Strain identifier
BacDive ID: 157587
Type strain: 
Species: Gilliamella apis
Strain Designation: NO3
Strain history: <- J. Ludvigsen, Norwegian Univ. of Science and Technol., Trondheim, Norway; NO3
NCBI tax ID(s): 1970738 (species)
version 8.1 (current version)
General
@ref: 64156
BacDive-ID: 157587
DSM-Number: 105629
keywords: genome sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped
description: Gilliamella apis NO3 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from honeybee gut.
NCBI tax id
- NCBI tax id: 1970738
- Matching level: species
strain history
- @ref: 64156
- history: <- J. Ludvigsen, Norwegian Univ. of Science and Technol., Trondheim, Norway; NO3
doi: 10.13145/bacdive157587.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Orbales
- family: Orbaceae
- genus: Gilliamella
- species: Gilliamella apis
- full scientific name: Gilliamella apis Ludvigsen et al. 2018
@ref: 64156
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Orbales
family: Orbaceae
genus: Gilliamella
species: Gilliamella apis
full scientific name: Gilliamella apis Ludvigsen et al. 2018
strain designation: NO3
type strain: yes
Morphology
cell morphology
- @ref: 65477
- gram stain: negative
- cell length: 1-0.25 µm
- cell shape: rod-shaped
colony morphology
| @ref | colony size | incubation period | medium used |
|---|---|---|---|
| 65477 | <1 mm | 2 days | Trypticase soy agar + 5% horse blood |
| 65477 | <1 mm | HIA + 5 % horse blood |
multimedia
- @ref: 64156
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_105629.jpg
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 64156 | BHI MEDIUM (DSMZ Medium 215) | yes | https://mediadive.dsmz.de/medium/215 | Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water |
| 65477 | Trypticase soy agar | yes | microaerophilic atmosphere enriched with CO2 at 36°C, after 48-72 h at pH 7.0-7.5 | |
| 65477 | HIA | yes | ||
| 65477 | Trypticase soy agar + 5% horse blood | yes | ||
| 65477 | HIA + 5 % horse blood | yes |
culture temp
- @ref: 64156
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 65477
- oxygen tolerance: anaerobe
observation
| @ref | observation |
|---|---|
| 65477 | harbours the genes xylA, xylB, rhaA, rhaB and rhaD for xylene and rhamnose catabolism |
| 65477 | harbours the Tet H determinant that confers resistance towards tetracycline |
| 65477 | polar lipids are phosphoaminoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 65477 | 15824 | D-fructose | + | fermentation |
| 65477 | 17634 | D-glucose | + | fermentation |
| 65477 | 17992 | sucrose | + | fermentation |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 65477 | 15688 | acetoin | yes |
| 65477 | 16899 | D-mannitol | no |
| 65477 | 16024 | D-mannose | no |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 65477 | catalase | - | 1.11.1.6 |
| 65477 | cytochrome oxidase | - | 1.9.3.1 |
| 65477 | alkaline phosphatase | + | 3.1.3.1 |
| 65477 | beta-galactosidase | - | 3.2.1.23 |
| 65477 | gamma-glutamyltransferase | - | 2.3.2.2 |
| 65477 | urease | - | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | isolation procedure |
|---|---|---|---|---|---|---|---|---|
| 64156 | honeybee gut | Aas | Norway | NOR | Europe | 59.6644 | 10.7944 | |
| 65477 | homogenized guts collected from honeybees | Ås | Norway | NOR | Europe | isolated after 48 h of incubation at 37°C, in an enhanced CO2 atmosphere (BD GasPak EZ CO2 Container system, Becton Dickinson; CO2 2.5 %), on tryptic soy agar (TSA) supplemented with 5 % horse blood and 12µg tetracycline ml^-1 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Arthropoda | #Insecta |
| #Host Body-Site | #Gastrointestinal tract |
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Gilliamella apis NO3 | GCA_002142155 | contig | ncbi | 1970738 |
| 66792 | Gilliamella apis NO3 | 2834098943 | draft | img | 1970738 |
External links
@ref: 64156
culture collection no.: DSM 105629, LMG 30293
straininfo link
- @ref: 110456
- straininfo: 402083
literature
- topic: Phylogeny
- Pubmed-ID: 29624166
- title: Addressing the diversity of the honeybee gut symbiont Gilliamella: description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera).
- authors: Ludvigsen J, Porcellato D, Amdam GV, Rudi K
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002749
- year: 2018
- mesh: Animals, Bacterial Typing Techniques, Base Composition, Bees/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, Gastrointestinal Tract/*microbiology, Norway, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Symbiosis, Ubiquinone/chemistry
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 64156 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-105629 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105629) | |||
| 65477 | Jane Ludvigsen, Davide Porcellato, Gro V. Amdam, Knut Rudi | Addressing the diversity of the honeybee gut symbiont Gilliamella: description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera) | 10.1099/ijsem.0.002749 | IJSEM 68: 1762-1770 2018 | 29624166 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 110456 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402083.1 |