Strain identifier

BacDive ID: 15463

Type strain: Yes

Species: Streptomyces phaeochromogenes

Strain history: KCC S-0070 <-- H. Yonehara <-- IFO 3180 <-- ATCC 3338 <-- IMRU 3338.

NCBI tax ID(s): 1923 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9271

BacDive-ID: 15463

DSM-Number: 40073

keywords: spore-forming, Bacteria, 16S sequence, genome sequence, mesophilic

description: Streptomyces phaeochromogenes DSM 40073 is a spore-forming, mesophilic bacterium that builds an aerial mycelium.

NCBI tax id

  • NCBI tax id: 1923
  • Matching level: species

strain history

doi: 10.13145/bacdive15463.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces phaeochromogenes
  • full scientific name: Streptomyces phaeochromogenes (Conn 1917) Waksman 1957 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Actinomyces pheochromogenus

@ref: 9271

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces phaeochromogenes

full scientific name: Streptomyces phaeochromogenes (Conn 1917) Waksman 1957

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19399Brown10-14 daysISP 2
1939910-14 daysISP 3
1939910-14 daysISP 4
1939910-14 daysISP 5
1939910-14 daysISP 6
1939910-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19399yesAerial MyceliumGreyISP 2
19399yesAerial MyceliumGreyISP 3
19399yesAerial MyceliumGreyISP 4
19399yesAerial MyceliumGreyISP 5
19399yesAerial MyceliumWhiteISP 6
19399yesAerial MyceliumGreyISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9271GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://bacmedia.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19399ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19399ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19399ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19399ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19399ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19399ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19399positiveoptimum28mesophilic
9271positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19399
  • spore description: Formation of spore chains: retinaculiapetri, spore surface: smooth, Fragmentation: +
  • spore formation: yes

compound production

@refcompound
9271tyrosinate
20216Tyrosinase
20216Penicillin derivatives

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1939916634raffinose-
1939926546rhamnose+
1939928757fructose+/-
1939929864mannitol-
1939917268myo-inositol-
1939918222xylose-
1939917992sucrose+
1939922599arabinose+
1939917234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19399++---+++-++

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
92711Risk group (German classification)
193991Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces phaeochromogenes strain ATCC 23945 16S ribosomal RNA gene, partial sequenceEU5944681466ena1923
20218Streptomyces phaeochromogenes strain ATCC 3338 16S ribosomal RNA gene, partial sequenceEU5944691475ena1923
20218Streptomyces phaeochromogenes 16S ribosomal RNA gene, partial sequenceAF5000711450ena1923
20218Streptomyces phaeochromogenes gene for 16S ribosomal RNA, partial sequence, strain: JCM 4659D44289120ena1923
20218Streptomyces phaeochromogenes gene for 16S rRNA, partial sequence, strain: NBRC 12898AB1842281476ena1923
20218Streptomyces phaeochromogenes gene for 16S rRNA, partial sequence, strain: NBRC 3180AB1847381479ena1923
20218Streptomyces phaeochromogenes strain NRRL B-1248 16S ribosomal RNA gene, partial sequenceEU5944721492ena1923

Genome sequences

  • @ref: 67770
  • description: Streptomyces phaeochromogenes strain NRRL B-1248, whole genome shotgun sequencing project
  • accession: LIQZ00000000
  • database: ncbi
  • NCBI tax ID: 1923

External links

@ref: 9271

culture collection no.: DSM 40073, ATCC 23945, ATCC 3338, CBS 929.68, ETH 20197, IFO 12898, IMRU 3338, ISP 5073, KCC S-0070, KCC S-0659, NBRC 12898, NCIMB 8505, RIA 1119, JCM 4070, BCRC 12484, CBS 282.30, CBS 288.60, CECT 3070, CGMCC 4.0613, CGMCC 4.1308, IFM 1051, IFO 3180, IMET 40355, JCM 4659, KCTC 9763, LMG 19348, NBRC 3180, NRRL B-1248, NRRL B-3010, RIA 61, VKM Ac-1002, VTT E-82160

straininfo link

@refpassport
20218http://www.straininfo.net/strains/50482
20218http://www.straininfo.net/strains/50476
20218http://www.straininfo.net/strains/50477
20218http://www.straininfo.net/strains/230031
20218http://www.straininfo.net/strains/122107
20218http://www.straininfo.net/strains/50484
20218http://www.straininfo.net/strains/164689
20218http://www.straininfo.net/strains/50487
20218http://www.straininfo.net/strains/50490
20218http://www.straininfo.net/strains/50491
20218http://www.straininfo.net/strains/334053
20218http://www.straininfo.net/strains/50493
20218http://www.straininfo.net/strains/50496

literature

  • topic: Phylogeny
  • Pubmed-ID: 32274558
  • title: Streptomyces albicerus sp. nov., a novel actinomycete isolated from the sediments of the Tailan River in Xinjiang, China.
  • authors: Sun B, Yuan L, Xia Z, Wan C, Zhang L
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-020-01871-6
  • year: 2020
  • mesh: Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers, Species Specificity, Streptomyces/*classification/genetics, Vitamin K 2/chemistry

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9271Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40073)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40073
19399Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40073.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E