Strain identifier

BacDive ID: 15434

Type strain: Yes

Species: Streptomyces olivaceiscleroticus

Strain history: KCC A-0045 <-- T. Furumai <-- IMRU 3751.

NCBI tax ID(s): 68245 (species)

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General

@ref: 9671

BacDive-ID: 15434

DSM-Number: 40595

keywords: 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces olivaceiscleroticus DSM 40595 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 68245
  • Matching level: species

strain history

@refhistory
9671<- E.B. Shirling, ISP <- ATCC <- H.A. Lechevalier, IMRU <- M.J. Thirumalachar
67770KCC A-0045 <-- T. Furumai <-- IMRU 3751.

doi: 10.13145/bacdive15434.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces olivaceiscleroticus
  • full scientific name: Streptomyces olivaceiscleroticus Pridham 1970 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Chainia olivacea

@ref: 9671

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces olivaceiscleroticus

full scientific name: Streptomyces olivaceiscleroticus Pridham 1970

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19482Orange10-14 daysISP 2
19482Orange brown10-14 daysISP 3
19482Orange brown10-14 daysISP 4
19482Yellow10-14 daysISP 5
19482Yellow10-14 daysISP 6
19482Yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19482yesAerial MyceliumWhiteISP 2
19482yesAerial MyceliumLight greyISP 3
19482yesAerial MyceliumWhiteISP 4
19482yesAerial MyceliumWhiteISP 5
19482noISP 6
19482yesAerial MyceliumGreyISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9671GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19482ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19482ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19482ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19482ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19482ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19482ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19482positiveoptimum28mesophilic
9671positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19482
  • spore description: Formation of spore chains (spirales)(Sp), spore surface smooth
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 19482
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1948262968cellulose-
1948216634raffinose+
1948226546rhamnose+/-
1948228757fructose-
1948229864mannitol-
1948217268myo-inositol+/-
1948218222xylose-
1948217992sucrose+
1948222599arabinose-
1948217234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19482++--+-+---+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9671soil
67770SoilPimpriIndiaINDAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
96711Risk group (German classification)
194821Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces olivaceiscleroticus 16S rRNA gene, type strain DSM 40595TAJ6216061498ena68245
20218Streptomyces olivaceiscleroticus strain DSM 40595 16S ribosomal RNA gene, partial sequenceAY9998131422ena68245
20218Streptomyces olivaceiscleroticus gene for 16S rRNA, partial sequenceAB122761565ena68245
20218Streptomyces olivaceiscleroticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4805D44375121ena68245
20218Streptomyces olivaceiscleroticus 16S rRNA gene, type strain LMG 20081AJ7813501481ena68245
20218Streptomyces olivaceiscleroticus gene for 16S rRNA, partial sequence, strain: NBRC 13484AB1844361480ena68245

External links

@ref: 9671

culture collection no.: DSM 40595, ATCC 15722, CBS 785.72, IMRU 3751, ISP 5595, JCM 3045, JCM 4805, RIA 1445, BCRC 11608, CBS 296.66, CGMCC 4.1991, IFO 13484, KCTC 19945, KCTC 19963, LMG 20081, NBRC 13484, NRRL B-2318

straininfo link

  • @ref: 84521
  • straininfo: 297087

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9671Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40595)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40595
19482Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40595.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
84521Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297087.1StrainInfo: A central database for resolving microbial strain identifiers