General

@ref: 64605

BacDive-ID: 158032

DSM-Number: 13256

keywords: 16S sequence, Bacteria, thermophilic

description: Thermosipho geolei SL31 is a thermophilic bacterium that was isolated from deep terrestrial petroleum reservoir.

NCBI tax id

• NCBI tax id: 117503
• Matching level: species

strain history

• @ref: 64605
• history: <- S. L'Haridon, UBO, Technopole Brest Iroise, France; SL31 {2018} <- S. L'Haridon {1998}

doi: 10.13145/bacdive158032.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/thermotogota
• domain: Bacteria
• phylum: Thermotogota
• class: Thermotogae
• order: Thermotogales
• family: Fervidobacteriaceae
• genus: Thermosipho
• species: Thermosipho geolei
• full scientific name: Thermosipho geolei L'Haridon et al. 2001

@ref: 64605

domain: Bacteria

phylum: Thermotogae

class: Thermotogae

order: Thermotogales

family: Fervidobacteriaceae

genus: Thermosipho

species: Thermosipho geolei

full scientific name: Thermosipho geolei L'Haridon et al. 2001

strain designation: SL31

type strain: yes

Culture and growth conditions

culture medium

• @ref: 64605
• name: PETROTOGA OLEARIA MEDIUM (DSMZ Medium 959)
• growth: yes
• link: https://mediadive.dsmz.de/medium/959
• composition: Name: PETROTOGA OLEARIA MEDIUM (DSMZ Medium 959) Composition: NaCl 15.0 g/l Maltose 3.5 g/l PIPES 3.4 g/l Na-acetate x 3 H2O 2.72 g/l Trypticase peptone 2.0 g/l Yeast extract 2.0 g/l NH4Cl 1.0 g/l Na2S x 9 H2O 0.5 g/l MgCl2 x 6 H2O 0.5 g/l K2HPO4 0.35 g/l KH2PO4 0.35 g/l KCl 0.2 g/l CaCl2 x 2 H2O 0.1 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

• @ref: 64605
• growth: positive
• type: growth
• temperature: 70
• range: thermophilic

Isolation, sampling and environmental information

isolation

• @ref: 64605
• sample type: deep terrestrial petroleum reservoir
• geographic location: Western Siberia
• country: Russia
• origin.country: RUS
• continent: Europe
• latitude: 61.1167
• longitude: 76.75

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_75008.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_93;96_2192;97_25788;98_52446;99_75008&stattab=map
• Last taxonomy: Thermosipho geolei subclade
• 16S sequence: AJ272022
• Sequence Identity:
• Total samples: 41
• soil counts: 2
• aquatic counts: 38
• animal counts: 1

Safety information

risk assessment

• @ref: 64605
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 64605
• description: Thermosipho sp. DSM 13256 partial 16S rRNA gene for 16S ribosomal RNA
• accession: AJ272022
• length: 1523
• database: ena
• NCBI tax ID: 117503

GC content

• @ref: 64605
• GC-content: 30.0
• method: thermal denaturation, midpoint method (Tm)

External links

@ref: 64605

culture collection no.: DSM 13256, JCM 10986

straininfo link

• @ref: 110877
• straininfo: 49448

literature

• topic: Phylogeny
• Pubmed-ID: 11491329
• title: Thermosipho geolei sp. nov., a thermophilic bacterium isolated from a continental petroleum reservoir in Western Siberia.
• authors: Haridon SL, Miroshnichenko ML, Hippe H, Fardeau ML, Bonch-Osmolovskaya E, Stackebrandt E, Jeanthon C
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/00207713-51-4-1327
• year: 2001
• mesh: Bacteria/*classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Drug Resistance, Microbial, Glucose/metabolism, Microscopy, Electron, Molecular Sequence Data, Nucleic Acid Hybridization, Petroleum/microbiology, Phylogeny, Siberia, Sulfur/metabolism
• topic2: Metabolism

Reference