Strain identifier

BacDive ID: 15487

Type strain: Yes

Species: Kitasatospora aureofaciens

Strain history: KCC S-0434 <-- CUB 514 <-- Boots FD 1323 <-- ATCC 14125 <-- Lepetit Labs.; C1 7190.

NCBI tax ID(s): 1894 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9472

BacDive-ID: 15487

DSM-Number: 40341

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, antibiotic compound production

description: Kitasatospora aureofaciens DSM 40341 is a mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 1894
  • Matching level: species

strain history

@refhistory
9472<- E.B. Shirling, ISP <- CBS (S 4623/33 = P 19)
67770KCC S-0434 <-- CUB 514 <-- Boots FD 1323 <-- ATCC 14125 <-- Lepetit Labs.; C1 7190.

doi: 10.13145/bacdive15487.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Kitasatospora
  • species: Kitasatospora aureofaciens
  • full scientific name: Kitasatospora aureofaciens (Duggar 1948) Labeda et al. 2017
  • synonyms

    @refsynonym
    20215Streptomyces avellaneus
    20215Streptomyces aureofaciens
    20215Streptomyces psammoticus
    20215Kitasatospora psammotica

@ref: 9472

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Kitasatospora

species: Kitasatospora psammotica

full scientific name: Kitasatospora psammotica (Virgilio and Hengeller 1960) Labeda et al. 2017

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no93.393
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
19441Yellow10-14 daysISP 2
19441Beige green10-14 daysISP 3
19441Beige green10-14 daysISP 4
19441Yellow green10-14 daysISP 5
19441Brown10-14 daysISP 6
19441Yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19441yesAerial MyceliumYellow greenISP 2
19441yesAerial MyceliumGreenISP 3
19441yesAerial MyceliumBeige greenISP 4
19441yesAerial MyceliumYellowISP 5
19441noISP 6
19441yesAerial MyceliumYellowISP 7

multimedia

  • @ref: 9472
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40341.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthcomposition
19441ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19441ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19441ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19441ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19441ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19441ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19441positiveoptimum28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

tolerance

  • @ref: 19441
  • compound: Lysozyme
  • percentage: 0.1

spore formation

@refspore descriptionspore formationconfidence
19441Formation of spore chains: rectiflixibilis, spore surface: smoothno
69481yes100
69480yes100

compound production

  • @ref: 9472
  • compound: 6 demethyltetracyclines

halophily

  • @ref: 19441
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1944162968cellulose-
1944116634raffinose-
1944126546rhamnose+
1944128757fructose-
1944129864mannitol-
1944117268myo-inositol-
1944118222xylose+/-
1944117992sucrose-
1944122599arabinose+
1944117234glucose+
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refmetaboliteproductionChebi-ID
67770demecyclineyes
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19441+---+----+-

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_224.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_148;97_159;98_191;99_224&stattab=map
  • Last taxonomy: Streptomycetaceae
  • 16S sequence: AB184554
  • Sequence Identity:
  • Total samples: 4218
  • soil counts: 3325
  • aquatic counts: 173
  • animal counts: 599
  • plant counts: 121

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
94721Risk group (German classification)
194411Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces psammoticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4434D44141120ena67349
20218Streptomyces psammoticus gene for 16S rRNA, partial sequence, strain: NBRC 13971AB1845541472ena67349
9472Streptomyces psammoticus strain IFO 13971 16S ribosomal RNA gene, partial sequenceAY9998621420ena67349

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kitasatospora aureofaciens JCM 4434GCA_014649975contigncbi1894
66792Kitasatospora psammotica strain JCM 443467349.3wgspatric67349

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes93.422no
anaerobicno99.055no
halophileno95.645no
spore-formingyes91.514yes
glucose-utilyes90.668yes
thermophileno96.787no
flagellatedno98.139no
aerobicyes90.422no
motileno94.374no
glucose-fermentno90.582no

External links

@ref: 9472

culture collection no.: DSM 40341, ATCC 14125, ATCC 25488, CBS 175.61, IFO 13971, JCM 4434, NBRC 13971, RIA 1268, RIA 832, ISP 5341, KCC S-0434, BCRC 12241, CBS 299.65, CBS 916.69, CGMCC 4.1465, IFO 13076, IFO 13877, KCTC 19966, NBRC 13076, NBRC 13877, NRRL B-3291, NRRL B-5753, NRRL ISP-5341, PCM 2371, VKM Ac-996

straininfo link

  • @ref: 84574
  • straininfo: 92813

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23820614Langkocyclines: novel angucycline antibiotics from Streptomyces sp. Acta 3034(*).Kalyon B, Tan GY, Pinto JM, Foo CY, Wiese J, Imhoff JF, Sussmuth RD, Sabaratnam V, Fiedler HPJ Antibiot (Tokyo)10.1038/ja.2013.532013Animals, *Anthraquinones/chemistry/metabolism/pharmacology, *Anti-Bacterial Agents/chemistry/metabolism/pharmacology, Antineoplastic Agents/*pharmacology, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis, Gram-Positive Bacteria/*drug effects, Hep G2 Cells, Humans, Malaysia, Mice, Models, Molecular, Molecular Sequence Data, NIH 3T3 Cells, *Oligosaccharides/chemistry/metabolism/pharmacology, Phylogeny, *Polycyclic Aromatic Hydrocarbons/chemistry/metabolism/pharmacology, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification/*metabolism, Structure-Activity RelationshipMetabolism
Phylogeny28884662Streptomyces cerasinus sp. nov., isolated from soil in Thailand.Kanchanasin P, Moonmangmee D, Phongsopitanun W, Tanasupawat S, Moonmangmee SInt J Syst Evol Microbiol10.1099/ijsem.0.0022122017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Thailand, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9472Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40341)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40341
19441Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40341.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84574Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92813.1StrainInfo: A central database for resolving microbial strain identifiers