Strain identifier

BacDive ID: 1526

Type strain: No

Species: Lysinibacillus sphaericus

Strain Designation: 9602

Strain history: CIP <- 2009, J.F. Charles, Inst. Pasteur, Paris, France <- 2001, B. Papierok, Inst. Pasteur, Paris, France <- 1998, M.M. Lecadet, Inst. Pasteur, Paris, France <- 1992, H. de Barjac, Inst. Pasteur, Paris, France <- 1978, A.A. Yousten: strain NCTC 9602

NCBI tax ID(s): 1421 (species)

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General

@ref: 178

BacDive-ID: 1526

DSM-Number: 396

keywords: 16S sequence, Bacteria, mesophilic, human pathogen

description: Lysinibacillus sphaericus 9602 is a mesophilic human pathogen that was isolated from air.

NCBI tax id

  • NCBI tax id: 1421
  • Matching level: species

strain history

@refhistory
178<- L. Landbeck <- D.J. Tipper, 9602 <- A. Warth <- J.F. Powell <- NCTC <- W.R. Bale
123121CIP <- 2009, J.F. Charles, Inst. Pasteur, Paris, France <- 2001, B. Papierok, Inst. Pasteur, Paris, France <- 1998, M.M. Lecadet, Inst. Pasteur, Paris, France <- 1992, H. de Barjac, Inst. Pasteur, Paris, France <- 1978, A.A. Yousten: strain NCTC 9602

doi: 10.13145/bacdive1526.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Lysinibacillus
  • species: Lysinibacillus sphaericus
  • full scientific name: Lysinibacillus sphaericus (Meyer and Neide 1904) Ahmed et al. 2007

  • synonyms

    @refsynonym
    20215Lysinibacillus varians
    20215Bacillus sphaericus
    20215Lysinibacillus mangiferahumi
    20215Lysinibacillus mangiferihumi
    20215Lysinibacillus tabacifolii

@ref: 178

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Caryophanaceae

genus: Lysinibacillus

species: Lysinibacillus sphaericus

full scientific name: Lysinibacillus sphaericus (Meyer and Neide 1904) Ahmed et al. 2007

strain designation: 9602

type strain: no

Culture and growth conditions

culture medium

  • @ref: 178
  • name: NUTRIENT AGAR (DSMZ Medium 1)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1
  • composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 178
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

compound production

@refcompound
178disulfide reductase
178L leucine dehydrogenase
178Leucine dehydrogenase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12312117234glucose-fermentation
1231212759inuline-fermentation
12312117716lactose-fermentation
12312117632nitrate-reduction
12312118222xylose-fermentation

metabolite tests

  • @ref: 123121
  • Chebi-ID: 17234
  • metabolite: glucose
  • methylred-test: -

enzymes

@refvalueactivityec
123121lecithinase-
123121urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
178air
123121United States of AmericaUSANorth America1978

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Air

taxonmaps

  • @ref: 69479
  • File name: preview.99_4772.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_503;97_577;98_664;99_4772&stattab=map
  • Last taxonomy: Lysinibacillus
  • 16S sequence: AJ311894
  • Sequence Identity:
  • Total samples: 337
  • soil counts: 72
  • aquatic counts: 49
  • animal counts: 210
  • plant counts: 6

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
178yes, in single casesyes1Risk group (German classification)
1231211Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Bacillus sphaericus partial 16S rRNA gene, strain DSM 396, NTCC 9602
  • accession: AJ311894
  • length: 1563
  • database: ena
  • NCBI tax ID: 1421

GC content

  • @ref: 178
  • GC-content: 36.0

External links

@ref: 178

culture collection no.: DSM 396, NCTC 9602, CRBIP3.5052

straininfo link

  • @ref: 71172
  • straininfo: 47162

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology3922755Purification and partial characterization of the extracellular gamma-D-glutamyl-(L)meso-diaminopimelate endopeptidase I, from Bacillus sphaericus NCTC 9602.Garnier M, Vacheron MJ, Guinand M, Michel GEur J Biochem10.1111/j.1432-1033.1985.tb08873.x1985Bacillus/*enzymology, Drug Stability, Edetic Acid/pharmacology, Endopeptidases/*isolation & purification, Enzyme Activation, Hydrogen-Ion Concentration, Isoelectric Focusing, Metals, Phenanthrolines/pharmacology, Protein Denaturation, Substrate SpecificityPhylogeny
Enzymology8503890Characterization of the sporulation-related gamma-D-glutamyl-(L)meso-diaminopimelic-acid-hydrolysing peptidase I of Bacillus sphaericus NCTC 9602 as a member of the metallo(zinc) carboxypeptidase A family. Modular design of the protein.Hourdou ML, Guinand M, Vacheron MJ, Michel G, Denoroy L, Duez C, Englebert S, Joris B, Weber G, Ghuysen JMBiochem J10.1042/bj29205631993Amino Acid Sequence, Animals, Bacillus/*enzymology/physiology, Base Sequence, Carboxypeptidases/*classification/genetics/metabolism, Carboxypeptidases A, Cattle, Cloning, Molecular, DNA, Bacterial, Detergents, Endopeptidases/*classification/genetics/metabolism, Genes, Bacterial, Molecular Sequence Data, Peptide Mapping, Sequence Homology, Amino Acid, Spectrum Analysis, Spores, Bacterial, Zinc/*metabolismMetabolism
15157620Electron holography of non-stained bacterial surface layer proteins.Simon P, Lichte H, Wahl R, Mertig M, Pompe WBiochim Biophys Acta10.1016/j.bbamem.2004.03.0052004Bacillus/chemistry/cytology/genetics, Bacterial Outer Membrane Proteins/radiation effects/*ultrastructure, Dose-Response Relationship, Radiation, Holography/*methods, Image Processing, Computer-Assisted, Microscopy, Electron
Metabolism16151207Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements.Pollmann K, Raff J, Schnorpfeil M, Radeva G, Selenska-Pobell SMicrobiology (Reading)10.1099/mic.0.28201-02005Bacillus/*genetics/growth & development/*metabolism, Bacterial Proteins/chemistry/genetics/*metabolism, Binding Sites, DNA Transposable Elements/*physiology, Gene Transfer, Horizontal, Genes, Bacterial, Membrane Glycoproteins/chemistry/genetics/*metabolism, Molecular Sequence Data, Transposases/geneticsGenetics
Enzymology16698775Secondary structure and Pd(II) coordination in S-layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy.Fahmy K, Merroun M, Pollmann K, Raff J, Savchuk O, Hennig C, Selenska-Pobell SBiophys J10.1529/biophysj.105.0791372006Bacillus/*enzymology, Bacterial Proteins/*chemistry, Binding Sites, Metals/chemistry, Palladium/*chemistry, Protein Structure, Secondary, Serine Endopeptidases/pharmacology, Spectrometry, X-Ray Emission/*methods, Spectrophotometry, Spectrophotometry, Infrared, Spectroscopy, Fourier Transform Infrared/methods
16853396Photoemission and near-edge X-ray absorption fine structure studies of the bacterial surface protein layer of Bacillus sphaericus NCTC 9602.Vyalikh DV, Kirchner A, Danzenbacher S, Dedkov YS, Kade A, Mertig M, Molodtsov SLJ Phys Chem B10.1021/jp050748+2005Amino Acids/chemistry, Bacillus/*chemistry, Bacterial Proteins/*chemistry, Protein Conformation, Spectrum Analysis/*methods
17997540X-ray absorption microscopy of bacterial surface protein layers: X-ray damage.Kade A, Vyalikh DV, Danzenbacher S, Kummer K, Bluher A, Mertig M, Lanzara A, Scholl A, Doran A, Molodtsov SLJ Phys Chem B10.1021/jp073650z2007Bacterial Proteins/*analysis/radiation effects, Microscopy, Electron/*methods, X-Rays
Metabolism19392567Charge transport in proteins probed by resonant photoemission.Vyalikh DV, Maslyuk VV, Bluher A, Kade A, Kummer K, Dedkov YS, Bredow T, Mertig I, Mertig M, Molodtsov SLPhys Rev Lett10.1103/PhysRevLett.102.0981012009Bacillus/chemistry/metabolism, Bacterial Proteins/*chemistry/metabolism, Membrane Glycoproteins/*chemistry/metabolism, Models, Molecular, Spectrometry, X-Ray Emission/methods
Enzymology22266829Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification.Cui YB, Zhou Y, Liu WN, Chen QW, Ma GF, Shi WH, Wang YG, Yang LInt J Mol Med10.3892/ijmm.2012.8902012Amino Acid Sequence, Bacillus/*genetics/metabolism, Bacterial Proteins/*genetics/metabolism, Blotting, Western, *Cloning, Molecular, DNA Primers, DNA, Bacterial/genetics, Escherichia coli/genetics/isolation & purification, Gene Expression Regulation, Bacterial, Membrane Glycoproteins/*genetics/metabolism, Microscopy, Electron, Transmission, Molecular Sequence Data, Plasmids, Recombinant Proteins/genetics/metabolism, Sequence Analysis, DNAMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
178Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 396)https://www.dsmz.de/collection/catalogue/details/culture/DSM-396
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71172Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47162.1StrainInfo: A central database for resolving microbial strain identifiers
123121Curators of the CIPCollection of Institut Pasteur (CRBIP3.5052)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CRBIP3.5052