Strain identifier

BacDive ID: 157739

Type strain: No

Species: Escherichia coli

Strain Designation: AW1.7

Strain history: <- M. G. Gänzle, Univ. Alberta, Edmonton, Canada; AW1.7 <- M. Aslam, AAFC Lacombe; AW1.7

NCBI tax ID(s): 562 (species)

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General

@ref: 64308

BacDive-ID: 157739

DSM-Number: 108612

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, human pathogen

description: Escherichia coli AW1.7 is a mesophilic human pathogen that was isolated from Beef.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 64308
  • history: <- M. G. Gänzle, Univ. Alberta, Edmonton, Canada; AW1.7 <- M. Aslam, AAFC Lacombe; AW1.7

doi: 10.13145/bacdive157739.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 64308

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: AW1.7

type strain: no

Culture and growth conditions

culture medium

  • @ref: 64308
  • name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/381
  • composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 64308
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
64308+-------+--++-++++-+-

Isolation, sampling and environmental information

isolation

  • @ref: 64308
  • sample type: Beef
  • geographic location: Beef Processing plant in Alberta
  • country: Canada
  • origin.country: CAN
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Meat
#Host#Mammals#Bovinae (Cow, Cattle)

Safety information

risk assessment

  • @ref: 64308
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64308
  • description: Escherichia coli strain AW1.7, whole genome shotgun sequencing project
  • accession: LDYJ00000000
  • length: 165
  • database: ena
  • NCBI tax ID: 562

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Escherichia coli AW1.7GCA_020388055completencbi562
66792Escherichia coli AW1.7GCA_016792445contigncbi562
66792Escherichia coli AW1.7GCA_001309455scaffoldncbi562
66792Escherichia coli AW1.72698536570draftimg562

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno59.258no
gram-positiveno98.035no
anaerobicno94.595no
aerobicyes86.336no
halophileno88.28no
spore-formingno93.517no
motileyes88.405no
thermophileno98.981yes
glucose-utilyes93.784no
glucose-fermentyes92.37no

External links

@ref: 64308

culture collection no.: DSM 108612

straininfo link

  • @ref: 110607
  • straininfo: 399534

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64308Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-108612Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 108612)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68368Automatically annotated from API 20E
110607Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399534.1