Strain identifier
BacDive ID: 15487
Type strain:
Species: Kitasatospora aureofaciens
Strain history: KCC S-0434 <-- CUB 514 <-- Boots FD 1323 <-- ATCC 14125 <-- Lepetit Labs.; C1 7190.
NCBI tax ID(s): 1894 (species)
General
@ref: 9472
BacDive-ID: 15487
DSM-Number: 40341
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, antibiotic compound production
description: Kitasatospora aureofaciens DSM 40341 is a mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.
NCBI tax id
- NCBI tax id: 1894
- Matching level: species
strain history
@ref | history |
---|---|
9472 | <- E.B. Shirling, ISP <- CBS (S 4623/33 = P 19) |
67770 | KCC S-0434 <-- CUB 514 <-- Boots FD 1323 <-- ATCC 14125 <-- Lepetit Labs.; C1 7190. |
doi: 10.13145/bacdive15487.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Kitasatospora
- species: Kitasatospora aureofaciens
- full scientific name: Kitasatospora aureofaciens (Duggar 1948) Labeda et al. 2017
synonyms
@ref synonym 20215 Streptomyces avellaneus 20215 Streptomyces aureofaciens 20215 Streptomyces psammoticus 20215 Kitasatospora psammotica
@ref: 9472
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Kitasatospora
species: Kitasatospora psammotica
full scientific name: Kitasatospora psammotica (Virgilio and Hengeller 1960) Labeda et al. 2017
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 93.393 | |
69480 | 100 | positive |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19441 | Yellow | 10-14 days | ISP 2 |
19441 | Beige green | 10-14 days | ISP 3 |
19441 | Beige green | 10-14 days | ISP 4 |
19441 | Yellow green | 10-14 days | ISP 5 |
19441 | Brown | 10-14 days | ISP 6 |
19441 | Yellow | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
19441 | yes | Aerial Mycelium | Yellow green | ISP 2 |
19441 | yes | Aerial Mycelium | Green | ISP 3 |
19441 | yes | Aerial Mycelium | Beige green | ISP 4 |
19441 | yes | Aerial Mycelium | Yellow | ISP 5 |
19441 | no | ISP 6 | ||
19441 | yes | Aerial Mycelium | Yellow | ISP 7 |
multimedia
- @ref: 9472
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40341.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | composition |
---|---|---|---|
19441 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes |
19441 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: |
19441 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml |
19441 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes |
19441 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes |
19441 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19441 | positive | optimum | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
tolerance
- @ref: 19441
- compound: Lysozyme
- percentage: 0.1
spore formation
@ref | spore description | spore formation | confidence |
---|---|---|---|
19441 | Formation of spore chains: rectiflixibilis, spore surface: smooth | no | |
69481 | yes | 100 | |
69480 | yes | 100 |
compound production
- @ref: 9472
- compound: 6 demethyltetracyclines
halophily
- @ref: 19441
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 2.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
19441 | 62968 | cellulose | - | |
19441 | 16634 | raffinose | - | |
19441 | 26546 | rhamnose | + | |
19441 | 28757 | fructose | - | |
19441 | 29864 | mannitol | - | |
19441 | 17268 | myo-inositol | - | |
19441 | 18222 | xylose | +/- | |
19441 | 17992 | sucrose | - | |
19441 | 22599 | arabinose | + | |
19441 | 17234 | glucose | + | |
68368 | 5291 | gelatin | - | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | - | hydrolysis |
68368 | 16947 | citrate | + | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | - | hydrolysis |
metabolite production
@ref | metabolite | production | Chebi-ID |
---|---|---|---|
67770 | demecycline | yes | |
68368 | acetoin | yes | 15688 |
68368 | indole | no | 35581 |
68368 | hydrogen sulfide | no | 16136 |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | + | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | gelatinase | - | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
19441 | + | - | - | - | + | - | - | - | - | + | - |
Isolation, sampling and environmental information
taxonmaps
- @ref: 69479
- File name: preview.99_224.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_148;97_159;98_191;99_224&stattab=map
- Last taxonomy: Streptomycetaceae
- 16S sequence: AB184554
- Sequence Identity:
- Total samples: 4218
- soil counts: 3325
- aquatic counts: 173
- animal counts: 599
- plant counts: 121
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
9472 | 1 | Risk group (German classification) |
19441 | 1 | Risk group (German classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptomyces psammoticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4434 | D44141 | 120 | ena | 67349 |
20218 | Streptomyces psammoticus gene for 16S rRNA, partial sequence, strain: NBRC 13971 | AB184554 | 1472 | ena | 67349 |
9472 | Streptomyces psammoticus strain IFO 13971 16S ribosomal RNA gene, partial sequence | AY999862 | 1420 | ena | 67349 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Kitasatospora aureofaciens JCM 4434 | GCA_014649975 | contig | ncbi | 1894 |
66792 | Kitasatospora psammotica strain JCM 4434 | 67349.3 | wgs | patric | 67349 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
gram-positive | yes | 93.422 | no |
anaerobic | no | 99.055 | no |
halophile | no | 95.645 | no |
spore-forming | yes | 91.514 | yes |
glucose-util | yes | 90.668 | yes |
thermophile | no | 96.787 | no |
flagellated | no | 98.139 | no |
aerobic | yes | 90.422 | no |
motile | no | 94.374 | no |
glucose-ferment | no | 90.582 | no |
External links
@ref: 9472
culture collection no.: DSM 40341, ATCC 14125, ATCC 25488, CBS 175.61, IFO 13971, JCM 4434, NBRC 13971, RIA 1268, RIA 832, ISP 5341, KCC S-0434, BCRC 12241, CBS 299.65, CBS 916.69, CGMCC 4.1465, IFO 13076, IFO 13877, KCTC 19966, NBRC 13076, NBRC 13877, NRRL B-3291, NRRL B-5753, NRRL ISP-5341, PCM 2371, VKM Ac-996
straininfo link
- @ref: 84574
- straininfo: 92813
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 23820614 | Langkocyclines: novel angucycline antibiotics from Streptomyces sp. Acta 3034(*). | Kalyon B, Tan GY, Pinto JM, Foo CY, Wiese J, Imhoff JF, Sussmuth RD, Sabaratnam V, Fiedler HP | J Antibiot (Tokyo) | 10.1038/ja.2013.53 | 2013 | Animals, *Anthraquinones/chemistry/metabolism/pharmacology, *Anti-Bacterial Agents/chemistry/metabolism/pharmacology, Antineoplastic Agents/*pharmacology, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis, Gram-Positive Bacteria/*drug effects, Hep G2 Cells, Humans, Malaysia, Mice, Models, Molecular, Molecular Sequence Data, NIH 3T3 Cells, *Oligosaccharides/chemistry/metabolism/pharmacology, Phylogeny, *Polycyclic Aromatic Hydrocarbons/chemistry/metabolism/pharmacology, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification/*metabolism, Structure-Activity Relationship | Metabolism |
Phylogeny | 28884662 | Streptomyces cerasinus sp. nov., isolated from soil in Thailand. | Kanchanasin P, Moonmangmee D, Phongsopitanun W, Tanasupawat S, Moonmangmee S | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002212 | 2017 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Thailand, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
9472 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40341) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40341 | |||
19441 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40341.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
84574 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92813.1 | StrainInfo: A central database for resolving microbial strain identifiers |