Strain identifier

BacDive ID: 15309

Type strain: Yes

Species: Streptomyces hydrogenans

Strain Designation: FHP 678

Strain history: KCC S-0771 <-- IFO 13475 <-- SAJ <-- ISP 5586 <-- ATCC 19631 <-- G. Nesemann FHP 678.

NCBI tax ID(s): 1873719 (species)

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General

@ref: 9663

BacDive-ID: 15309

DSM-Number: 40586

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces hydrogenans FHP 678 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1873719
  • Matching level: species

strain history

@refhistory
9663<- E.B. Shirling, ISP <- ATCC <- G. Nesemann, FHP 678
67770KCC S-0771 <-- IFO 13475 <-- SAJ <-- ISP 5586 <-- ATCC 19631 <-- G. Nesemann FHP 678.

doi: 10.13145/bacdive15309.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces hydrogenans
  • full scientific name: Streptomyces hydrogenans Lindner et al. 1958 (Approved Lists 1980)

@ref: 9663

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces hydrogenans

full scientific name: Streptomyces hydrogenans Lindner et al. 1958

strain designation: FHP 678

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no91.938
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
19479Ivory10-14 daysISP 2
19479Ivory10-14 daysISP 3
19479Ivory10-14 daysISP 4
19479Yellow10-14 daysISP 5

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19479yesAerial MyceliumGreyISP 2
19479yesAerial MyceliumGreyISP 3
19479yesAerial MyceliumGreyISP 4
19479yesAerial MyceliumWhite/ yellowISP 5
19479noISP 6
19479noISP 7

multimedia

  • @ref: 9663
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40586.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9663GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19479ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19479ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19479ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19479ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19479positiveoptimum30mesophilic
9663positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

tolerance

  • @ref: 19479
  • compound: Lysozyme
  • percentage: 0.1

spore formation

@refspore descriptiontype of sporespore formationconfidence
19479Formation of spore chains (rectiflixibilis), spore surface smooth, fragmentation in aerial myceliumsporeyes
69481yes100
69480yes100

compound production

@refcompound
966320-ß keto-reductase
966320-ß hydroxysteroid dehydrogenase
9663ketoses
9663steroids
2021620-ß-keto reductase
2021620-ß-steroid dehydrogenase

halophily

  • @ref: 19479
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1947962968cellulose-
1947916634raffinose-
1947926546rhamnose-
1947928757fructose-
1947929864mannitol-
1947917268myo-inositol-
1947918222xylose-
1947917992sucrose-
1947922599arabinose+
1947917234glucose+

Isolation, sampling and environmental information

isolation

  • @ref: 9663
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
96631Risk group (German classification)
194791Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces exfoliatus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4771D44348120ena1905
20218Streptomyces exfoliatus gene for 16S rRNA, partial sequence, strain: NBRC 13475AB1848681452ena1905
20218Streptomyces sp. NTRHn16 gene for 16S ribosomal RNA, partial sequenceAB9205971280ena1571733
20218Streptomyces sp. NTS4 gene for 16S ribosomal RNA, partial sequenceAB9206001271ena1571736
20218Streptomyces sp. NTS3 gene for 16S ribosomal RNA, partial sequenceAB9206051302ena1571741
20218Streptomyces exfoliatus gene for 16S ribosomal RNA, partial sequence, isolate: R1-1A/A106AB9228381173ena1905
67770Streptomyces exfoliatus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4771AB9794461465ena1905

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces hydrogenans JCM 4771GCA_014656075scaffoldncbi1873719
66792Streptomyces hydrogenans NBRC 13475GCA_020521255contigncbi1873719
66792Streptomyces hydrogenans strain JCM 47711873719.3wgspatric1873719
66792Streptomyces hydrogenans strain NBRC 134751873719.6wgspatric1873719
66792Streptomyces hydrogenans strain NBRC 134751873719.4wgspatric1873719
66792Streptomyces hydrogenans strain NBRC 134751873719.5wgspatric1873719

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno94.204no
flagellatedno97.59no
gram-positiveyes88.721no
anaerobicno99.342no
aerobicyes93.15no
halophileno94.396no
spore-formingyes94.843no
glucose-utilyes88.67yes
thermophileno99.189no
glucose-fermentno85.297no

External links

@ref: 9663

culture collection no.: DSM 40586, ATCC 19631, CBS 776.72, IFO 13475, ISP 5586, NBRC 13475, RIA 1436, JCM 4771, BCRC 11855, HAMBI 405, NRRL B-12091, VKM Ac-1919

straininfo link

  • @ref: 84402
  • straininfo: 45630

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity852821Enzyme induction in Streptomyces hydrogenas, VII. Short-term accumulation of guanosine polyphosphates.Betz JW, Schneider BH, Trager LHoppe Seylers Z Physiol Chem10.1515/bchm2.1977.358.1.3531977Adenosine Triphosphate/metabolism, Cell Division/drug effects, Chromatography, Thin Layer, DNA Replication/drug effects, Guanine Nucleotides/*metabolism, Guanosine Triphosphate/metabolism, Kinetics, Pregnadienediols/pharmacology, Protein Biosynthesis/drug effects, Streptomyces/drug effects/*metabolism, Testosterone/pharmacology, Transcription, Genetic/drug effectsMetabolism
Phylogeny27566709Streptomyces atlanticus sp. nov., a novel actinomycete isolated from marine sponge Aplysina fulva (Pallas, 1766).Silva FS, Souza DT, Zucchi TD, Pansa CC, de Figueiredo Vasconcellos RL, Crevelin EJ, de Moraes LA, Melo ISAntonie Van Leeuwenhoek10.1007/s10482-016-0748-82016Animals, Molecular Typing, Phylogeny, Porifera/*microbiology, RNA, Bacterial, RNA, Ribosomal, 16S/genetics, Streptomyces/classification/*isolation & purificationEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9663Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40586)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40586
19479Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40586.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84402Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45630.1StrainInfo: A central database for resolving microbial strain identifiers