Strain identifier

BacDive ID: 1559

Type strain: Yes

Species: Anaerobacillus alkalidiazotrophicus

Strain Designation: MS 6

Strain history: <- D. Y. Sorokin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; MS6

NCBI tax ID(s): 472963 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 16380

BacDive-ID: 1559

DSM-Number: 22531

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Anaerobacillus alkalidiazotrophicus MS 6 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from soda solonchak soil.

NCBI tax id

  • NCBI tax id: 472963
  • Matching level: species

strain history

  • @ref: 16380
  • history: <- D. Y. Sorokin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; MS6

doi: 10.13145/bacdive1559.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Anaerobacillus
  • species: Anaerobacillus alkalidiazotrophicus
  • full scientific name: Anaerobacillus alkalidiazotrophicus (Sorokin et al. 2008) Zavarzina et al. 2010
  • synonyms

    • @ref: 20215
    • synonym: Bacillus alkalidiazotrophicus

@ref: 16380

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Anaerobacillus

species: Anaerobacillus alkalidiazotrophicus

full scientific name: Anaerobacillus alkalidiazotrophicus (Sorokin et al. 2008) Zavarzina et al. 2010

strain designation: MS 6

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32538positive03-08 µm0.6-1.2 µmrod-shapedyes
69480yes96.638
69480positive100

pigmentation

  • @ref: 32538
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 16380
  • name: BACILLUS ALKALIDIAZOTROPHICUS MEDIUM (DSMZ Medium 1208)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1208
  • composition: Name: BACILLUS ALKALIDIAZOTROPHICUS MEDIUM (DSMZ Medium 1208) Composition: Na2CO3 22.0 g/l NaHCO3 8.0 g/l NaCl 6.0 g/l Na2SO4 2.8 g/l Glucose 2.0 g/l K2HPO4 1.0 g/l MgCl2 x 6 H2O 0.2 g/l NH4Cl 0.2 g/l Yeast extract 0.05 g/l Na2-EDTA x 2 H2O 0.0052 g/l FeCl2 x 4 H2O 0.0015 g/l NaOH 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l ZnCl2 7e-05 g/l Thiamine HCl 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Riboflavin 5e-05 g/l (DL)-alpha-Lipoic acid 5e-05 g/l Nicotinic acid 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l H3BO3 6e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16380positivegrowth30mesophilic
32538positivegrowth15-43
32538positiveoptimum33-35mesophilic

culture pH

@refabilitytypepHPH range
32538positivegrowth7.8-10.6alkaliphile
32538positiveoptimum9.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16380anaerobe
32538facultative anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100

halophily

  • @ref: 32538
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 1-6.96 %

observation

  • @ref: 32538
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3253817057cellobiose+carbon source
3253828757fructose+carbon source
3253817234glucose+carbon source
3253817716lactose+carbon source
3253817306maltose+carbon source
3253829864mannitol+carbon source
3253828053melibiose+carbon source
3253816634raffinose+carbon source
3253817992sucrose+carbon source
3253827082trehalose+carbon source

enzymes

  • @ref: 32538
  • value: catalase
  • activity: +
  • ec: 1.11.1.6

Isolation, sampling and environmental information

isolation

  • @ref: 16380
  • sample type: soda solonchak soil
  • geographic location: north-eastern part of the Mongolian dry steppe, Choibalsan province
  • country: Mongolia
  • origin.country: MNG
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Alkaline

Safety information

risk assessment

  • @ref: 16380
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16380
  • description: Bacillus alkalidiazotrophicus strain MS 6 16S ribosomal RNA gene, partial sequence
  • accession: EU143680
  • length: 1503
  • database: ena
  • NCBI tax ID: 472963

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerobacillus alkalidiazotrophicus DSM 22531GCA_001866055contigncbi472963
66792Anaerobacillus alkalidiazotrophicus strain DSM 22531472963.4wgspatric472963
66792Anaerobacillus alkalidiazotrophicus DSM 225312839700685draftimg472963
66792Anaerobacillus alkalidiazotrophicus MS 62698536991draftimg472963

GC content

@refGC-contentmethod
1638037.1thermal denaturation, midpoint method (Tm)
3253837.1

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes84.32yes
anaerobicno76.478no
halophileyes50.699no
spore-formingyes89.444no
glucose-utilyes88.074no
aerobicno86.815yes
flagellatedyes79.877no
thermophileno91.63yes
motileyes91.246yes
glucose-fermentno82.798no

External links

@ref: 16380

culture collection no.: DSM 22531, NCCB 100213, UNIQEM U377

straininfo link

  • @ref: 71204
  • straininfo: 359361

literature

  • topic: Phylogeny
  • Pubmed-ID: 18842875
  • title: Bacillus alkalidiazotrophicus sp. nov., a diazotrophic, low salt-tolerant alkaliphile isolated from Mongolian soda soil.
  • authors: Sorokin ID, Kravchenko IK, Tourova TP, Kolganova TV, Boulygina ES, Sorokin DY
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65655-0
  • year: 2008
  • mesh: Bacillus/chemistry/*classification/*genetics/isolation & purification, Bacterial Proteins/genetics, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Genes, Bacterial, Genes, rRNA, Molecular Sequence Data, Mongolia, Nitrogen Fixation, Oxidoreductases/genetics, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Bicarbonate, *Soil Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16380Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22531)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22531
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32538Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2875628776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71204Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID359361.1StrainInfo: A central database for resolving microbial strain identifiers