Strain identifier

BacDive ID: 15570

Type strain: Yes

Species: Streptomyces castelarensis

Strain Designation: BJ-608

Strain history: KCC S-0978 <-- DSM 40830 <-- H. D. Tresner BJ-608.

NCBI tax ID(s): 68175 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9833

BacDive-ID: 15570

DSM-Number: 40830

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces castelarensis BJ-608 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 68175
  • Matching level: species

strain history

@refhistory
9833<- H.D. Tresner, BJ-608
67770KCC S-0978 <-- DSM 40830 <-- H. D. Tresner BJ-608.

doi: 10.13145/bacdive15570.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces castelarensis
  • full scientific name: Streptomyces castelarensis (Cercós 1954) Kumar and Goodfellow 2008
  • synonyms

    @refsynonym
    20215Streptomyces rutgersensis subsp. castelarensis
    20215Streptomyces rutgersensis subsp. castelarense

@ref: 9833

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces castelarensis

full scientific name: Streptomyces castelarensis (Cercós 1954) Kumar and Goodfellow 2008

strain designation: BJ-608

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18677Sand yellow (1002)10-14 daysISP 2
18677Beige (1001)10-14 daysISP 3
18677Beige (1001)10-14 daysISP 4
18677Beige (1001)10-14 daysISP 5
18677Beige (1001)10-14 daysISP 6
18677Cream (9001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18677yesAerial MyceliumTele greyISP 2
18677yesAerial MyceliumTele greyISP 3
18677yesAerial MyceliumTele greyISP 4
18677yesAerial MyceliumCreamISP 5
18677noISP 6
18677yesAerial MyceliumRed brown (8012)ISP 7

pigmentation

  • @ref: 32383
  • production: no

multimedia

  • @ref: 9833
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40830.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9833GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18677ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18677ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18677ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18677ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18677ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18677ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9833STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf
9833ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
18677positiveoptimum28mesophilic
9833positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 32383
  • spore formation: yes

compound production

@refcompound
9833camphomycin
18677Benzonaphthopyran and its rhamnopyranosides

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1867717234glucose+
1867722599arabinose+
1867717992sucrose-
1867718222xylose-
1867717268myo-inositol-
1867729864mannitol+
1867728757fructose+
1867726546rhamnose+
1867716634raffinose+
1867762968cellulose+
3238317057cellobiose+carbon source
3238328757fructose+carbon source
3238328260galactose+carbon source
3238317234glucose+carbon source
3238317754glycerol+carbon source
3238328087glycogen+carbon source
3238317306maltose+carbon source
3238329864mannitol+carbon source
3238337684mannose+carbon source
3238328053melibiose+carbon source
32383506227N-acetylglucosamine+carbon source
3238316634raffinose+carbon source
3238326546rhamnose+carbon source
3238333942ribose+carbon source
3238327082trehalose+carbon source
3238317151xylitol+carbon source
3238318222xylose+carbon source

metabolite production

  • @ref: 67770
  • metabolite: camphomycin
  • production: yes

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18677+++-+++++++-+-++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9833dustCastelarArgentinaARGMiddle and South America
67770Dust in CastelarArgentinaARGMiddle and South America

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Dust (Ash)
#Environmental#Terrestrial#Dust

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
98331Risk group (German classification)
186771German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces castelarensis strain DSM 40830 16S ribosomal RNA gene, partial sequenceHQ2444651371ena260811
9833Streptomyces castelarensis strain DSM 40830 16S ribosomal RNA gene, partial sequenceAY5085111475ena260811
9833Streptomyces antimycoticus gene for 16S rRNA, partial sequence, strain: NBRC 15875AB1847091436ena68175

Genome sequences

  • @ref: 67770
  • description: Streptomyces antimycoticus NRRL B-24289
  • accession: GCA_002154275
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 68175

GC content

  • @ref: 67770
  • GC-content: 70.7
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.29no
gram-positiveyes84.53no
anaerobicno98.926no
halophileno90.846no
spore-formingyes93.915yes
glucose-utilyes90.05yes
aerobicyes91.772no
thermophileno97.915yes
motileno93.123no
glucose-fermentno88.803no

External links

@ref: 9833

culture collection no.: DSM 40830, ATCC 15191, CBS 309.55, IMRU 3559, JCM 4978, BCRC 11879, IFO 15875, INA R-43, NBRC 15875, NRRL B-24289, RIA 851, VKM Ac-832

straininfo link

  • @ref: 84657
  • straininfo: 36301

literature

  • topic: Phylogeny
  • Pubmed-ID: 18523180
  • title: Five new members of the Streptomyces violaceusniger 16S rRNA gene clade: Streptomyces castelarensis sp. nov., comb. nov., Streptomyces himastatinicus sp. nov., Streptomyces mordarskii sp. nov., Streptomyces rapamycinicus sp. nov. and Streptomyces ruanii sp. nov.
  • authors: Kumar Y, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65408-0
  • year: 2008
  • mesh: Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, *Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, Species Specificity, Streptomyces/*classification/genetics/physiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
9833Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40830)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40830
18677Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40830.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32383Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604128609
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
84657Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36301.1StrainInfo: A central database for resolving microbial strain identifiers