Archive BacDiveID:10.13145/bacdive15488.20230509.8.1

Strain identifier

BacDive ID: 15488

Type strain:

Species: Streptomyces pseudoechinosporeus

Strain Designation: LIA-0442

Strain history: KCC A-0066 <-- V. A. Tsyganov P-147 (LIA 0442).

NCBI tax ID(s): 285444 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 10705

BacDive-ID: 15488

DSM-Number: 43035

keywords: 16S sequence, Bacteria, mesophilic

description: Streptomyces pseudoechinosporeus LIA-0442 is a mesophilic bacterium that builds an aerial mycelium and was isolated from sandy desert soil.

NCBI tax id

NCBI tax id: 285444
Matching level: species

strain history

@ref	history
10705	<- KCC (Microechinospora grisea) <- V.A. Tsyganov, LIA, 0442
67770	KCC A-0066 <-- V. A. Tsyganov P-147 (LIA 0442).

doi: 10.13145/bacdive15488.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/actinomycetota
domain: Bacteria
phylum: Actinomycetota
class: Actinomycetes
order: Kitasatosporales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces pseudoechinosporeus
full scientific name: Streptomyces pseudoechinosporeus Goodfellow et al. 1986
synonyms
@ref synonym
20215 Microechinospora grisea
20215 Microellobosporia grisea

@ref	synonym
20215	Microechinospora grisea
20215	Microellobosporia grisea

@ref: 10705

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces pseudoechinosporeus

full scientific name: Streptomyces pseudoechinosporeus Goodfellow et al. 1986

strain designation: LIA-0442

type strain: yes

Morphology

colony morphology

@ref	colony color	incubation period	medium used
19929	Colourless	10-14 days	ISP 2
19929	Colourless	10-14 days	ISP 3
19929	Colourless	10-14 days	ISP 4
19929	Colourless	10-14 days	ISP 5
19929	Colourless	10-14 days	ISP 6
19929	Beige (1001)	10-14 days	ISP 7

multicellular morphology

@ref	forms multicellular complex	medium name	complex name	complex color
19929	no	ISP 2
19929	no	ISP 3
19929	no	ISP 4
19929	no	ISP 5
19929	no	ISP 6
19929	yes	ISP 7	Aerial Mycelium	White

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
10705	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	yes	https://mediadive.dsmz.de/medium/65	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19929	ISP 2	yes		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19929	ISP 3	yes		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19929	ISP 4	yes		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19929	ISP 5	yes		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19929	ISP 6	yes		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19929	ISP 7	yes		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10705	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	yes	https://mediadive.dsmz.de/medium/84	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
19929	positive	optimum	28	mesophilic
10705	positive	growth	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

compound production

@ref: 20216
compound: Antifungal activity

observation

@ref: 67770
observation: quinones: MK-9(H₆), MK-9(H₈), MK-9(H₄)

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
19929	62968	cellulose	-
19929	16634	raffinose	+/-
19929	26546	rhamnose	+
19929	28757	fructose	+
19929	29864	mannitol	+
19929	17268	myo-inositol	+
19929	18222	xylose	-
19929	17992	sucrose	+
19929	22599	arabinose	+
19929	17234	glucose	+
68368	5291	gelatin	+	hydrolysis
68368	27897	tryptophan	-	energy source
68368	16199	urea	-	hydrolysis
68368	16947	citrate	-	assimilation
68368	18257	ornithine	-	degradation
68368	25094	lysine	-	degradation
68368	29016	arginine	-	hydrolysis

metabolite production

@ref	Chebi-ID	metabolite	production
68368	15688	acetoin	yes
68368	35581	indole	no
68368	16136	hydrogen sulfide	no

metabolite tests

@ref	Chebi-ID	metabolite	voges-proskauer-test	indole test
68368	15688	acetoin	+
68368	35581	indole		-

enzymes

@ref	value	activity	ec
68368	gelatinase	+
68368	tryptophan deaminase	-	4.1.99.1
68368	urease	-	3.5.1.5
68368	ornithine decarboxylase	-	4.1.1.17
68368	lysine decarboxylase	-	4.1.1.18
68368	arginine dihydrolase	-	3.5.3.6
68368	beta-galactosidase	+	3.2.1.23

API 20E

@ref	ONPG	ADH Arg	LDC Lys	ODC	CIT	H2S	URE	TDA Trp	IND	VP	GEL
19929	+	-	-	-	-	-	-	-	-	+	+

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country
10705	sandy desert soil
67770	Soil	Kyzyl-Kum desert	USSR

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Desert
#Environmental	#Terrestrial	#Sandy
#Environmental	#Terrestrial	#Soil

Safety information

risk assessment

@ref	biosafety level	biosafety level comment
10705	1	Risk group (German classification)
19929	1	German classification

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Streptomyces pseudoechinosporeus gene for 16S rRNA, partial sequence, strain: NBRC 12518	AB184100	1448	ena	285444
67770	Streptomyces pseudoechinosporeus JCM 3066 gene for 16S rRNA, partial sequence	LC506593	993	ena	285444

GC content

@ref: 67770
GC-content: 69.1

External links

@ref: 10705

culture collection no.: DSM 43035, ATCC 19618, CUB 161, IFO 12518, KCC A-0066, NBRC 12518, LIA (P 147), JCM 3066, CGMCC 4.1207, CGMCC 4.1228, IFM 1243, IMET 43494, KCTC 9178, NBIMCC 496, NCIMB 9918, NRRL B-16931, RIA 554, RIA 897, VKM Ac-1226

straininfo link

@ref: 84575
straininfo: 265366

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
10705	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43035)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-43035
19929	Wink, J.		http://www.dsmz.de/microorganisms/wink_pdf/DSM43035.pdf	Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216	Curators of the HKI		http://www.leibniz-hki.de/de/	Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
68368				Automatically annotated from API 20E
84575	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID265366.1	StrainInfo: A central database for resolving microbial strain identifiers

General

NCBI tax id

strain history

Name and taxonomic classification

LPSN

synonyms

Morphology

colony morphology

multicellular morphology

Culture and growth conditions

culture medium

culture temp

Physiology and metabolism

compound production

observation

metabolite utilization

metabolite production

metabolite tests

enzymes

API 20E

Isolation, sampling and environmental information

isolation

isolation source categories

Safety information

risk assessment

Sequence information

16S sequences

GC content

External links

straininfo link

Reference