Strain identifier

BacDive ID: 158282

Type strain: Yes

Species: Izhakiella australiensis

Strain Designation: D4N98

Strain history: <- J. Mukan; School of Biotechnology, University of New South Wales, Randwick, Australia; D4N98

NCBI tax ID(s): 1926881 (species)

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General

@ref: 64942

BacDive-ID: 158282

DSM-Number: 105030

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, chemoheterotroph, mesophilic, Gram-negative, colony-forming

description: Izhakiella australiensis D4N98 is a facultative anaerobe, chemoheterotroph, mesophilic bacterium that forms circular colonies and was isolated from desert soil.

NCBI tax id

  • NCBI tax id: 1926881
  • Matching level: species

strain history

  • @ref: 64942
  • history: <- J. Mukan; School of Biotechnology, University of New South Wales, Randwick, Australia; D4N98

doi: 10.13145/bacdive158282.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Izhakiella
  • species: Izhakiella australiensis
  • full scientific name: Izhakiella australiensis Ji et al. 2017

@ref: 64942

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Izhakiella

species: Izhakiella australiensis

full scientific name: Izhakiella australiensis Ji et al. 2017

strain designation: D4N98

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
65404negativeno
69480yes98.285
69480negative99.941

colony morphology

  • @ref: 65404
  • colony color: colourless
  • colony shape: circular
  • incubation period: 1 day
  • medium used: LB agar

multimedia

  • @ref: 64942
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_105030.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64942TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
65404LB (Luria-Bertani) MEDIUMyes

culture temp

@refgrowthtypetemperaturerange
64942positivegrowth28mesophilic
65404positivegrowth10-37
65404nogrowth40thermophilic
65404positiveoptimum25-30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 65404
  • oxygen tolerance: facultative anaerobe

nutrition type

  • @ref: 65404
  • type: chemoheterotroph

spore formation

@refspore formationconfidence
69480no99.714
69481no100

observation

@refobservation
65404growth is observed on MacConkey agar, but no colour change is observed
65404dominant polar lipids are phospholipids including phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a neutral lipid and a glycolipid

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6540427613amygdalin-builds acid from
6540422599arabinose+builds acid from
6540418305arbutin-builds acid from
6540415963ribitol-builds acid from
6540417108D-arabinose-builds acid from
6540418333D-arabitol+builds acid from
6540417057cellobiose-builds acid from
6540415824D-fructose+builds acid from
6540428847D-fucose-builds acid from
6540412936D-galactose+builds acid from
6540417634D-glucose+assimilation
6540417634D-glucose+builds acid from
6540462318D-lyxose-builds acid from
6540416899D-mannitol+assimilation
6540416899D-mannitol+builds acid from
6540416899D-mannitol+fermentation
6540416024D-mannose+assimilation
6540416024D-mannose+builds acid from
6540416988D-ribose+builds acid from
6540417924D-sorbitol-builds acid from
6540416443D-tagatose+builds acid from
6540416813galactitol-builds acid from
6540417113erythritol+builds acid from
65404esculin ferric citrate-builds acid from
6540428066gentiobiose-builds acid from
6540417234glucose+fermentation
6540417754glycerol+builds acid from
6540428087glycogen-builds acid from
6540417268myo-inositol-builds acid from
6540417268myo-inositol-fermentation
6540415443inulin-builds acid from
6540416087isocitrate-carbon source
6540430849L-arabinose-fermentation
6540418403L-arabitol-builds acid from
6540418287L-fucose-builds acid from
6540462345L-rhamnose+assimilation
6540462345L-rhamnose+builds acid from
6540417266L-sorbose-builds acid from
6540417716lactose-builds acid from
6540417716lactose-fermentation
6540425115malate+assimilation
6540417306maltose-builds acid from
654046731melezitose-builds acid from
6540428053melibiose-builds acid from
65404320061methyl alpha-D-glucopyranoside-builds acid from
6540474863methyl beta-D-xylopyranoside-builds acid from
65404506227N-acetylglucosamine+assimilation
65404506227N-acetylglucosamine+builds acid from
6540417632nitrate-reduction
65404potassium 2-dehydro-D-gluconate-builds acid from
65404potassium 5-dehydro-D-gluconate-builds acid from
6540432032potassium gluconate-builds acid from
6540432032potassium gluconate+assimilation
6540416634raffinose-builds acid from
6540417814salicin-builds acid from
6540430911sorbitol-fermentation
6540428017starch-builds acid from
6540417992sucrose-builds acid from
6540417992sucrose-fermentation
6540415138sulfide-reduction
6540427082trehalose+builds acid from
6540427082trehalose+fermentation
6540432528turanose-builds acid from
6540417151xylitol-builds acid from
6540418222xylose-builds acid from
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6540416199ureano
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6540415688acetoin+
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
65404catalase+1.11.1.6
65404arginine dihydrolase+3.5.3.6
65404cystine arylamidase+3.4.11.3
65404acid phosphatase+3.1.3.2
65404naphthol-AS-BI-phosphohydrolase+
65404beta-galactosidase+3.2.1.23
65404lysine decarboxylase+4.1.1.18
65404ornithine decarboxylase-4.1.1.17
65404tryptophan deaminase-4.1.99.1
65404gelatinase-
65404cytochrome oxidase-1.9.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
64942+-+------+-++--+-----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
64942desert soilnear Glendambo (S 31° 1' 54'', E 135° 48' 24.48'')AustraliaAUSAustralia and Oceania-31.0317135.807
65404an Australian desert soilAustraliaAUSAustralia and Oceania

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Desert
#Environmental#Terrestrial#Soil
#Condition#Xerophilic

taxonmaps

  • @ref: 69479
  • File name: preview.99_7627.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_3552;97_4362;98_5581;99_7627&stattab=map
  • Last taxonomy: Izhakiella australiensis subclade
  • 16S sequence: KY744694
  • Sequence Identity:
  • Total samples: 166
  • soil counts: 13
  • aquatic counts: 13
  • animal counts: 116
  • plant counts: 24

Safety information

risk assessment

  • @ref: 64942
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64942
  • description: Izhakiella australiensis strain D4N98 16S ribosomal RNA gene, partial sequence
  • accession: KY744694
  • length: 1531
  • database: ena
  • NCBI tax ID: 1926881

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Izhakiella australiensis D4N98GCA_002006995scaffoldncbi1926881
66792Izhakiella australiensis D4N982791354900draftimg1926881

GC content

  • @ref: 64942
  • GC-content: 54.03
  • method: sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno68.55yes
gram-positiveno98.257no
anaerobicno97.443yes
aerobicno62.803yes
halophileno91.141no
spore-formingno94.158no
glucose-utilyes95.656yes
thermophileno99.106no
motileyes91.749no
glucose-fermentyes89.639yes

External links

@ref: 64942

culture collection no.: DSM 105030, LMG 30066

straininfo link

  • @ref: 111100
  • straininfo: 403945

literature

  • topic: Phylogeny
  • Pubmed-ID: 29130429
  • title: Izhakiella australiensis sp. nov. isolated from an Australian desert soil.
  • authors: Ji M, Tang S, Ferrari BC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002171
  • year: 2017
  • mesh: Australia, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Enterobacteriaceae/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Genes, Bacterial, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64942Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105030Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105030)
65404Mukan Ji, Sihui Tang, Belinda C. FerrariIzhakiella australiensis sp. nov. isolated from an Australian desert soil10.1099/ijsem.0.002171IJSEM 67: 4317-4322 201729130429
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111100Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403945.1