Strain identifier

BacDive ID: 15499

Type strain: Yes

Species: Streptomyces purpurascens

Strain Designation: Maria 515

Strain history: KCC S-0509 <-- IFO 13077 <-- SAJ <-- ISP 5310 <-- P. Wilde Maria 515.

NCBI tax ID(s): 1924 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 9450

BacDive-ID: 15499

DSM-Number: 40310

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces purpurascens Maria 515 is a spore-forming, mesophilic bacterium that produces antibiotic compounds and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1924
  • Matching level: species

strain history

@refhistory
9450<- E.B. Shirling, ISP <- P. Wilde, Maria 515
67770KCC S-0509 <-- IFO 13077 <-- SAJ <-- ISP 5310 <-- P. Wilde Maria 515.

doi: 10.13145/bacdive15499.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces purpurascens
  • full scientific name: Streptomyces purpurascens Lindenbein 1952 (Approved Lists 1980)

@ref: 9450

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces purpurascens

full scientific name: Streptomyces purpurascens Lindenbein 1952

strain designation: Maria 515

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no90.227
69480100positive

multimedia

  • @ref: 9450
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40310.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9450GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
9450ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
19436positiveoptimum30mesophilic
9450positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

tolerance

  • @ref: 19436
  • compound: Lysozyme
  • percentage: 0.1

spore formation

@refspore formationconfidence
69481yes100
69480yes100

compound production

@refcompound
9450isorhodomycin A
9450rhodomycin A
9450rhodomycin B
67770Rhodomycins

halophily

  • @ref: 19436
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 7.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1943616634raffinose+
1943626546rhamnose+
1943628757fructose+
1943629864mannitol+
1943617268myo-inositol+
1943618222xylose-
1943617992sucrose+
1943622599arabinose+/-
1943617234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan+energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole+

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19436------+-+++

Isolation, sampling and environmental information

isolation

  • @ref: 9450
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
94501Risk group (German classification)
194361Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces purpurascens partial 16S rRNA gene, type strain DSM 40310AJ3109251448ena1924
20218Streptomyces purpurascens partial 16S rRNA gene, strain ISP 5310AJ3994861448ena1924
20218Streptomyces purpurascens gene for 16S rRNAAB0458881484ena1924
20218Streptomyces purpurascens gene for 16S ribosomal RNA, partial sequence, strain: JCM 4509D44199121ena1924
20218Streptomyces purpurascens gene for 16S rRNA, partial sequence, strain: NBRC 13077AB1848591473ena1924
67770Streptomyces purpurascens 16S rRNA gene, type strain LMG 20526AJ7813821479ena1924

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces purpurascens JCM 4509GCA_014650155scaffoldncbi1924
66792Streptomyces purpurascens strain JCM 45091924.5wgspatric1924
66792Streptomyces purpurascens DSM 40310GCA_021390235contigncbi1924

GC content

  • @ref: 67770
  • GC-content: 72
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno97.81no
gram-positiveyes88.25no
anaerobicno98.912no
aerobicyes92.986no
halophileno93.94no
spore-formingyes95.306no
glucose-utilyes89.777yes
thermophileno98.39yes
motileno93.806no
glucose-fermentno90.411no

External links

@ref: 9450

culture collection no.: DSM 40310, ATCC 25489, CBS 917.69, IFO 13077, ISP 5310, JCM 4509, NBRC 13077, RIA 1269, BCRC 11872, KCTC 9848, NBIMCC 1155, NRRL B-12230, PCM 2299, VKM Ac-755

straininfo link

  • @ref: 84585
  • straininfo: 389278

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism8081500Hybrid anthracycline antibiotics: production of new anthracyclines by cloned genes from Streptomyces purpurascens in Streptomyces galilaeus.Niemi J, Ylihonko K, Hakala J, Parssinen R, Kopio A, Mantsala PMicrobiology (Reading)10.1099/00221287-140-6-13511994Acyltransferases/*genetics/metabolism, Aminoglycosides/*biosynthesis/chemistry/pharmacology, Animals, *Anthracyclines, Anthraquinones/metabolism, Antibiotics, Antineoplastic/*biosynthesis/chemistry/pharmacology, Bacterial Proteins/*genetics/metabolism, Cloning, Molecular, DNA, Bacterial/*genetics, Gene Expression Regulation, Bacterial, *Genes, Bacterial, Leukemia L1210, Mice, Molecular Structure, Naphthacenes/metabolism, Polyketide Synthases, Recombination, Genetic, Sequence Deletion, Species Specificity, Streptomyces/classification/*genetics, Tumor Cells, Cultured/drug effectsPhylogeny
Phylogeny28141499Streptomyces jeddahensis sp. nov., an oleaginous bacterium isolated from desert soil.Rottig A, Atasayar E, Meier-Kolthoff JP, Sproer C, Schumann P, Schauer J, Steinbuchel AInt J Syst Evol Microbiol10.1099/ijsem.0.0018392017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saudi Arabia, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny33415367Streptomyces cupreus sp. nov., an antimicrobial producing actinobacterium isolated from Himalayan soil.Maiti PK, Mandal SArch Microbiol10.1007/s00203-020-02160-y2021DNA, Bacterial/genetics, India, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Species Specificity, *Streptomyces/classification/genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9450Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40310)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40310
19436Wink, J.Streptomyces purpurascensCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84585Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389278.1StrainInfo: A central database for resolving microbial strain identifiers