Strain identifier
BacDive ID: 15434
Type strain:
Species: Streptomyces olivaceiscleroticus
Strain history: KCC A-0045 <-- T. Furumai <-- IMRU 3751.
NCBI tax ID(s): 68245 (species)
General
@ref: 9671
BacDive-ID: 15434
DSM-Number: 40595
keywords: 16S sequence, Bacteria, spore-forming, mesophilic
description: Streptomyces olivaceiscleroticus DSM 40595 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.
NCBI tax id
- NCBI tax id: 68245
- Matching level: species
strain history
@ref | history |
---|---|
9671 | <- E.B. Shirling, ISP <- ATCC <- H.A. Lechevalier, IMRU <- M.J. Thirumalachar |
67770 | KCC A-0045 <-- T. Furumai <-- IMRU 3751. |
doi: 10.13145/bacdive15434.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces olivaceiscleroticus
- full scientific name: Streptomyces olivaceiscleroticus Pridham 1970 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Chainia olivacea
@ref: 9671
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces olivaceiscleroticus
full scientific name: Streptomyces olivaceiscleroticus Pridham 1970
type strain: yes
Morphology
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19482 | Orange | 10-14 days | ISP 2 |
19482 | Orange brown | 10-14 days | ISP 3 |
19482 | Orange brown | 10-14 days | ISP 4 |
19482 | Yellow | 10-14 days | ISP 5 |
19482 | Yellow | 10-14 days | ISP 6 |
19482 | Yellow | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
19482 | yes | Aerial Mycelium | White | ISP 2 |
19482 | yes | Aerial Mycelium | Light grey | ISP 3 |
19482 | yes | Aerial Mycelium | White | ISP 4 |
19482 | yes | Aerial Mycelium | White | ISP 5 |
19482 | no | ISP 6 | ||
19482 | yes | Aerial Mycelium | Grey | ISP 7 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
9671 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
19482 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19482 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19482 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19482 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19482 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19482 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19482 | positive | optimum | 28 | mesophilic |
9671 | positive | growth | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
- @ref: 19482
- spore description: Formation of spore chains (spirales)(Sp), spore surface smooth
- type of spore: spore
- spore formation: yes
halophily
- @ref: 19482
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 2.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
19482 | 62968 | cellulose | - | |
19482 | 16634 | raffinose | + | |
19482 | 26546 | rhamnose | +/- | |
19482 | 28757 | fructose | - | |
19482 | 29864 | mannitol | - | |
19482 | 17268 | myo-inositol | +/- | |
19482 | 18222 | xylose | - | |
19482 | 17992 | sucrose | + | |
19482 | 22599 | arabinose | - | |
19482 | 17234 | glucose | + | |
68368 | 5291 | gelatin | + | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | + | hydrolysis |
68368 | 16947 | citrate | + | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | gelatinase | + | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | + | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | + | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
19482 | + | + | - | - | + | - | + | - | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
9671 | soil | ||||
67770 | Soil | Pimpri | India | IND | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
9671 | 1 | Risk group (German classification) |
19482 | 1 | Risk group (German classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptomyces olivaceiscleroticus 16S rRNA gene, type strain DSM 40595T | AJ621606 | 1498 | ena | 68245 |
20218 | Streptomyces olivaceiscleroticus strain DSM 40595 16S ribosomal RNA gene, partial sequence | AY999813 | 1422 | ena | 68245 |
20218 | Streptomyces olivaceiscleroticus gene for 16S rRNA, partial sequence | AB122761 | 565 | ena | 68245 |
20218 | Streptomyces olivaceiscleroticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4805 | D44375 | 121 | ena | 68245 |
20218 | Streptomyces olivaceiscleroticus 16S rRNA gene, type strain LMG 20081 | AJ781350 | 1481 | ena | 68245 |
20218 | Streptomyces olivaceiscleroticus gene for 16S rRNA, partial sequence, strain: NBRC 13484 | AB184436 | 1480 | ena | 68245 |
External links
@ref: 9671
culture collection no.: DSM 40595, ATCC 15722, CBS 785.72, IMRU 3751, ISP 5595, JCM 3045, JCM 4805, RIA 1445, BCRC 11608, CBS 296.66, CGMCC 4.1991, IFO 13484, KCTC 19945, KCTC 19963, LMG 20081, NBRC 13484, NRRL B-2318
straininfo link
- @ref: 84521
- straininfo: 297087
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
9671 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40595) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40595 | |||
19482 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40595.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
84521 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID297087.1 | StrainInfo: A central database for resolving microbial strain identifiers |