Strain identifier

BacDive ID: 158066

Type strain: No

Species: Pseudomonas mandelii

Strain Designation: 7HK4

Strain history: <- A. Krikstaponis, Institute of Biochemistry, Life Science Center, Vilnius, Lithuania;7HK4

NCBI tax ID(s): 75612 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 64640

BacDive-ID: 158066

DSM-Number: 107615

keywords: 16S sequence, Bacteria, mesophilic

description: Pseudomonas mandelii 7HK4 is a mesophilic bacterium that was isolated from soil of home yard.

NCBI tax id

  • NCBI tax id: 75612
  • Matching level: species

strain history

  • @ref: 64640
  • history: <- A. Krikstaponis, Institute of Biochemistry, Life Science Center, Vilnius, Lithuania;7HK4

doi: 10.13145/bacdive158066.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas mandelii
  • full scientific name: Pseudomonas mandelii Verhille et al. 1999

@ref: 64640

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Burkholderiales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas mandelii

full scientific name: Pseudomonas mandelii Verhille et al. 1999

strain designation: 7HK4

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64640MINERAL MEDIUM WITH VITAMINS (DSMZ Medium 462)yeshttps://mediadive.dsmz.de/medium/462Name: MINERAL MEDIUM (BRUNNER) WITH VITAMINS (DSMZ Medium 462) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Peptone 5.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Meat extract 3.0 g/l Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l Pyridoxamine 0.000125 g/l ZnSO4 x 7 H2O 0.0001 g/l Nicotinic acid 5e-05 g/l Vitamin B12 5e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l MnCl2 x 4 H2O 3e-05 g/l p-Aminobenzoate 2.5e-05 g/l Thiamine-HCl x 2 H2O 2.5e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Calcium pantothenate 1.25e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Biotin 5e-06 g/l Distilled water
64640REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 64640
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Isolation, sampling and environmental information

isolation

  • @ref: 64640
  • sample type: soil of home yard
  • country: Lithuania
  • origin.country: LTU
  • continent: Europe
  • latitude: 55.3614
  • longitude: 24.7443

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Garden
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_555.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_555&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: MH346031
  • Sequence Identity:
  • Total samples: 42314
  • soil counts: 16904
  • aquatic counts: 8731
  • animal counts: 11521
  • plant counts: 5158

Safety information

risk assessment

  • @ref: 64640
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64640
  • description: Pseudomonas mandelii strain 7HK4 16S ribosomal RNA gene, partial sequence
  • accession: MH346031
  • length: 1320
  • database: ena
  • NCBI tax ID: 75612

External links

@ref: 64640

culture collection no.: DSM 107615

straininfo link

  • @ref: 110910
  • straininfo: 407749

literature

  • topic: Metabolism
  • Pubmed-ID: 30321993
  • title: Biodegradation of 7-Hydroxycoumarin in Pseudomonas mandelii 7HK4 via ipso-Hydroxylation of 3-(2,4-Dihydroxyphenyl)-propionic Acid.
  • authors: Krikstaponis A, Meskys R
  • journal: Molecules
  • DOI: 10.3390/molecules23102613
  • year: 2018
  • mesh: Bacterial Proteins/genetics/metabolism, Cloning, Molecular, Hydrolases/genetics/metabolism, Hydroxylation, Mixed Function Oxygenases/genetics/metabolism, Multigene Family, *Operon, Oxygenases/genetics/metabolism, Phenylpropionates/*chemistry, Pseudomonas/genetics/*metabolism, Sequence Analysis, DNA, Umbelliferones/*chemistry
  • topic2: Enzymology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64640Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-107615Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 107615)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
110910Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407749.1