Strain identifier

BacDive ID: 157715

Type strain: No

Species: Escherichia coli

Strain Designation: NVK1 4444-2, PEG-13-26-69

Strain history: <- M. Kresken, Antiinfectives Intelligence, Gesellschaft für klinisch-mikrobiologische Forschung und Kommunikation mbH

NCBI tax ID(s): 562 (species)

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General

@ref: 64284

BacDive-ID: 157715

DSM-Number: 105378

keywords: Bacteria, mesophilic, antibiotic resistance, human pathogen

description: Escherichia coli NVK1 4444-2 is a mesophilic human pathogen that has multiple antibiotic resistances and was isolated from wound swab.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 64284
  • history: <- M. Kresken, Antiinfectives Intelligence, Gesellschaft für klinisch-mikrobiologische Forschung und Kommunikation mbH

doi: 10.13145/bacdive157715.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 64284

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: NVK1 4444-2, PEG-13-26-69

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64284LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
64284TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 64284
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

antibiogram

  • @ref: 64284
  • medium: Mueller-Hinton Agar
  • incubation temperature: 37
  • oxygen condition: aerob
  • Penicillin G: 0
  • oxacillin: 0
  • ampicillin: 0
  • ticarcillin: 0
  • mezlocillin: 6-8
  • cefalotin: 0
  • cefazolin: 0
  • cefotaxime: 12
  • aztreonam: 20
  • imipenem: 34
  • tetracycline: 0
  • chloramphenicol: 30
  • gentamycin: 24
  • amikacin: 26
  • vancomycin: 0
  • erythromycin: 0
  • lincomycin: 0
  • ofloxacin: 26-28
  • norfloxacin: 28-30
  • colistin: 14
  • pipemidic acid: 16
  • nitrofurantoin: n.d.
  • bacitracin: 0
  • polymyxin b: 16
  • kanamycin: 22-24
  • neomycin: 20
  • doxycycline: 10
  • ceftriaxone: 12
  • clindamycin: 0-6
  • fosfomycin: 18
  • moxifloxacin: 26-28
  • linezolid: 0
  • nystatin: 0
  • quinupristin/dalfopristin: 0
  • teicoplanin: 0
  • piperacillin/tazobactam: 26

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836817632nitrate+reduction
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836816301nitriteyes
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole+

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOXNO2
64284+-+-----++-++--++/-+-+-+

Isolation, sampling and environmental information

isolation

  • @ref: 64284
  • sample type: wound swab
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Swab
#Host Body-Site#Other#Wound

Safety information

risk assessment

  • @ref: 64284
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 64284

culture collection no.: DSM 105378

straininfo link

  • @ref: 110583
  • straininfo: 406790

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64284Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105378Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105378)
68368Automatically annotated from API 20E
110583Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406790.1