Strain identifier
BacDive ID: 1559
Type strain:
Species: Anaerobacillus alkalidiazotrophicus
Strain Designation: MS 6
Strain history: <- D. Y. Sorokin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; MS6
NCBI tax ID(s): 472963 (species)
General
@ref: 16380
BacDive-ID: 1559
DSM-Number: 22531
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped
description: Anaerobacillus alkalidiazotrophicus MS 6 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from soda solonchak soil.
NCBI tax id
- NCBI tax id: 472963
- Matching level: species
strain history
- @ref: 16380
- history: <- D. Y. Sorokin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; MS6
doi: 10.13145/bacdive1559.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Anaerobacillus
- species: Anaerobacillus alkalidiazotrophicus
- full scientific name: Anaerobacillus alkalidiazotrophicus (Sorokin et al. 2008) Zavarzina et al. 2010
synonyms
- @ref: 20215
- synonym: Bacillus alkalidiazotrophicus
@ref: 16380
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Anaerobacillus
species: Anaerobacillus alkalidiazotrophicus
full scientific name: Anaerobacillus alkalidiazotrophicus (Sorokin et al. 2008) Zavarzina et al. 2010
strain designation: MS 6
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
32538 | positive | 03-08 µm | 0.6-1.2 µm | rod-shaped | yes | |
69480 | yes | 96.638 | ||||
69480 | positive | 100 |
pigmentation
- @ref: 32538
- production: yes
Culture and growth conditions
culture medium
- @ref: 16380
- name: BACILLUS ALKALIDIAZOTROPHICUS MEDIUM (DSMZ Medium 1208)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1208
- composition: Name: BACILLUS ALKALIDIAZOTROPHICUS MEDIUM (DSMZ Medium 1208) Composition: Na2CO3 22.0 g/l NaHCO3 8.0 g/l NaCl 6.0 g/l Na2SO4 2.8 g/l Glucose 2.0 g/l K2HPO4 1.0 g/l MgCl2 x 6 H2O 0.2 g/l NH4Cl 0.2 g/l Yeast extract 0.05 g/l Na2-EDTA x 2 H2O 0.0052 g/l FeCl2 x 4 H2O 0.0015 g/l NaOH 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l ZnCl2 7e-05 g/l Thiamine HCl 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Riboflavin 5e-05 g/l (DL)-alpha-Lipoic acid 5e-05 g/l Nicotinic acid 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l H3BO3 6e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
16380 | positive | growth | 30 | mesophilic |
32538 | positive | growth | 15-43 | |
32538 | positive | optimum | 33-35 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
32538 | positive | growth | 7.8-10.6 | alkaliphile |
32538 | positive | optimum | 9.5 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
16380 | anaerobe |
32538 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | yes | 100 |
halophily
- @ref: 32538
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 1-6.96 %
observation
- @ref: 32538
- observation: aggregates in chains
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
32538 | 17057 | cellobiose | + | carbon source |
32538 | 28757 | fructose | + | carbon source |
32538 | 17234 | glucose | + | carbon source |
32538 | 17716 | lactose | + | carbon source |
32538 | 17306 | maltose | + | carbon source |
32538 | 29864 | mannitol | + | carbon source |
32538 | 28053 | melibiose | + | carbon source |
32538 | 16634 | raffinose | + | carbon source |
32538 | 17992 | sucrose | + | carbon source |
32538 | 27082 | trehalose | + | carbon source |
enzymes
- @ref: 32538
- value: catalase
- activity: +
- ec: 1.11.1.6
Isolation, sampling and environmental information
isolation
- @ref: 16380
- sample type: soda solonchak soil
- geographic location: north-eastern part of the Mongolian dry steppe, Choibalsan province
- country: Mongolia
- origin.country: MNG
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Condition | #Alkaline |
Safety information
risk assessment
- @ref: 16380
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16380
- description: Bacillus alkalidiazotrophicus strain MS 6 16S ribosomal RNA gene, partial sequence
- accession: EU143680
- length: 1503
- database: ena
- NCBI tax ID: 472963
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Anaerobacillus alkalidiazotrophicus DSM 22531 | GCA_001866055 | contig | ncbi | 472963 |
66792 | Anaerobacillus alkalidiazotrophicus strain DSM 22531 | 472963.4 | wgs | patric | 472963 |
66792 | Anaerobacillus alkalidiazotrophicus DSM 22531 | 2839700685 | draft | img | 472963 |
66792 | Anaerobacillus alkalidiazotrophicus MS 6 | 2698536991 | draft | img | 472963 |
GC content
@ref | GC-content | method |
---|---|---|
16380 | 37.1 | thermal denaturation, midpoint method (Tm) |
32538 | 37.1 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
gram-positive | yes | 84.32 | yes |
anaerobic | no | 76.478 | no |
halophile | yes | 50.699 | no |
spore-forming | yes | 89.444 | no |
glucose-util | yes | 88.074 | no |
aerobic | no | 86.815 | yes |
flagellated | yes | 79.877 | no |
thermophile | no | 91.63 | yes |
motile | yes | 91.246 | yes |
glucose-ferment | no | 82.798 | no |
External links
@ref: 16380
culture collection no.: DSM 22531, NCCB 100213, UNIQEM U377
straininfo link
- @ref: 71204
- straininfo: 359361
literature
- topic: Phylogeny
- Pubmed-ID: 18842875
- title: Bacillus alkalidiazotrophicus sp. nov., a diazotrophic, low salt-tolerant alkaliphile isolated from Mongolian soda soil.
- authors: Sorokin ID, Kravchenko IK, Tourova TP, Kolganova TV, Boulygina ES, Sorokin DY
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.65655-0
- year: 2008
- mesh: Bacillus/chemistry/*classification/*genetics/isolation & purification, Bacterial Proteins/genetics, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Genes, Bacterial, Genes, rRNA, Molecular Sequence Data, Mongolia, Nitrogen Fixation, Oxidoreductases/genetics, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Bicarbonate, *Soil Microbiology
- topic2: Enzymology
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
16380 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22531) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22531 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
32538 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28756 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
71204 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID359361.1 | StrainInfo: A central database for resolving microbial strain identifiers |