Strain identifier
BacDive ID: 15329
Type strain:
Species: Streptomyces intermedius
Strain Designation: Wollenweber
Strain history: KCC S-0197 <-- NRRL B-1327.
NCBI tax ID(s): 53449 (species)
General
@ref: 9288
BacDive-ID: 15329
DSM-Number: 40372
keywords: 16S sequence, Bacteria, spore-forming, mesophilic
description: Streptomyces intermedius Wollenweber is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.
NCBI tax id
- NCBI tax id: 53449
- Matching level: species
strain history
@ref | history |
---|---|
9288 | <- E.B. Shirling, ISP <- CBS |
67770 | KCC S-0197 <-- NRRL B-1327. |
doi: 10.13145/bacdive15329.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces intermedius
- full scientific name: Streptomyces intermedius (Krüger 1904) Waksman 1953 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Oospora intermedia
@ref: 9288
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces intermedius
full scientific name: Streptomyces intermedius (Krüger 1904) Waksman 1953
strain designation: Wollenweber
type strain: yes
Morphology
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19447 | Yellowish-brown | 10-14 days | ISP 2 |
19447 | Yellow | 10-14 days | ISP 3 |
19447 | Yellow | 10-14 days | ISP 4 |
19447 | Yellow | 10-14 days | ISP 5 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
19447 | yes | Aerial Mycelium | White | ISP 2 |
19447 | yes | Aerial Mycelium | Ivory | ISP 3 |
19447 | yes | Aerial Mycelium | Ivory | ISP 4 |
19447 | yes | Aerial Mycelium | White | ISP 5 |
multimedia
- @ref: 9288
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40372.jpg
- caption: Medium 84 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
9288 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
19447 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19447 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19447 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19447 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
9288 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19447 | positive | optimum | 30 | mesophilic |
9288 | positive | growth | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
- @ref: 19447
- spore description: Formation of spore chains: rectiflixibilis, spore surface: smooth
- type of spore: spore
- spore formation: yes
compound production
- @ref: 20216
- compound: Heptaenic antifungal activity
halophily
- @ref: 19447
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 2.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
19447 | 62968 | cellulose | - | |
19447 | 16634 | raffinose | - | |
19447 | 26546 | rhamnose | - | |
19447 | 28757 | fructose | +/- | |
19447 | 29864 | mannitol | + | |
19447 | 17268 | myo-inositol | - | |
19447 | 18222 | xylose | +/- | |
19447 | 17992 | sucrose | - | |
19447 | 22599 | arabinose | + | |
19447 | 17234 | glucose | + | |
68368 | 5291 | gelatin | + | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | - | hydrolysis |
68368 | 16947 | citrate | + | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | - | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | gelatinase | + | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | - | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
19447 | - | - | - | - | + | - | - | - | - | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 9288
- sample type: soil
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
9288 | 1 | Risk group (German classification) |
19447 | 1 | Risk group (German classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | S.intermedius (DSM 40372T) 16S rRNA gene | Z76686 | 1474 | ena | 53449 |
20218 | Streptomyces intermedius gene for 16S ribosomal RNA, partial sequence, strain: JCM 4483 | D44178 | 120 | ena | 53449 |
20218 | Streptomyces intermedius gene for 16S rRNA, partial sequence, strain: NBRC 13049 | AB184277 | 1475 | ena | 53449 |
20218 | Streptomyces intermedius 16S ribosomal RNA gene, partial sequence | KC355423 | 817 | ena | 53449 |
GC content
- @ref: 67770
- GC-content: 71
- method: fluorimetric
External links
@ref: 9288
culture collection no.: DSM 40372, ATCC 3329, CBS 101.21, CBS 694.69, IFO 13049, ISP 5372, NBRC 13049, RIA 1241, JCM 4197, ATCC 25461, BCRC 13706, CGMCC 4.1467, ICMP 12540, IMET 41384, JCM 4483, LMG 19304, NRRL B-1327, NRRL B-2670
straininfo link
- @ref: 84422
- straininfo: 92626
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 24002610 | Streptomyces polyrhachii sp. nov., a novel actinomycete isolated from an edible Chinese black ant (Polyrhachis vicina Roger). | Yu C, Liu C, Wang X, Zhao J, Yang L, Gao R, Zhang Y, Xiang W | Antonie Van Leeuwenhoek | 10.1007/s10482-013-0021-3 | 2013 | Animals, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Hymenoptera/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/*isolation & purification/metabolism | Genetics |
Phylogeny | 26449519 | Streptomyces alfalfae sp. nov. and comparisons with its closest taxa Streptomyces silaceus, Streptomyces flavofungini and Streptomyces intermedius. | She W, Sun Z, Yi L, Zhao S, Liang Y | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000671 | 2015 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Medicago/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Phylogeny | 29537363 | Streptomyces fuscigenes sp. nov., isolated from bamboo (Sasa borealis) litter. | Lee HJ, Whang KS | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002706 | 2018 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sasa/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
9288 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40372) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40372 | |||
19447 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40372.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20216 | Curators of the HKI | http://www.leibniz-hki.de/de/ | Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
84422 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92626.1 | StrainInfo: A central database for resolving microbial strain identifiers |