Strain identifier

BacDive ID: 15416

Type strain: Yes

Species: Streptomyces murinus

Strain Designation: Ital. 1131

Strain history: KCC S-0333 <-- H. Nonomura <-- ISP 5091 <-- NRRL B-2286 <-- W. Frommer Ital. 1131.

NCBI tax ID(s): 33900 (species)

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General

@ref: 9303

BacDive-ID: 15416

DSM-Number: 40091

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces murinus Ital. 1131 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 33900
  • Matching level: species

strain history

@refhistory
9303<- E.B. Shirling, ISP <- NRRL <- W. Frommer, Ital. 1131
67770KCC S-0333 <-- H. Nonomura <-- ISP 5091 <-- NRRL B-2286 <-- W. Frommer Ital. 1131.

doi: 10.13145/bacdive15416.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces murinus
  • full scientific name: Streptomyces murinus Frommer 1959 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Streptomyces costaricanus
    20215Streptomyces griseofuscus
    20215Streptomyces phaeogriseichromatogenes

@ref: 9303

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces murinus

full scientific name: Streptomyces murinus Frommer 1959

strain designation: Ital. 1131

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19400Orange10-14 daysISP 2
19400Yellow10-14 daysISP 3
19400Orange10-14 daysISP 4
19400Yellow10-14 daysISP 5
19400Yellow10-14 daysISP 6
19400Yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19400yesAerial MyceliumYellow/GreyISP 2
19400yesAerial MyceliumGreyISP 3
19400yesAerial MyceliumGreyISP 4
19400yesAerial MyceliumWhiteISP 5
19400yesAerial MyceliumGreyISP 6
19400yesAerial MyceliumGreyISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9303GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19400ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19400ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19400ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19400ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19400ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19400ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9303STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf
9303ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
19400positiveoptimum30mesophilic
9303positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19400
  • spore description: Formation of spore chains: retinaculiapetri, spore surface: smooth
  • spore formation: yes

compound production

@refcompound
9303actinomycin X
9303actinomycin D
9303dactinomycin

halophily

  • @ref: 19400
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1940062968cellulose-
1940016634raffinose-
1940026546rhamnose-
1940028757fructose+
1940029864mannitol+
1940017268myo-inositol-
1940018222xylose-
1940017992sucrose-
1940022599arabinose-
1940017234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refmetaboliteproductionChebi-ID
67770actinomycin Xyes
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19400+-----+--++

Isolation, sampling and environmental information

isolation

  • @ref: 9303
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_3145.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_224;98_2413;99_3145&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AB184155
  • Sequence Identity:
  • Total samples: 462
  • soil counts: 384
  • aquatic counts: 10
  • animal counts: 13
  • plant counts: 55

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
93031Risk group (German classification)
194001Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces murinus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4333D44056120ena33900
20218Streptomyces murinus gene for 16S rRNA, partial sequence, strain: NBRC 12799AB1841551476ena33900
20218Streptomyces murinus gene for 16S rRNA, partial sequence, strain: NBRC 14802AB1846221463ena33900
20218Streptomyces murinus strain NRRL B-2286 16S ribosomal RNA gene, partial sequenceDQ0266671364ena33900

Genome sequences

  • @ref: 67770
  • description: Streptomyces murinus NRRL B-2286
  • accession: GCA_002154555
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 33900

GC content

  • @ref: 67770
  • GC-content: 71.7
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.24no
gram-positiveyes90.713no
anaerobicno98.842no
halophileno94.317no
spore-formingyes96.835yes
glucose-utilyes88.286yes
aerobicyes90.791no
thermophileno97.287yes
glucose-fermentno90.965no
motileno94.753no

External links

@ref: 9303

culture collection no.: DSM 40091, ATCC 19788, CBS 540.68, IFO 12799, ISP 5091, JCM 4333, JCM 4595, NBRC 12799, NRRL B-2286, RIA 1067, BCRC 12061, CECT 3309, IFO 14802, KCTC 9492, LMG 10475, NBRC 14802, NCIMB 12701, PCM 2369, VKM Ac-1190

straininfo link

  • @ref: 84504
  • straininfo: 35620

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9303Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40091)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40091
19400Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40091.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
84504Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35620.1StrainInfo: A central database for resolving microbial strain identifiers