Strain identifier
BacDive ID: 157758
Type strain:
Species: Lawsonibacter asaccharolyticus
Strain Designation: 3BBH22
Strain history: <- DSM <- M Sakamoto, JCM 32166 <- M Sakamoto, RIKEN, Wako, Saitama, Japan, 3BBH22
NCBI tax ID(s): 2108523 (species)
General
@ref: 64330
BacDive-ID: 157758
DSM-Number: 106493
keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, mesophilic, Gram-positive, rod-shaped, colony-forming
description: Lawsonibacter asaccharolyticus 3BBH22 is an obligate anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from faeces of woman.
NCBI tax id
- NCBI tax id: 2108523
- Matching level: species
strain history
@ref | history |
---|---|
64330 | <- M. Sakamoto, JCM; JCM 32166 <- M. Sakamoto, RIKEN; 3BBH22 |
67770 | M. Sakamoto; RIKEN BRC-JCM, Japan; 3BBH22. |
67771 | <- DSM <- M Sakamoto, JCM 32166 <- M Sakamoto, RIKEN, Wako, Saitama, Japan, 3BBH22 |
doi: 10.13145/bacdive157758.20241212.9.2
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Oscillospiraceae
- genus: Lawsonibacter
- species: Lawsonibacter asaccharolyticus
- full scientific name: Lawsonibacter asaccharolyticus Sakamoto et al. 2018
@ref: 64330
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Oscillospiraceae
genus: Lawsonibacter
species: Lawsonibacter asaccharolyticus
full scientific name: Lawsonibacter asaccharolyticus Sakamoto et al. 2018
strain designation: 3BBH22
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape |
---|---|---|---|---|
66569 | positive | 2.4-20 µm | 0.8-1.5 µm | rod-shaped |
67771 | rod-shaped | |||
67771 | positive |
colony morphology
- @ref: 66569
- colony size: 0.5-1 mm
- colony color: grey to off-white-grey
- colony shape: circular
- incubation period: 4 days
- medium used: Brucella blood agar with haemin and menadione
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
64330 | CHOPPED MEAT MEDIUM (DSMZ Medium 78) | yes | https://mediadive.dsmz.de/medium/78 | Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water |
66569 | Brucella blood agar with haemin and menadione | yes | ||
64330 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf |
culture temp
@ref | growth | type | temperature |
---|---|---|---|
64330 | positive | growth | 37 |
66569 | positive | optimum | 37 |
66569 | positive | growth | 30-45 |
67770 | positive | growth | 37 |
67771 | positive | growth | 37 |
culture pH
@ref | ability | type | pH |
---|---|---|---|
66569 | positive | optimum | 7 |
66569 | positive | growth | 6-8 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
66569 | obligate anaerobe |
67771 | anaerobe |
spore formation
@ref | spore formation |
---|---|
66569 | no |
67771 | no |
observation
@ref | observation |
---|---|
66569 | sporulation-specific gene spo0A is present |
66569 | No growth occurs on medium containing 20 % (w/v) bile. |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
66569 | 16243 | quercetin | - | assimilation |
66569 | 16634 | raffinose | - | fermentation |
66569 | 37684 | mannose | - | fermentation |
66569 | 4853 | esculin | - | hydrolysis |
66569 | 5291 | gelatin | - | hydrolysis |
66569 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
68380 | 16634 | raffinose | - | fermentation |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 16199 | urea | - | hydrolysis |
68380 | 17632 | nitrate | - | reduction |
68380 | 29985 | L-glutamate | - | degradation |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
66569 | 16136 | hydrogen sulfide | no |
66569 | 35581 | indole | no |
68380 | 35581 | indole | no |
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
66569 | 6-phospho-beta-galactosidase | - | 3.2.1.85 |
66569 | alanine arylamidase | - | 3.4.11.2 |
66569 | alpha-arabinosidase | - | 3.2.1.55 |
66569 | alpha-fucosidase | - | 3.2.1.51 |
66569 | alpha-galactosidase | - | 3.2.1.22 |
66569 | alpha-glucosidase | - | 3.2.1.20 |
66569 | alpha-mannosidase | - | 3.2.1.24 |
66569 | arginine dihydrolase | - | 3.5.3.6 |
66569 | beta-galactosidase | - | 3.2.1.23 |
66569 | beta-glucosidase | - | 3.2.1.21 |
66569 | beta-glucuronidase | - | 3.2.1.31 |
66569 | catalase | - | 1.11.1.6 |
66569 | chymotrypsin | - | 3.4.4.5 |
66569 | cystine arylamidase | - | 3.4.11.3 |
66569 | glutamate decarboxylase | - | 4.1.1.15 |
66569 | glutamyl-glutamate arylamidase | - | |
66569 | glycin arylamidase | - | |
66569 | histidine arylamidase | - | |
66569 | leucyl glycin arylamidase | - | 3.4.11.1 |
66569 | lipase | - | |
66569 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
66569 | phenylalanine arylamidase | - | |
66569 | proline-arylamidase | - | 3.4.11.5 |
66569 | pyroglutamic acid arylamidase | - | |
66569 | trypsin | - | 3.4.21.4 |
66569 | urease | - | 3.5.1.5 |
66569 | valine arylamidase | - | |
66569 | acid phosphatase | + | 3.1.3.2 |
66569 | alkaline phosphatase | + | 3.1.3.1 |
66569 | arginine arylamidase | + | |
66569 | esterase | + | |
66569 | esterase Lipase (C 8) | + | |
66569 | leucine arylamidase | + | 3.4.11.1 |
66569 | naphthol-AS-BI-phosphohydrolase | + | |
66569 | serine arylamidase | + | |
66569 | tyrosine arylamidase | + | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | serine arylamidase | - | |
68380 | histidine arylamidase | - | |
68380 | alanine arylamidase | - | 3.4.11.2 |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | phenylalanine arylamidase | - | |
68380 | leucyl glycin arylamidase | - | 3.4.11.1 |
68380 | proline-arylamidase | - | 3.4.11.5 |
68380 | L-arginine arylamidase | - | |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | alpha-glucosidase | - | 3.2.1.20 |
68380 | beta-galactosidase | - | 3.2.1.23 |
68380 | alpha-galactosidase | - | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | glycin arylamidase | - | |
68380 | leucine arylamidase | - | 3.4.11.1 |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | urease | - | 3.5.1.5 |
fatty acid profile
fatty acids
@ref fatty acid percentage SD 66569 C18:1 w9c 14.5 3.25 66569 C16:0 19.6 6.9 66569 C14:0 21.3 2.4 66569 C12:0 2.2 66569 C14:0 DMA 2.6 66569 C18:0 DMA 7.1 0.7 66569 C18:0 12.4 6.4 66569 C18:2 w6,9c 12.2 4.5 66569 C16:0 DMA 12.9 5.2 - type of FA analysis: whole cell analysis
- incubation medium: Brucella blood agar with haemin and menadione
- agar/liquid: agar
- incubation temperature: 37
- incubation time: 4
- software version: Sherlock 6.2B
- library/peak naming table: BHIBLA
- system: MIS MIDI
- method/protocol: Miller 1982
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
64330 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
64330 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture duration | enrichment culture temperature | isolation procedure |
---|---|---|---|---|---|---|---|---|---|---|
64330 | faeces of woman | Japan | JPN | Asia | 36.0239 | 140.121 | ||||
66569 | faecal sample of a healthy Japanese woman (41 years old) | Brucella blood agar with haemin and menadione | 2-4 days | 37 | The fresh faecal sample was plated onto Brucella blood agar with haemin and menadione (BB; JCM Medium no. 677). After 2-4 days of incubation at 37°C under a H2/CO2/N2 (1 : 1 : 8, by vol.) gas mixture | |||||
67770 | Feces of a woman in the forties | Japan | JPN | Asia | ||||||
67771 | From a faecal sample of a healthy Japanese woman | Japan | JPN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
#Host | #Human | #Female |
taxonmaps
- @ref: 69479
- File name: preview.99_5624.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_91;96_299;97_3325;98_4191;99_5624&stattab=map
- Last taxonomy: Eubacteriales
- 16S sequence: LC371917
- Sequence Identity:
- Total samples: 72221
- soil counts: 346
- aquatic counts: 553
- animal counts: 71245
- plant counts: 77
Sequence information
16S sequences
- @ref: 66569
- description: Lawsonibacter asaccharolyticus 3BBH22 gene for 16S ribosomal RNA, partial sequence
- accession: LC371917
- length: 1481
- database: nuccore
- NCBI tax ID: 2108523
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Lawsonibacter asaccharolyticus 3BBH22 | 2831418171 | draft | img | 2108523 |
67770 | Lawsonibacter asaccharolyticus 3BBH22 | GCA_003112755 | contig | ncbi | 2108523 |
GC content
@ref | GC-content | method |
---|---|---|
66569 | 60.2-62.2 | genome sequence analysis |
67771 | 61.2 | high performance liquid chromatography (HPLC) |
External links
@ref: 64330
culture collection no.: DSM 106493, JCM 32166, KCTC 15743
straininfo link
- @ref: 110618
- straininfo: 398672
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 29745868 | Lawsonibacter asaccharolyticus gen. nov., sp. nov., a butyrate-producing bacterium isolated from human faeces. | Sakamoto M, Iino T, Yuki M, Ohkuma M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002800 | 2018 | Adult, Bacterial Typing Techniques, Base Composition, Butyrates/*metabolism, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Female, Humans, Japan, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Pathogenicity |
Genetics | 29930067 | Draft Genome Sequence of Lawsonibacter asaccharolyticus JCM 32166(T), a Butyrate-Producing Bacterium, Isolated from Human Feces. | Sakamoto M, Ikeyama N, Yuki M, Ohkuma M | Genome Announc | 10.1128/genomeA.00563-18 | 2018 | Phylogeny |
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
64330 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-106493 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106493) | |||
66569 | Mitsuo Sakamoto, Takao Iino, Masahiro Yuki, Moriya Ohkuma | Lawsonibacter asaccharolyticus gen. nov., sp. nov., a butyrate-producing bacterium isolated from human faeces | 10.1099/ijsem.0.002800 | IJSEM 68: 2074-2081 2018 | 29745868 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
68380 | Automatically annotated from API rID32A | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
110618 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398672.1 |