Strain identifier

BacDive ID: 157075

Type strain: Yes

Species: Streptococcus azizii

Strain Designation: 12-5202

Strain history: <- P. L. Shewmaker; 12-5202

NCBI tax ID(s): 1579424 (species)

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General

@ref: 66488

BacDive-ID: 157075

DSM-Number: 103678

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, animal pathogen

description: Streptococcus azizii 12-5202 is a microaerophile, mesophilic, Gram-positive animal pathogen that was isolated from Strains 12-5202 Tbrain swab of a 29-day-old male mouse found runted and hunched with neurologic signs.

NCBI tax id

  • NCBI tax id: 1579424
  • Matching level: species

strain history

  • @ref: 66488
  • history: <- P. L. Shewmaker; 12-5202

doi: 10.13145/bacdive157075.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus azizii
  • full scientific name: Streptococcus azizii Shewmaker et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus azizii

@ref: 66488

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus azizii

full scientific name: Streptococcus azizii Shewmaker et al. 2017

strain designation: 12-5202

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
65236positivecoccus-shapedno
125438no91.979

colony morphology

  • @ref: 65236
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • colony size: 1 mm
  • colony color: grey-white
  • incubation period: 1 day
  • medium used: TSA agar

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
65236TSA agaryessupplemented with 5 % sheep blood
65236heart infusion brothyes
65236Columbia blood agaryes
66488COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
66488TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yesName: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/92

culture temp

@refgrowthtypetemperature
63577positivegrowth37
65236positivegrowth10-45
66488positivegrowth37

Physiology and metabolism

oxygen tolerance

  • @ref: 63577
  • oxygen tolerance: microaerophile

spore formation

  • @ref: 125438
  • spore formation: no
  • confidence: 90.24

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6523622599arabinose-builds acid from
6523629016arginine-hydrolysis
6523623456cyclodextrin-builds acid from
6523618333D-arabitol-builds acid from
6523617924D-sorbitol-builds acid from
652364853esculin+hydrolysis
6523617754glycerol-builds acid from
6523615978glycerol 3-phosphate-builds acid from
6523628087glycogen-builds acid from
65236606565hippurate+hydrolysis
6523615443inulin+builds acid from
6523617716lactose+builds acid from
6523617306maltose+builds acid from
6523629864mannitol+builds acid from
652366731melezitose-builds acid from
6523628053melibiose+/-builds acid from
65236320061methyl alpha-D-glucopyranoside-builds acid from
65236320055methyl beta-D-glucopyranoside+builds acid from
6523627941pullulan+builds acid from
6523616634raffinose+/-builds acid from
6523633942ribose+builds acid from
6523630911sorbitol-builds acid from
6523627922sorbose-builds acid from
6523628017starch-hydrolysis
6523617992sucrose+builds acid from
6523627082trehalose+builds acid from
6523616199urea-hydrolysis
6523618222xylose-builds acid from

metabolite production

  • @ref: 65236
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

enzymes

@refvalueactivityec
65236catalase-1.11.1.6
65236beta-glucosidase+3.2.1.21
65236beta-galactosidase+3.2.1.23
65236beta-glucuronidase+3.2.1.31
65236tripeptide aminopeptidase+3.4.11.4
65236pyroglutamic acid arylamidase+
65236glycyl tryptophan arylamidase+
65236beta-mannosidase+3.2.1.25
65236arginine dihydrolase-3.5.3.6
65236alpha-galactosidase-3.2.1.22
65236alkaline phosphatase-3.1.3.1
65236N-acetyl-beta-glucosaminidase-3.2.1.52
65236urease-3.5.1.5
65236pyrrolidonyl arylamidase+3.4.19.3

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
65236Strains 12-5202 Tbrain swab of a 29-day-old male mouse found runted and hunched with neurologic signsCenters for Disease Control and Prevention (CDC) Streptococcus laboratoryUSAUSANorth America
66488brain swab of a 29-day-old male naïve weanling mouse found runted and hunched with neurologic signsNew York, New York Weill Cornell MedicineUSAUSANorth America40.7306-73.9352

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body-Site#Oral cavity and airways

Safety information

risk assessment

  • @ref: 66488
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 66488
  • description: Streptococcus azizii strain 12-5202 16S ribosomal RNA gene, partial sequence
  • accession: KM609118
  • length: 1538
  • database: nuccore
  • NCBI tax ID: 1579424

Genome sequences

  • @ref: 66792
  • description: Streptococcus azizii 12-5202
  • accession: GCA_001984715
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1579424

GC content

@refGC-contentmethod
6523642.76genome sequence analysis
6648842.76sequence analysis

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes87.385yes
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no90.969yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.478yes
125438spore-formingspore-formingAbility to form endo- or exosporesno90.24no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno94.5yes
125438motile2+flagellatedAbility to perform flagellated movementno91.979yes

External links

@ref: 66488

culture collection no.: CCUG 69378, DSM 103678

straininfo link

  • @ref: 110204
  • straininfo: 396538

literature

  • topic: Phylogeny
  • Pubmed-ID: 29022542
  • title: Streptococcus azizii sp. nov., isolated from naive weanling mice.
  • authors: Shewmaker PL, Whitney AM, Gulvik CA, Lipman NS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002407
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Female, Genes, Bacterial, Male, Meningoencephalitis/*microbiology, Mice/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptococcus/*classification/genetics/isolation & purification
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
63577Curators of the CCUGhttps://www.ccug.se/strain?id=69378Culture Collection University of Gothenburg (CCUG) (CCUG 69378)
65236Patricia Lynn Shewmaker, Anne M. Whitney, Christopher A. Gulvik, Neil S. LipmanStreptococcus azizii sp. nov., isolated from naïve weanling mice10.1099/ijsem.0.002407IJSEM 67: 5032-5037 201729022542
66488Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103678Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103678)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
110204Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID396538.1
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695