Strain identifier
BacDive ID: 157715
Type strain:
Species: Escherichia coli
Strain Designation: NVK1 4444-2, PEG-13-26-69
Strain history: <- M. Kresken, Antiinfectives Intelligence, Gesellschaft für klinisch-mikrobiologische Forschung und Kommunikation mbH
NCBI tax ID(s): 562 (species)
General
@ref: 64284
BacDive-ID: 157715
DSM-Number: 105378
keywords: Bacteria, mesophilic, antibiotic resistance, human pathogen
description: Escherichia coli NVK1 4444-2 is a mesophilic human pathogen that has multiple antibiotic resistances and was isolated from wound swab.
NCBI tax id
- NCBI tax id: 562
- Matching level: species
strain history
- @ref: 64284
- history: <- M. Kresken, Antiinfectives Intelligence, Gesellschaft für klinisch-mikrobiologische Forschung und Kommunikation mbH
doi: 10.13145/bacdive157715.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Escherichia
- species: Escherichia coli
- full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacillus coli
@ref: 64284
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Enterobacteriaceae
genus: Escherichia
species: Escherichia coli
full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919
strain designation: NVK1 4444-2, PEG-13-26-69
type strain: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
64284 | LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) | yes | https://mediadive.dsmz.de/medium/381 | Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water |
64284 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
culture temp
- @ref: 64284
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
antibiogram
- @ref: 64284
- medium: Mueller-Hinton Agar
- incubation temperature: 37
- oxygen condition: aerob
- Penicillin G: 0
- oxacillin: 0
- ampicillin: 0
- ticarcillin: 0
- mezlocillin: 6-8
- cefalotin: 0
- cefazolin: 0
- cefotaxime: 12
- aztreonam: 20
- imipenem: 34
- tetracycline: 0
- chloramphenicol: 30
- gentamycin: 24
- amikacin: 26
- vancomycin: 0
- erythromycin: 0
- lincomycin: 0
- ofloxacin: 26-28
- norfloxacin: 28-30
- colistin: 14
- pipemidic acid: 16
- nitrofurantoin: n.d.
- bacitracin: 0
- polymyxin b: 16
- kanamycin: 22-24
- neomycin: 20
- doxycycline: 10
- ceftriaxone: 12
- clindamycin: 0-6
- fosfomycin: 18
- moxifloxacin: 26-28
- linezolid: 0
- nystatin: 0
- quinupristin/dalfopristin: 0
- teicoplanin: 0
- piperacillin/tazobactam: 26
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68368 | 17632 | nitrate | + | reduction |
68368 | 30849 | L-arabinose | + | fermentation |
68368 | 27613 | amygdalin | - | fermentation |
68368 | 28053 | melibiose | + | fermentation |
68368 | 62345 | L-rhamnose | + | fermentation |
68368 | 30911 | sorbitol | - | fermentation |
68368 | 17268 | myo-inositol | - | fermentation |
68368 | 16899 | D-mannitol | + | fermentation |
68368 | 17634 | D-glucose | + | fermentation |
68368 | 5291 | gelatin | - | hydrolysis |
68368 | 27897 | tryptophan | + | energy source |
68368 | 16199 | urea | - | hydrolysis |
68368 | 16947 | citrate | - | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | + | degradation |
68368 | 29016 | arginine | - | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 16301 | nitrite | yes |
68368 | 15688 | acetoin | yes |
68368 | 35581 | indole | yes |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | + | |
68368 | 35581 | indole | + |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | cytochrome oxidase | - | 1.9.3.1 |
68368 | gelatinase | - | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | + | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX | NO2 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
64284 | + | - | + | - | - | - | - | - | + | + | - | + | + | - | - | + | +/- | + | - | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 64284
- sample type: wound swab
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Patient | #Swab |
#Host Body-Site | #Other | #Wound |
Safety information
risk assessment
- @ref: 64284
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 64284
culture collection no.: DSM 105378
straininfo link
- @ref: 110583
- straininfo: 406790
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
64284 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-105378 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105378) | |
68368 | Automatically annotated from API 20E | |||
110583 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID406790.1 |