Strain identifier

BacDive ID: 15329

Type strain: Yes

Species: Streptomyces intermedius

Strain Designation: Wollenweber

Strain history: KCC S-0197 <-- NRRL B-1327.

NCBI tax ID(s): 53449 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 9288

BacDive-ID: 15329

DSM-Number: 40372

keywords: 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces intermedius Wollenweber is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 53449
  • Matching level: species

strain history

@refhistory
9288<- E.B. Shirling, ISP <- CBS
67770KCC S-0197 <-- NRRL B-1327.

doi: 10.13145/bacdive15329.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces intermedius
  • full scientific name: Streptomyces intermedius (Krüger 1904) Waksman 1953 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Oospora intermedia

@ref: 9288

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces intermedius

full scientific name: Streptomyces intermedius (Krüger 1904) Waksman 1953

strain designation: Wollenweber

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19447Yellowish-brown10-14 daysISP 2
19447Yellow10-14 daysISP 3
19447Yellow10-14 daysISP 4
19447Yellow10-14 daysISP 5

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19447yesAerial MyceliumWhiteISP 2
19447yesAerial MyceliumIvoryISP 3
19447yesAerial MyceliumIvoryISP 4
19447yesAerial MyceliumWhiteISP 5

multimedia

  • @ref: 9288
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40372.jpg
  • caption: Medium 84 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9288GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19447ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19447ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19447ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19447ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
9288ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
19447positiveoptimum30mesophilic
9288positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19447
  • spore description: Formation of spore chains: rectiflixibilis, spore surface: smooth
  • type of spore: spore
  • spore formation: yes

compound production

  • @ref: 20216
  • compound: Heptaenic antifungal activity

halophily

  • @ref: 19447
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1944762968cellulose-
1944716634raffinose-
1944726546rhamnose-
1944728757fructose+/-
1944729864mannitol+
1944717268myo-inositol-
1944718222xylose+/-
1944717992sucrose-
1944722599arabinose+
1944717234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19447----+-----+

Isolation, sampling and environmental information

isolation

  • @ref: 9288
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
92881Risk group (German classification)
194471Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218S.intermedius (DSM 40372T) 16S rRNA geneZ766861474ena53449
20218Streptomyces intermedius gene for 16S ribosomal RNA, partial sequence, strain: JCM 4483D44178120ena53449
20218Streptomyces intermedius gene for 16S rRNA, partial sequence, strain: NBRC 13049AB1842771475ena53449
20218Streptomyces intermedius 16S ribosomal RNA gene, partial sequenceKC355423817ena53449

GC content

  • @ref: 67770
  • GC-content: 71
  • method: fluorimetric

External links

@ref: 9288

culture collection no.: DSM 40372, ATCC 3329, CBS 101.21, CBS 694.69, IFO 13049, ISP 5372, NBRC 13049, RIA 1241, JCM 4197, ATCC 25461, BCRC 13706, CGMCC 4.1467, ICMP 12540, IMET 41384, JCM 4483, LMG 19304, NRRL B-1327, NRRL B-2670

straininfo link

  • @ref: 84422
  • straininfo: 92626

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny24002610Streptomyces polyrhachii sp. nov., a novel actinomycete isolated from an edible Chinese black ant (Polyrhachis vicina Roger).Yu C, Liu C, Wang X, Zhao J, Yang L, Gao R, Zhang Y, Xiang WAntonie Van Leeuwenhoek10.1007/s10482-013-0021-32013Animals, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Hymenoptera/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/*isolation & purification/metabolismGenetics
Phylogeny26449519Streptomyces alfalfae sp. nov. and comparisons with its closest taxa Streptomyces silaceus, Streptomyces flavofungini and Streptomyces intermedius.She W, Sun Z, Yi L, Zhao S, Liang YInt J Syst Evol Microbiol10.1099/ijsem.0.0006712015Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Medicago/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny29537363Streptomyces fuscigenes sp. nov., isolated from bamboo (Sasa borealis) litter.Lee HJ, Whang KSInt J Syst Evol Microbiol10.1099/ijsem.0.0027062018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sasa/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9288Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40372)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40372
19447Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40372.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
84422Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92626.1StrainInfo: A central database for resolving microbial strain identifiers