Strain identifier

BacDive ID: 157758

Type strain: Yes

Species: Lawsonibacter asaccharolyticus

Strain Designation: 3BBH22

Strain history: <- DSM <- M Sakamoto, JCM 32166 <- M Sakamoto, RIKEN, Wako, Saitama, Japan, 3BBH22

NCBI tax ID(s): 2108523 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 64330

BacDive-ID: 157758

DSM-Number: 106493

keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Lawsonibacter asaccharolyticus 3BBH22 is an obligate anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from faeces of woman.

NCBI tax id

  • NCBI tax id: 2108523
  • Matching level: species

strain history

@refhistory
64330<- M. Sakamoto, JCM; JCM 32166 <- M. Sakamoto, RIKEN, Wako, Saitama, Japan; 3BBH22
67770M. Sakamoto; RIKEN BRC-JCM, Japan; 3BBH22.
67771<- DSM <- M Sakamoto, JCM 32166 <- M Sakamoto, RIKEN, Wako, Saitama, Japan, 3BBH22

doi: 10.13145/bacdive157758.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Oscillospiraceae
  • genus: Lawsonibacter
  • species: Lawsonibacter asaccharolyticus
  • full scientific name: Lawsonibacter asaccharolyticus Sakamoto et al. 2018

@ref: 64330

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Oscillospiraceae

genus: Lawsonibacter

species: Lawsonibacter asaccharolyticus

full scientific name: Lawsonibacter asaccharolyticus Sakamoto et al. 2018

strain designation: 3BBH22

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shape
66569positive2.4-20 µm0.8-1.5 µmrod-shaped
67771rod-shaped
67771positive

colony morphology

  • @ref: 66569
  • colony size: 0.5-1 mm
  • colony color: grey to off-white-grey
  • colony shape: circular
  • incubation period: 4 days
  • medium used: Brucella blood agar with haemin and menadione

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64330CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
66569Brucella blood agar with haemin and menadioneyes

culture temp

@refgrowthtypetemperaturerange
64330positivegrowth37mesophilic
66569positiveoptimum37mesophilic
66569positivegrowth30-45
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

culture pH

@refabilitytypepH
66569positiveoptimum7
66569positivegrowth6-8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66569obligate anaerobe
67771anaerobe

spore formation

@refspore formation
66569no
67771no

observation

@refobservation
66569sporulation-specific gene spo0A is present
66569No growth occurs on medium containing 20 % (w/v) bile.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6656916243quercetin-assimilation
6656916634raffinose-fermentation
6656937684mannose-fermentation
665694853esculin-hydrolysis
665695291gelatin-hydrolysis
6656917632nitrate-reduction
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6656916136hydrogen sulfideno
6656935581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
665696-phospho-beta-galactosidase-3.2.1.85
66569alanine arylamidase-3.4.11.2
66569alpha-arabinosidase-3.2.1.55
66569alpha-fucosidase-3.2.1.51
66569alpha-galactosidase-3.2.1.22
66569alpha-glucosidase-3.2.1.20
66569alpha-mannosidase-3.2.1.24
66569arginine dihydrolase-3.5.3.6
66569beta-galactosidase-3.2.1.23
66569beta-glucosidase-3.2.1.21
66569beta-glucuronidase-3.2.1.31
66569catalase-1.11.1.6
66569chymotrypsin-3.4.4.5
66569cystine arylamidase-3.4.11.3
66569glutamate decarboxylase-4.1.1.15
66569glutamyl-glutamate arylamidase-
66569glycin arylamidase-
66569histidine arylamidase-
66569leucyl glycin arylamidase-3.4.11.1
66569lipase-
66569N-acetyl-beta-glucosaminidase-3.2.1.52
66569phenylalanine arylamidase-
66569proline-arylamidase-3.4.11.5
66569pyroglutamic acid arylamidase-
66569trypsin-3.4.21.4
66569urease-3.5.1.5
66569valine arylamidase-
66569acid phosphatase+3.1.3.2
66569alkaline phosphatase+3.1.3.1
66569arginine arylamidase+
66569esterase+
66569esterase Lipase (C 8)+
66569leucine arylamidase+3.4.11.1
66569naphthol-AS-BI-phosphohydrolase+
66569serine arylamidase+
66569tyrosine arylamidase+
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageSD
    66569C18:1 w9c14.53.25
    66569C16:019.66.9
    66569C14:021.32.4
    66569C12:02.2
    66569C14:0 DMA2.6
    66569C18:0 DMA7.10.7
    66569C18:012.46.4
    66569C18:2 w6,9c12.24.5
    66569C16:0 DMA12.95.2
  • type of FA analysis: whole cell analysis
  • incubation medium: Brucella blood agar with haemin and menadione
  • agar/liquid: agar
  • incubation temperature: 37
  • incubation time: 4
  • software version: Sherlock 6.2B
  • library/peak naming table: BHIBLA
  • system: MIS MIDI
  • method/protocol: Miller 1982

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
64330-----------------------------
64330-----------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
64330faeces of womanJapanJPNAsia36.0239140.121
66569faecal sample of a healthy Japanese woman (41 years old)Brucella blood agar with haemin and menadione2-4 days37The fresh faecal sample was plated onto Brucella blood agar with haemin and menadione (BB; JCM Medium no. 677). After 2-4 days of incubation at 37°C under a H2/CO2/N2 (1 : 1 : 8, by vol.) gas mixture
67770Feces of a woman in the fortiesJapanJPNAsia
67771From a faecal sample of a healthy Japanese womanJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human#Female

taxonmaps

  • @ref: 69479
  • File name: preview.99_5624.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_91;96_299;97_3325;98_4191;99_5624&stattab=map
  • Last taxonomy: Eubacteriales
  • 16S sequence: LC371917
  • Sequence Identity:
  • Total samples: 72221
  • soil counts: 346
  • aquatic counts: 553
  • animal counts: 71245
  • plant counts: 77

Sequence information

16S sequences

  • @ref: 66569
  • description: Lawsonibacter asaccharolyticus 3BBH22 gene for 16S ribosomal RNA, partial sequence
  • accession: LC371917
  • length: 1481
  • database: ena
  • NCBI tax ID: 2108523

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lawsonibacter asaccharolyticus 3BBH222831418171draftimg2108523
67770Lawsonibacter asaccharolyticus 3BBH22GCA_003112755contigncbi2108523

GC content

@refGC-contentmethod
6656960.2-62.2genome sequence analysis
6777161.2high performance liquid chromatography (HPLC)

External links

@ref: 64330

culture collection no.: DSM 106493, JCM 32166, KCTC 15743

straininfo link

  • @ref: 110618
  • straininfo: 398672

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny29745868Lawsonibacter asaccharolyticus gen. nov., sp. nov., a butyrate-producing bacterium isolated from human faeces.Sakamoto M, Iino T, Yuki M, Ohkuma MInt J Syst Evol Microbiol10.1099/ijsem.0.0028002018Adult, Bacterial Typing Techniques, Base Composition, Butyrates/*metabolism, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Female, Humans, Japan, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAPathogenicity
Genetics29930067Draft Genome Sequence of Lawsonibacter asaccharolyticus JCM 32166(T), a Butyrate-Producing Bacterium, Isolated from Human Feces.Sakamoto M, Ikeyama N, Yuki M, Ohkuma MGenome Announc10.1128/genomeA.00563-182018Phylogeny

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64330Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-106493Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106493)
66569Mitsuo Sakamoto, Takao Iino, Masahiro Yuki, Moriya OhkumaLawsonibacter asaccharolyticus gen. nov., sp. nov., a butyrate-producing bacterium isolated from human faeces10.1099/ijsem.0.002800IJSEM 68: 2074-2081 201829745868
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
110618Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398672.1