Strain identifier
BacDive ID: 15290
Type strain:
Species: Streptomyces hawaiiensis
Strain history: KCC S-0172 <-- IFM 1071 <-- Bristol Labs.; 678506 (A8506).
NCBI tax ID(s): 67305 (species)
General
@ref: 9121
BacDive-ID: 15290
DSM-Number: 40042
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production
description: Streptomyces hawaiiensis DSM 40042 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.
NCBI tax id
- NCBI tax id: 67305
- Matching level: species
strain history
@ref | history |
---|---|
9121 | <- E.B. Shirling, ISP <- K.E. Crook, Bristol Labs, 678 506 |
67770 | KCC S-0172 <-- IFM 1071 <-- Bristol Labs.; 678506 (A8506). |
doi: 10.13145/bacdive15290.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces hawaiiensis
- full scientific name: Streptomyces hawaiiensis Cron et al. 1956 (Approved Lists 1980)
@ref: 9121
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces hawaiiensis
full scientific name: Streptomyces hawaiiensis Cron et al. 1956
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 94.051 | |
69480 | 100 | positive |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19393 | Yellow/brown | 10-14 days | ISP 2 |
19393 | Brown | 10-14 days | ISP 3 |
19393 | Brown | 10-14 days | ISP 4 |
19393 | Brown | 10-14 days | ISP 5 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
19393 | yes | Aerial Mycelium | Blue/Grey | ISP 2 |
19393 | yes | Aerial Mycelium | Grey | ISP 3 |
19393 | yes | Aerial Mycelium | Blue/Grey | ISP 4 |
19393 | yes | Aerial Mycelium | White | ISP 5 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
9121 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
19393 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19393 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19393 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19393 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
9121 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19393 | positive | optimum | 28 | mesophilic |
9121 | positive | growth | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
tolerance
- @ref: 19393
- compound: Lysozyme
- percentage: 1
spore formation
@ref | spore description | spore formation | confidence |
---|---|---|---|
19393 | Formation of spore chains: spira, spore surface: spiny | yes | |
69481 | yes | 100 | |
69480 | yes | 100 |
compound production
@ref | compound |
---|---|
9121 | bryamycin |
20216 | Bryamycin = Thiostrepton |
halophily
- @ref: 19393
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
19393 | 16634 | raffinose | + | |
19393 | 26546 | rhamnose | + | |
19393 | 28757 | fructose | + | |
19393 | 29864 | mannitol | + | |
19393 | 17268 | myo-inositol | + | |
19393 | 18222 | xylose | - | |
19393 | 17992 | sucrose | - | |
19393 | 22599 | arabinose | + | |
19393 | 17234 | glucose | + | |
68368 | 5291 | gelatin | - | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | - | hydrolysis |
68368 | 16947 | citrate | - | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | - | hydrolysis |
metabolite production
@ref | metabolite | production | Chebi-ID |
---|---|---|---|
67770 | bryamycin | yes | |
68368 | acetoin | no | 15688 |
68368 | indole | no | 35581 |
68368 | hydrogen sulfide | no | 16136 |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | gelatinase | - | |
68368 | tryptophan deaminase | + | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
19393 | + | - | - | - | - | - | - | + | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
9121 | soil | Hawaii | USA | USA | North America |
67770 | Soil | HI | USA | USA | North America |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
9121 | 1 | Risk group (German classification) |
19393 | 1 | Risk group (German classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptomyces hawaiiensis partial 16S rRNA gene, strain ISP 5042 | AJ399466 | 1448 | ena | 67305 |
20218 | Streptomyces hawaiiensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 4585 | D44248 | 121 | ena | 67305 |
20218 | Streptomyces hawaiiensis gene for 16S rRNA, partial sequence, strain: NBRC 12784 | AB184143 | 1477 | ena | 67305 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Streptomyces hawaiiensis ATCC 12236 | GCA_004803895 | complete | ncbi | 67305 |
66792 | Streptomyces hawaiiensis strain ATCC 12236 | 67305.10 | complete | patric | 67305 |
GC content
- @ref: 67770
- GC-content: 72.7
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | no | 93.808 | no |
flagellated | no | 97.61 | no |
gram-positive | yes | 88.966 | no |
anaerobic | no | 98.95 | no |
aerobic | yes | 92.339 | no |
halophile | no | 93.237 | no |
spore-forming | yes | 96.307 | yes |
glucose-util | yes | 89.396 | no |
thermophile | no | 99.045 | no |
glucose-ferment | no | 89.534 | no |
External links
@ref: 9121
culture collection no.: DSM 40042, ATCC 12236, ATCC 19771, CBS 509.68, IFO 12784, ISP 5042, KCC S-0172, KCC S-0585, NBRC 12784, NCIB 9410, RIA 1051, JCM 4172, BCRC 13653, CBS 164.64, IFM 1071, IMET 43082, JCM 4585, LMG 5975, NCIMB 9410, NRRL B-1988, PCM 2315, VKM Ac-1761
straininfo link
- @ref: 84384
- straininfo: 13327
literature
- topic: Phylogeny
- Pubmed-ID: 28141499
- title: Streptomyces jeddahensis sp. nov., an oleaginous bacterium isolated from desert soil.
- authors: Rottig A, Atasayar E, Meier-Kolthoff JP, Sproer C, Schumann P, Schauer J, Steinbuchel A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001839
- year: 2017
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saudi Arabia, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
9121 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40042) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40042 | |||
19393 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40042.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20216 | Curators of the HKI | http://www.leibniz-hki.de/de/ | Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
84384 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID13327.1 | StrainInfo: A central database for resolving microbial strain identifiers |