-galactosidase-3.2.1.23
65458beta-glucuronidase-3.2.1.31
65458alpha-glucosidase-3.2.1.20
65458beta-glucosidase-3.2.1.21
65458alpha-mannosidase-3.2.1.24
65458alpha-fucosidase-3.2.1.51

fatty acid profile

  • @reffatty acidpercentage
    65458C15:1ω6c4.1
    65458C17:1ω6c3.1
    65458C14:0 iso2.3
    65458C15:0 iso16.7
    65458iso-C15:1 G*12.5
    65458iso-C16:1 H*11.9
    65458C16:0 iso5.5
    65458C15:0 anteiso3
    65458C17:1 iso ω9c2.5
    65458C17:0 2OH1.1
    65458C15:0 iso 3OH2.3
    65458C16:0 iso 3OH14.8
    65458C17:0 iso 3OH7.3
    65458C16:1ω7c and/or C16:1ω6c4.6
  • type of FA analysis: whole cell analysis
  • incubation medium: marine
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 3
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890
  • method/protocol: Sasser, 1990
  • cutoff value:
  • @reffatty acidpercentage
    65458C16:01.5
    65458C15:1ω6c3
    65458C17:1ω6c2.2
    65458C14:0 iso2.2
    65458C15:0 iso17
    65458iso-C15:1 G*13.3
    65458iso-C15:1 G*12.3
    65458C16:0 iso5.4
    65458C15:0 anteiso4.1
    65458anteiso-C15:1 A*1.1
    65458C17:1 iso ω9c2.1
    65458C17:0 2OH1.2
    65458C15:0 iso 3OH1.9
    65458C16:0 iso 3OH13.5
    65458C17:0 iso 3OH6.3
    65458C16:1ω7c and/or C16:1ω6c6.7
  • type of FA analysis: whole cell analysis
  • incubation medium: marine
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 5
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890
  • method/protocol: Sasser, 1990
  • cutoff value:
  • @reffatty acidpercentage
    65458C16:01.6
    65458C15:1ω6c3.4
    65458C17:1ω6c2
    65458C14:0 iso2.3
    65458C15:0 iso17.5
    65458iso-C15:1 G*14.1
    65458iso-C16:1 H*11.5
    65458C16:0 iso4.9
    65458C15:0 anteiso4.6
    65458anteiso-C15:1 A*1.4
    65458C17:1 iso ω9c1.9
    65458C17:0 2OH1.4
    65458C15:0 iso 3OH1.8
    65458C16:0 iso 3OH12.9
    65458C17:0 iso 3OH6.2
    65458C16:1ω7c and/or C16:1ω6c6.9
  • type of FA analysis: whole cell analysis
  • incubation medium: marine
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 7
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateenrichment cultureenrichment culture temperatureisolation procedure
65031tidal flatOido islandRepublic of KoreaKORAsia37.355126.689
65458a sample of tidal flat sediment from OidoYellow SeaRepublic of KoreaKORAsia37.3386126.6912016marine agar 221625isolated by the standard dilution plating technique
67771From mud flat soilOidoRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Tidal flat

Sequence information

16S sequences

  • @ref: 65031
  • description: Aestuariimonas insulae strain OITF-44 16S ribosomal RNA gene, partial sequence
  • accession: MG208086
  • length: 1448
  • database: ena
  • NCBI tax ID: 2045267

GC content

  • @ref: 65458
  • GC-content: 33.9
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65031

culture collection no.: DSM 105891, KACC 19569, KCTC 62197, NBRC 113118

straininfo link

  • @ref: 111160
  • straininfo: 401179

literature

  • topic: Phylogeny
  • Pubmed-ID: 29504931
  • title: Aestuariimonas insulae gen. nov., sp. nov., isolated from a tidal flat.
  • authors: Park S, Choi J, Park JM, Yoon JH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002684
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Geologic Sediments/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65031Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105891Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105891)
65458Sooyeon Park, Jeehyun Choi, Ji-Min Park, Jung-Hoon YoonAestuariimonas insulae gen. nov., sp. nov., isolated from a tidal flat10.1099/ijsem.0.002684IJSEM 68: 1365-1371 201829504931
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
111160Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401179.1