Strain identifier

BacDive ID: 157

Type strain: Yes

Species: Actinomyces viscosus

Strain history: CIP <- 1988, NCTC <- 1974, ATCC <- A. Howel, NIH: strain Jr. T-6, Odontomyces viscosus <- H.V. Jordan

NCBI tax ID(s): 1656 (species)

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General

@ref: 10957

BacDive-ID: 157

DSM-Number: 43327

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, animal pathogen

description: Actinomyces viscosus DSM 43327 is an anaerobe, mesophilic animal pathogen that was isolated from periodontal disease in hamsters.

NCBI tax id

  • NCBI tax id: 1656
  • Matching level: species

strain history

@refhistory
10957<- M.A. Gerencser, WVU 745 <- ATCC <- A. Howell, NIH T-6 (Odontomyces viscosus) <- H.V. Jordan
67770N. Maeda TPR-12 <-- ATCC 15987 <-- A. Howell, Jr. T-6 <-- H. V. Jordan.
123875CIP <- 1988, NCTC <- 1974, ATCC <- A. Howel, NIH: strain Jr. T-6, Odontomyces viscosus <- H.V. Jordan

doi: 10.13145/bacdive157.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Actinomyces
  • species: Actinomyces viscosus
  • full scientific name: Actinomyces viscosus (Howell et al. 1965) Georg et al. 1969 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Odontomyces viscosus

@ref: 10957

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Actinomyces

species: Actinomyces viscosus

full scientific name: Actinomyces viscosus (Howell et al. 1965) Georg et al. 1969

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no94.125
69480100positive
123875nopositiverod-shaped

colony morphology

@refincubation periodhemolysis ability
461101-2 days
1238751

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40539MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
10957PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yesName: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled waterhttps://mediadive.dsmz.de/medium/104
10957COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
123875CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
40539positivegrowth37mesophilic
10957positivegrowth37mesophilic
46110positivegrowth37mesophilic
67770positivegrowth37mesophilic
123875positivegrowth30-37mesophilic
123875nogrowth10psychrophilic
123875nogrowth25mesophilic
123875nogrowth41thermophilic
123875nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
10957anaerobe
10957microaerophile
46110anaerobe
46110microaerophile
69480anaerobe90.905
123875facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.997

halophily

@refsaltgrowthtested relationconcentration
123875NaClpositivegrowth0-2 %
123875NaClnogrowth4 %
123875NaClnogrowth6 %
123875NaClnogrowth8 %
123875NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12387516947citrate-carbon source
1238754853esculin-hydrolysis
123875606565hippurate-hydrolysis
12387517632nitrate+reduction
12387516301nitrite-reduction
12387517632nitrate+respiration
6837917632nitrate+reduction
683794853esculin-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose+fermentation
6837917716lactose+fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate+reduction
6838027897tryptophan-energy source

antibiotic resistance

  • @ref: 123875
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
12387535581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6838035581indole-
12387515688acetoin-
12387517234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68379gelatinase-
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
123875oxidase-
123875beta-galactosidase+3.2.1.23
123875alcohol dehydrogenase-1.1.1.1
123875gelatinase-
123875amylase+
123875DNase-
123875caseinase+3.4.21.50
123875catalase+1.11.1.6
123875tween esterase+/-
123875gamma-glutamyltransferase-2.3.2.2
123875lecithinase-
123875lysine decarboxylase-4.1.1.18
123875ornithine decarboxylase-4.1.1.17
123875phenylalanine ammonia-lyase-4.3.1.24
123875tryptophan deaminase-
123875urease+3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
10957+--+/--+------+---+++-+
46110+--+-+---+--+---+++--

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123875-+++-++---+-++-++---

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
10957--++-++---++--+-+-+-++-++/-----

Isolation, sampling and environmental information

isolation

@refsample type
10957periodontal disease in hamsters
46110Hamster,naturally occurring periodontal disease
67770Periodontal disease in hamsters
123875Animal, Hamster, periodontal disease

isolation source categories

Cat1Cat2
#Infection#Inflammation
#Host Body-Site#Oral cavity and airways

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
10957yes2Risk group (German classification)
1238752Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinomyces viscosus 16S ribosomal RNA gene, partial sequenceAF058051269ena1656
20218Actinomyces viscosus gene for 16S ribosomal RNA, partial sequence, strain: JCM 8353AB5384361502ena1656
20218A.viscosus 16S rRNA geneX824531417ena1656
20218Streptomyces sp. T6 16S ribosomal RNA gene, partial sequenceFJ9419321160ena646657

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinomyces viscosus NCTC10951GCA_900637975completencbi1656
66792Actinomyces viscosus strain CCUG 144761656.9wgspatric1656
66792Actinomyces viscosus strain NCTC109511656.7completepatric1656
66792Actinomyces viscosus NCTC 109512868063378completeimg1656
66792Actinomyces viscosus CCUG 144762868359304draftimg1656
67770Actinomyces viscosus CCUG 14476GCA_004525795contigncbi1656

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes65no
flagellatedno96.595no
gram-positiveyes95.067no
anaerobicno69.233no
aerobicno93.447yes
halophileno89.069no
spore-formingno92.941no
thermophileno96.141no
glucose-utilyes87.865no
motileno91.647no
glucose-fermentyes83.869yes

External links

@ref: 10957

culture collection no.: DSM 43327, ATCC 15987, CCUG 14476, NCTC 10951, WVU 745, NIH T-6, JCM 8353, CCUG 35450, CECT 488, CIP 103147, KCTC 15382, NBIMCC 71

straininfo link

  • @ref: 69839
  • straininfo: 49815

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism32137Degradation of levan by Actinomyces viscosus.Miller CH, Somers PJInfect Immun10.1128/iai.22.1.266-274.19781978Actinomyces/growth & development/*metabolism, Fructans/*metabolism, Fructose/metabolism, Hydrogen-Ion Concentration, Hydrolases/metabolism, Inulin/metabolism, Kinetics, Polysaccharides/*metabolism, Polysaccharides, Bacterial/*metabolismEnzymology
Metabolism344220Cell-associated levan of Actinomyces viscosus.Warner TN, Miller CHInfect Immun10.1128/iai.19.2.711-719.19781978Actinomyces/*analysis/immunology/metabolism, Cell Wall/analysis, Dental Plaque/analysis, Fluorescent Antibody Technique, Glucose/metabolism, Hemagglutination Tests, Myeloma Proteins/immunology, Polysaccharides, Bacterial/*analysis/immunology, Sucrose/metabolism
Enzymology2738835The effect of ofloxacin on experimental periodontitis in hamsters infected with Actinomyces viscosus ATCC 15987.Kametaka S, Miyazaki T, Inoue Y, Hayashi S, Takamori A, Miyake Y, Suginaka HJ Periodontol10.1902/jop.1989.60.5.2851989Actinomyces/*drug effects/isolation & purification, Animals, Bone Resorption/pathology, Cricetinae, Dental Plaque Index, Dietary Carbohydrates/administration & dosage, Male, Mesocricetus, Occult Blood, Ofloxacin/*therapeutic use, Periodontal Index, Periodontitis/*drug therapy/pathology, Saliva/analysis, Starch/administration & dosage, Sucrose/administration & dosagePhylogeny
Phylogeny8360358Characterization of Actinomyces with genomic DNA fingerprints and rRNA gene probes.Bowden G, Johnson J, Schachtele CJ Dent Res10.1177/002203459307200802011993Actinomyces/*classification/*genetics, Actinomyces viscosus/classification/genetics, Animals, *Bacterial Typing Techniques, Blotting, Southern, Cricetinae, DNA Fingerprinting/methods, DNA Probes, DNA, Bacterial/*analysis/isolation & purification, DNA, Ribosomal/analysis, Dental Caries/microbiology, Dental Plaque/microbiology, Deoxyribonuclease BamHI, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Agar Gel, Humans, Mouth/microbiology, RNA, Ribosomal/genetics, RatsEnzymology
8556785The effect of various fluoride compounds on the development of experimental root surface caries in hamsters.de Oliveira Cordeiro JGBull Tokyo Med Dent Univ1995Acidulated Phosphate Fluoride/therapeutic use, Actinomyces viscosus, Alveolar Bone Loss/prevention & control, Animals, Cariostatic Agents/*therapeutic use, Copper/therapeutic use, Cricetinae, Dental Plaque/prevention & control, Diet, Cariogenic, Fluorides/therapeutic use, Fluorides, Topical/*therapeutic use, Male, Mesocricetus, Root Caries/microbiology/*prevention & control, Sodium Fluoride/therapeutic use, Tin Fluorides/therapeutic use, Titanium/therapeutic use
8556786Experimental root surface caries in hamsters the development of the disease after inoculations of two types of cariogenic bacteria.de Oliveira Cordeiro JGBull Tokyo Med Dent Univ1995Actinomyces viscosus/*pathogenicity, Alveolar Bone Loss/etiology, Animals, Cricetinae, Dental Plaque/microbiology, Dental Plaque Index, Diet, Cariogenic, Disease Progression, Male, Mesocricetus, Root Caries/complications/*microbiology/pathology, Streptococcus mutans/pathogenicity, Sucrose
Pathogenicity8871114Serum antibodies to commensal oral and gut bacteria vary with age.Percival RS, Marsh PD, Challacombe SJFEMS Immunol Med Microbiol10.1111/j.1574-695X.1996.tb00356.x1996Actinomyces viscosus/immunology, Adult, Age Factors, Aged, Aged, 80 and over, Antibodies, Bacterial/*blood, Enterococcus faecalis/immunology, Escherichia coli/immunology, Humans, Immunoglobulin A/analysis, Immunoglobulin G/analysis, Immunoglobulin M/analysis, Intestinal Mucosa/*microbiology, Middle Aged, Mouth Mucosa/*microbiology, Streptococcus mutans/immunology
Pathogenicity9151645Age-related changes in salivary antibodies to commensal oral and gut biota.Percival RS, Marsh PD, Challacombe SJOral Microbiol Immunol10.1111/j.1399-302x.1997.tb00367.x1997Actinomyces viscosus/immunology, Adult, Aged, Aged, 80 and over, Aging/*immunology, Antibodies, Bacterial/*analysis/biosynthesis, Antibody Specificity, Cohort Studies, Escherichia coli/immunology, Humans, Immunity, Mucosal/physiology, Immunoglobulin A, Secretory/analysis/biosynthesis, Immunoglobulin G/analysis/biosynthesis, Immunoglobulin M/analysis/biosynthesis, Middle Aged, Parotid Gland/metabolism, Saliva/*immunology, Secretory Rate, Streptococcus mutans/immunologyMetabolism
Pathogenicity16713190Antibacterial activities of inorganic agents on six bacteria associated with oral infections by two susceptibility tests.Fang M, Chen JH, Xu XL, Yang PH, Hildebrand HFInt J Antimicrob Agents10.1016/j.ijantimicag.2006.01.0082006Anti-Bacterial Agents/*pharmacology, Bacteria/*drug effects, Microbial Sensitivity Tests/*methods, Mouth Diseases/*microbiology, Silver/pharmacology, Zinc/pharmacology
Cultivation17896487[Initial study on discrimination of oral microorganisms with the metabonomics technique].Li M, Xiao LY, Li JY, Kong XL, Yu JH, Zhou JL, Xiao XR, Zhu Z, Gong QM, Li WHua Xi Kou Qiang Yi Xue Za Zhi2007Culture Media, Magnetic Resonance Spectroscopy, *Metabolomics, *Streptococcus mutans
Pathogenicity18573934Evaluation of the antimicrobial, antioxidant, and anti-inflammatory activities of hydroxychavicol for its potential use as an oral care agent.Sharma S, Khan IA, Ali I, Ali F, Kumar M, Kumar A, Johri RK, Abdullah ST, Bani S, Pandey A, Suri KA, Gupta BD, Satti NK, Dutt P, Qazi GNAntimicrob Agents Chemother10.1128/AAC.00045-082008Actinomyces viscosus/drug effects, Anti-Infective Agents/chemistry/*pharmacology, Anti-Inflammatory Agents/chemistry/*pharmacology, Antioxidants/chemistry/*pharmacology, Cells, Cultured, Chromatography, High Pressure Liquid, Eugenol/*analogs & derivatives/chemistry/pharmacology, Humans, Lipid Peroxidation/drug effects, Microbial Sensitivity Tests, Mouth/*drug effects, Neutrophils/drug effects/metabolism, Plant Extracts/chemistry/pharmacology, Streptococcus mutans/drug effects, Tumor Necrosis Factor-alpha/metabolismMetabolism
Pathogenicity18818469Antibacterial effects of three experimental quaternary ammonium salt (QAS) monomers on bacteria associated with oral infections.Xiao YH, Chen JH, Fang M, Xing XD, Wang H, Wang YJ, Li FJ Oral Sci10.2334/josnusd.50.3232008Anti-Bacterial Agents/chemistry/*pharmacology, Bacteria, Anaerobic/*drug effects, Colony Count, Microbial, Microbial Sensitivity Tests, Molecular Structure, Mouth Diseases/microbiology, Quaternary Ammonium Compounds/chemistry/*pharmacology, Staphylococcus aureus/drug effects
Pathogenicity19776512Comparative evaluation of antimicrobial activity of three cements: new endodontic cement (NEC), mineral trioxide aggregate (MTA) and Portland.Hasan Zarrabi M, Javidi M, Naderinasab M, Gharechahi MJ Oral Sci10.2334/josnusd.51.4372009Aluminum Compounds/pharmacology, Bacteria, Anaerobic/*drug effects, Calcium Compounds/pharmacology, Candida/*drug effects, Dental Cements/*pharmacology, Drug Combinations, Microbial Sensitivity Tests, Oxides/pharmacology, Periapical Periodontitis/microbiology, Root Canal Filling Materials/*pharmacology, Silicates/pharmacology
Pathogenicity30085427A comparative assessment of the antibacterial activity of root canal sealers on 2 Actinomyces species: An in vitro study.Pawinska M, Luczaj-Cepowicz E, Szczurko G, Kierklo A, Marczuk-Kolada G, Leszczynska KAdv Clin Exp Med10.17219/acem/780222019Actinomyces/classification/*drug effects, Anti-Bacterial Agents/*pharmacology, Colony Count, Microbial, Dental Pulp Cavity, Humans, Root Canal Filling Materials/*pharmacologyPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10957Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43327)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43327
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40539Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14913
46110Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 14476)https://www.ccug.se/strain?id=14476
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69839Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49815.1StrainInfo: A central database for resolving microbial strain identifiers
123875Curators of the CIPCollection of Institut Pasteur (CIP 103147)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103147