Strain identifier

BacDive ID: 15290

Type strain: Yes

Species: Streptomyces hawaiiensis

Strain history: KCC S-0172 <-- IFM 1071 <-- Bristol Labs.; 678506 (A8506).

NCBI tax ID(s): 67305 (species)

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General

@ref: 9121

BacDive-ID: 15290

DSM-Number: 40042

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces hawaiiensis DSM 40042 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 67305
  • Matching level: species

strain history

@refhistory
9121<- E.B. Shirling, ISP <- K.E. Crook, Bristol Labs, 678 506
67770KCC S-0172 <-- IFM 1071 <-- Bristol Labs.; 678506 (A8506).

doi: 10.13145/bacdive15290.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces hawaiiensis
  • full scientific name: Streptomyces hawaiiensis Cron et al. 1956 (Approved Lists 1980)

@ref: 9121

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces hawaiiensis

full scientific name: Streptomyces hawaiiensis Cron et al. 1956

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no94.051
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
19393Yellow/brown10-14 daysISP 2
19393Brown10-14 daysISP 3
19393Brown10-14 daysISP 4
19393Brown10-14 daysISP 5

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19393yesAerial MyceliumBlue/GreyISP 2
19393yesAerial MyceliumGreyISP 3
19393yesAerial MyceliumBlue/GreyISP 4
19393yesAerial MyceliumWhiteISP 5

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9121GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19393ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19393ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19393ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19393ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
9121ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19393positiveoptimum28mesophilic
9121positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

tolerance

  • @ref: 19393
  • compound: Lysozyme
  • percentage: 1

spore formation

@refspore descriptionspore formationconfidence
19393Formation of spore chains: spira, spore surface: spinyyes
69481yes100
69480yes100

compound production

@refcompound
9121bryamycin
20216Bryamycin = Thiostrepton

halophily

  • @ref: 19393
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1939316634raffinose+
1939326546rhamnose+
1939328757fructose+
1939329864mannitol+
1939317268myo-inositol+
1939318222xylose-
1939317992sucrose-
1939322599arabinose+
1939317234glucose+
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refmetaboliteproductionChebi-ID
67770bryamycinyes
68368acetoinno15688
68368indoleno35581
68368hydrogen sulfideno16136

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase+4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19393+------+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9121soilHawaiiUSAUSANorth America
67770SoilHIUSAUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
91211Risk group (German classification)
193931Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces hawaiiensis partial 16S rRNA gene, strain ISP 5042AJ3994661448ena67305
20218Streptomyces hawaiiensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 4585D44248121ena67305
20218Streptomyces hawaiiensis gene for 16S rRNA, partial sequence, strain: NBRC 12784AB1841431477ena67305

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces hawaiiensis ATCC 12236GCA_004803895completencbi67305
66792Streptomyces hawaiiensis strain ATCC 1223667305.10completepatric67305

GC content

  • @ref: 67770
  • GC-content: 72.7
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno93.808no
flagellatedno97.61no
gram-positiveyes88.966no
anaerobicno98.95no
aerobicyes92.339no
halophileno93.237no
spore-formingyes96.307yes
glucose-utilyes89.396no
thermophileno99.045no
glucose-fermentno89.534no

External links

@ref: 9121

culture collection no.: DSM 40042, ATCC 12236, ATCC 19771, CBS 509.68, IFO 12784, ISP 5042, KCC S-0172, KCC S-0585, NBRC 12784, NCIB 9410, RIA 1051, JCM 4172, BCRC 13653, CBS 164.64, IFM 1071, IMET 43082, JCM 4585, LMG 5975, NCIMB 9410, NRRL B-1988, PCM 2315, VKM Ac-1761

straininfo link

  • @ref: 84384
  • straininfo: 13327

literature

  • topic: Phylogeny
  • Pubmed-ID: 28141499
  • title: Streptomyces jeddahensis sp. nov., an oleaginous bacterium isolated from desert soil.
  • authors: Rottig A, Atasayar E, Meier-Kolthoff JP, Sproer C, Schumann P, Schauer J, Steinbuchel A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001839
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saudi Arabia, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9121Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40042)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40042
19393Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40042.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84384Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13327.1StrainInfo: A central database for resolving microbial strain identifiers