Strain identifier
BacDive ID: 15570
Type strain:
Species: Streptomyces castelarensis
Strain Designation: BJ-608
Strain history: KCC S-0978 <-- DSM 40830 <-- H. D. Tresner BJ-608.
NCBI tax ID(s): 68175 (species)
General
@ref: 9833
BacDive-ID: 15570
DSM-Number: 40830
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production
description: Streptomyces castelarensis BJ-608 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.
NCBI tax id
- NCBI tax id: 68175
- Matching level: species
strain history
@ref | history |
---|---|
9833 | <- H.D. Tresner, BJ-608 |
67770 | KCC S-0978 <-- DSM 40830 <-- H. D. Tresner BJ-608. |
doi: 10.13145/bacdive15570.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces castelarensis
- full scientific name: Streptomyces castelarensis (Cercós 1954) Kumar and Goodfellow 2008
synonyms
@ref synonym 20215 Streptomyces rutgersensis subsp. castelarensis 20215 Streptomyces rutgersensis subsp. castelarense
@ref: 9833
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces castelarensis
full scientific name: Streptomyces castelarensis (Cercós 1954) Kumar and Goodfellow 2008
strain designation: BJ-608
type strain: yes
Morphology
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
18677 | Sand yellow (1002) | 10-14 days | ISP 2 |
18677 | Beige (1001) | 10-14 days | ISP 3 |
18677 | Beige (1001) | 10-14 days | ISP 4 |
18677 | Beige (1001) | 10-14 days | ISP 5 |
18677 | Beige (1001) | 10-14 days | ISP 6 |
18677 | Cream (9001) | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
18677 | yes | Aerial Mycelium | Tele grey | ISP 2 |
18677 | yes | Aerial Mycelium | Tele grey | ISP 3 |
18677 | yes | Aerial Mycelium | Tele grey | ISP 4 |
18677 | yes | Aerial Mycelium | Cream | ISP 5 |
18677 | no | ISP 6 | ||
18677 | yes | Aerial Mycelium | Red brown (8012) | ISP 7 |
pigmentation
- @ref: 32383
- production: no
multimedia
- @ref: 9833
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40830.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
9833 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
18677 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
18677 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
18677 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
18677 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
18677 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
18677 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
9833 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf | |
9833 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
18677 | positive | optimum | 28 | mesophilic |
9833 | positive | growth | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
- @ref: 32383
- spore formation: yes
compound production
@ref | compound |
---|---|
9833 | camphomycin |
18677 | Benzonaphthopyran and its rhamnopyranosides |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
18677 | 17234 | glucose | + | |
18677 | 22599 | arabinose | + | |
18677 | 17992 | sucrose | - | |
18677 | 18222 | xylose | - | |
18677 | 17268 | myo-inositol | - | |
18677 | 29864 | mannitol | + | |
18677 | 28757 | fructose | + | |
18677 | 26546 | rhamnose | + | |
18677 | 16634 | raffinose | + | |
18677 | 62968 | cellulose | + | |
32383 | 17057 | cellobiose | + | carbon source |
32383 | 28757 | fructose | + | carbon source |
32383 | 28260 | galactose | + | carbon source |
32383 | 17234 | glucose | + | carbon source |
32383 | 17754 | glycerol | + | carbon source |
32383 | 28087 | glycogen | + | carbon source |
32383 | 17306 | maltose | + | carbon source |
32383 | 29864 | mannitol | + | carbon source |
32383 | 37684 | mannose | + | carbon source |
32383 | 28053 | melibiose | + | carbon source |
32383 | 506227 | N-acetylglucosamine | + | carbon source |
32383 | 16634 | raffinose | + | carbon source |
32383 | 26546 | rhamnose | + | carbon source |
32383 | 33942 | ribose | + | carbon source |
32383 | 27082 | trehalose | + | carbon source |
32383 | 17151 | xylitol | + | carbon source |
32383 | 18222 | xylose | + | carbon source |
metabolite production
- @ref: 67770
- metabolite: camphomycin
- production: yes
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | + | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | + | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
18677 | + | + | + | - | + | + | + | + | + | + | + | - | + | - | + | + | + | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
9833 | dust | Castelar | Argentina | ARG | Middle and South America |
67770 | Dust in Castelar | Argentina | ARG | Middle and South America |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Waste | #Dust (Ash) |
#Environmental | #Terrestrial | #Dust |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
9833 | 1 | Risk group (German classification) |
18677 | 1 | German classification |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptomyces castelarensis strain DSM 40830 16S ribosomal RNA gene, partial sequence | HQ244465 | 1371 | ena | 260811 |
9833 | Streptomyces castelarensis strain DSM 40830 16S ribosomal RNA gene, partial sequence | AY508511 | 1475 | ena | 260811 |
9833 | Streptomyces antimycoticus gene for 16S rRNA, partial sequence, strain: NBRC 15875 | AB184709 | 1436 | ena | 68175 |
Genome sequences
- @ref: 67770
- description: Streptomyces antimycoticus NRRL B-24289
- accession: GCA_002154275
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 68175
GC content
- @ref: 67770
- GC-content: 70.7
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 98.29 | no |
gram-positive | yes | 84.53 | no |
anaerobic | no | 98.926 | no |
halophile | no | 90.846 | no |
spore-forming | yes | 93.915 | yes |
glucose-util | yes | 90.05 | yes |
aerobic | yes | 91.772 | no |
thermophile | no | 97.915 | yes |
motile | no | 93.123 | no |
glucose-ferment | no | 88.803 | no |
External links
@ref: 9833
culture collection no.: DSM 40830, ATCC 15191, CBS 309.55, IMRU 3559, JCM 4978, BCRC 11879, IFO 15875, INA R-43, NBRC 15875, NRRL B-24289, RIA 851, VKM Ac-832
straininfo link
- @ref: 84657
- straininfo: 36301
literature
- topic: Phylogeny
- Pubmed-ID: 18523180
- title: Five new members of the Streptomyces violaceusniger 16S rRNA gene clade: Streptomyces castelarensis sp. nov., comb. nov., Streptomyces himastatinicus sp. nov., Streptomyces mordarskii sp. nov., Streptomyces rapamycinicus sp. nov. and Streptomyces ruanii sp. nov.
- authors: Kumar Y, Goodfellow M
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.65408-0
- year: 2008
- mesh: Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, *Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, Species Specificity, Streptomyces/*classification/genetics/physiology
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
---|---|---|---|---|---|---|---|
9833 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40830) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40830 | ||||
18677 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40830.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
32383 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 28609 | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
68382 | Automatically annotated from API zym | ||||||
84657 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID36301.1 | StrainInfo: A central database for resolving microbial strain identifiers |