Strain identifier
BacDive ID: 157756
Type strain:
Species: Ottowia konkukae
Strain Designation: SK3863
Strain history: <- S. K. Kim, Konkuk Univ., Seoul, Rep. of South Korea; SK3863 <- K.J. Yi
NCBI tax ID(s): 2338647 (species)
General
@ref: 64327
BacDive-ID: 157756
DSM-Number: 105395
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming
description: Ottowia konkukae SK3863 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from spoiled biji, soybean tofu residue.
NCBI tax id
- NCBI tax id: 2338647
- Matching level: species
strain history
- @ref: 64327
- history: <- S. K. Kim, Konkuk Univ., Seoul, Rep. of South Korea; SK3863 <- K.J. Yi
doi: 10.13145/bacdive157756.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Comamonadaceae
- genus: Ottowia
- species: Ottowia konkukae
- full scientific name: Ottowia konkukae Yi et al. 2018
@ref: 64327
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Clostridiales
family: Comamonadaceae
genus: Ottowia
species: Ottowia konkukae
full scientific name: Ottowia konkukae Yi et al. 2018
strain designation: SK3863
type strain: yes
Morphology
cell morphology
- @ref: 66724
- gram stain: negative
- cell length: 0.5-2.0 µm
- cell width: 0.5-1.0 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 66724
- colony size: 0.2-1.0 mm
- colony color: light yellow
- colony shape: circular
- incubation period: 3 days
- medium used: R2A medium
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
64327 | REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) | yes | https://mediadive.dsmz.de/medium/830c | Name: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
64327 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1; with strain-specific modifications) Composition: Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
66724 | MacConkey agar | no | ||
66724 | Reasoner's 2A agar (R2A) | yes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
64327 | positive | growth | 37 | mesophilic |
66724 | positive | optimum | 30 | mesophilic |
culture pH
- @ref: 66724
- ability: positive
- type: optimum
- pH: 6.5
Physiology and metabolism
oxygen tolerance
- @ref: 66724
- oxygen tolerance: aerobe
spore formation
- @ref: 66724
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
66724 | NaCl | positive | growth | 0-2 %(w/v) |
66724 | NaCl | positive | optimum | 0 %(w/v) |
observation
@ref | observation |
---|---|
66724 | Floc-forming ability can be observed in R2A broth at 25-37 C and at pH 6.0-8.5. |
66724 | Q-8 is the predominant quinone |
66724 | The polar lipids are phosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and five unidentified polar lipids. |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
66724 | 18276 | dihydrogen | - | aerobic catabolization |
66724 | 17632 | nitrate | + | anaerobic growth |
66724 | 15792 | malonate | - | assimilation |
66724 | 15971 | L-histidine | - | assimilation |
66724 | 17115 | L-serine | - | assimilation |
66724 | 17968 | butyrate | - | assimilation |
66724 | 18287 | L-fucose | - | assimilation |
66724 | 24265 | gluconate | - | assimilation |
66724 | 28087 | glycogen | - | assimilation |
66724 | 62345 | L-rhamnose | - | assimilation |
66724 | 72813 | exopolysaccharide | - | assimilation |
66724 | 16988 | D-ribose | + | assimilation |
66724 | 17203 | L-proline | + | assimilation |
66724 | 17272 | propionate | + | assimilation |
66724 | 17306 | maltose | + | assimilation |
66724 | 17924 | D-sorbitol | + | assimilation |
66724 | 17992 | sucrose | + | assimilation |
66724 | 30849 | L-arabinose | + | assimilation |
66724 | 16991 | dna | - | hydrolysis |
66724 | 28017 | starch | - | hydrolysis |
66724 | 53424 | tween 20 | - | hydrolysis |
66724 | 62968 | cellulose | - | hydrolysis |
66724 | casein | - | hydrolysis |
metabolite production
- @ref: 66724
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
66724 | alpha-chymotrypsin | - | 3.4.21.1 |
66724 | alpha-fucosidase | - | 3.2.1.51 |
66724 | alpha-galactosidase | - | 3.2.1.22 |
66724 | alpha-glucosidase | - | 3.2.1.20 |
66724 | alpha-mannosidase | - | 3.2.1.24 |
66724 | arginine dihydrolase | - | 3.5.3.6 |
66724 | beta-galactosidase | - | 3.2.1.23 |
66724 | beta-glucosidase | - | 3.2.1.21 |
66724 | beta-glucuronidase | - | 3.2.1.31 |
66724 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
66724 | trypsin | - | 3.4.21.4 |
66724 | urease | - | 3.5.1.5 |
66724 | acid phosphatase | + | 3.1.3.2 |
66724 | alkaline phosphatase | + | 3.1.3.1 |
66724 | beta-galactosidase | + | 3.2.1.23 |
66724 | catalase | + | 1.11.1.6 |
66724 | cystine arylamidase | + | 3.4.11.3 |
66724 | cytochrome oxidase | + | 1.9.3.1 |
66724 | esterase (C 4) | + | |
66724 | esterase Lipase (C 8) | + | |
66724 | leucine arylamidase | + | 3.4.11.1 |
66724 | lipase (C 14) | + | |
66724 | naphthol-AS-BI-phosphohydrolase | + | |
66724 | nitrate reductase | + | 1.7.99.4 |
66724 | thiosulfate reductase | + | 2.8.1.5 |
66724 | valine arylamidase | + |
fatty acid profile
fatty acids
@ref fatty acid percentage 66724 C17:1 anteiso A 0.5 66724 C16:0 3OH 0.6 66724 C12:0 3OH 1.8 66724 C18:1 w7c/C18:1 w6c 17.2 66724 C10:0 3OH 2.5 66724 C12:0 3.3 66724 C14:0 3.4 66724 C16:0 31.3 66724 C16:1 w6c/C16:1 w7c 33.7 66724 C17:0 cyclo 5.9 - type of FA analysis: whole cell analysis
- incubation medium: R2A
- incubation temperature: 25
- incubation time: 3
- software version: Sherlock 6.0
- library/peak naming table: TSBA60
- system: MIS MIDI
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture duration | enrichment culture temperature | isolation procedure |
---|---|---|---|---|---|---|---|---|---|
64327 | spoiled biji, soybean tofu residue | Seoul | Republic of Korea | KOR | Asia | ||||
66724 | from rotten biji (residue remaining after making tofu) | Republic of Korea | KOR | Asia | Reasoner's 2A (R2A) agar | 3 days | 25 | using the standard dilution-plating technique on Reasoner's 2A (R2A) agar at room temperature |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Engineered | #Food production | #Spoiled |
taxonmaps
- @ref: 69479
- File name: preview.99_174167.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_44601;97_84106;98_114027;99_174167&stattab=map
- Last taxonomy: Ottowia oryzae subclade
- 16S sequence: MG764241
- Sequence Identity:
- Total samples: 3960
- soil counts: 2514
- aquatic counts: 691
- animal counts: 260
- plant counts: 495
Sequence information
16S sequences
- @ref: 66724
- description: Ottowia konkukae 16S ribosomal RNA gene, partial sequence
- accession: MG764241
- length: 1459
- database: ena
- NCBI tax ID: 2338647
GC content
- @ref: 66724
- GC-content: 65.8
- method: high performance liquid chromatography (HPLC)
External links
@ref: 64327
culture collection no.: DSM 105395, KCCM 43236
straininfo link
- @ref: 110616
- straininfo: 402488
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 30222093 | Ottowia konkukae sp. nov., isolated from rotten biji (tofu residue). | Yi KJ, Im WT, Kim DW, Kim SK | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003009 | 2018 | Bacterial Typing Techniques, Base Composition, Comamonadaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Soy Foods/*microbiology, Ubiquinone/chemistry | Biotechnology |
Phylogeny | 35604929 | Ottowia testudinis sp. nov., isolated from the cloaca of a giant Asian pond turtle (Heosemys grandis). | Busse HJ, Kampfer P, Szostak MP, Spergser J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005348 | 2022 | Animals, Bacterial Typing Techniques, Base Composition, Cloaca, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Turtles | Transcriptome |
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
64327 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-105395 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105395) | |||
66724 | Kwon Jung Yi, Wan-Taek Im, Dong-Woon Kim and Soo-Ki Kim | Ottowia konkukae sp. nov., isolated from rotten biji (tofu residue) | 10.1099/ijsem.0.003009 | IJSEM 68: 3458-3462 2018 | 30222093 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
110616 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402488.1 |