Strain identifier

BacDive ID: 15257

Type strain: Yes

Species: Streptomyces griseocarneus

Strain Designation: NA232-M1

Strain history: CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- NRRL <- R.G. Benedict: strain NA232-M1

NCBI tax ID(s): 51201 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9209

BacDive-ID: 15257

DSM-Number: 40004

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces griseocarneus NA232-M1 is an obligate aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 51201
  • Matching level: species

strain history

@refhistory
9209<- E.B. Shirling, ISP <- NRRL <- R. G. Benedict, NA 232-M1
67770KCC S-0095 <-- Y. Okami <-- NRRL B-1068 <-- R. G. Benedict NA232-M1.
119745CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- NRRL <- R.G. Benedict: strain NA232-M1

doi: 10.13145/bacdive15257.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces griseocarneus
  • full scientific name: Streptomyces griseocarneus (Benedict et al. 1950) Witt and Stackebrandt 1991
  • synonyms

    @refsynonym
    20215Streptomyces griseocarneus
    20215Streptoverticillium griseocarneum
    20215Streptoverticillium septatum
    20215Streptoverticillium alboverticillatum
    20215Streptomyces septatus
    20215Streptomyces alboverticillatus

@ref: 9209

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces griseocarneus

full scientific name: Streptomyces griseocarneus (Benedict et al. 1950) Witt and Stackebrandt 1991

strain designation: NA232-M1

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no93.583
69480100positive
119745nopositiverod-shaped

colony morphology

@refcolony colormedium usedhemolysis ability
69336Beige (1001), Ochre yellow (1024)ISP 6
69336Beige (1001), Olive brown (8008)ISP 3
69336Ochre yellow (1024)suter with tyrosine
69336Ochre yellow (1024)suter without tyrosine
69336Ochre yellow (1024), Grey beige (1019)ISP 2
69336Ochre yellow (1024), Grey beige (1019)ISP 7
69336Ochre yellow (1024), Olive brown (8008)ISP 4
69336Sand yellow (1002), Ochre yellow (1024)ISP 5
1197451

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69336yesAerial myceliumPure white (8010)ISP 2
69336yesAerial myceliumSignal white (9003)ISP 3
69336yesAerial myceliumCream (9001)ISP 4
69336yesAerial myceliumOyster white (1013)ISP 5
69336noAerial myceliumISP 6
69336yesAerial myceliumOyster white (1013)ISP 7
69336yesAerial myceliumLight ivory (1015)suter with tyrosine
69336yesAerial myceliumOyster white (1013)suter without tyrosine

pigmentation

@refproductionnamecolor
69336noMelanin
69336yessoluble pigmentSand yellow (1002), ochre brown (8001)

multimedia

@refmultimedia contentcaptionintellectual property rights
69336DSM_40004_image5.jpeg(ISP6, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69336DSM_40004_image6.jpeg(ISP6, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9209GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
32896MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
9209ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
119745CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
18462positiveoptimum28mesophilic
9209positivegrowth37mesophilic
32896positivegrowth30mesophilic
67770positivegrowth28mesophilic
119745positivegrowth25-41
119745nogrowth10psychrophilic
119745nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119745
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100

compound production

@refcompound
9209anti-fungal antibiotic
9209hydroxy streptomycin
9209hydroxystreptomycin
9209aminoacylase
9209rotaventin
20216Aminocyclase
20216Hydroxystreptomycin

halophily

@refsaltgrowthtested relationconcentration
69336NaClpositivegrowth0-2.5 %
119745NaClpositivegrowth0-6 %
119745NaClnogrowth8 %
119745NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6933622599arabinose-growth
6933662968cellulose-growth
6933628757fructose-growth
6933617234glucose+growth
6933617268inositol+/-growth
6933637684mannose-growth
6933616634raffinose-growth
6933626546rhamnose-growth
6933617992sucrose-growth
6933618222xylose-growth
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
11974516947citrate-carbon source
1197454853esculin-hydrolysis
119745606565hippurate+hydrolysis
11974517632nitrate-reduction
11974516301nitrite-reduction
11974517632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 119745
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6777024750hydroxystreptomycinyes
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11974535581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
11974515688acetoin-
11974517234glucose-

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
119745oxidase-
119745beta-galactosidase+3.2.1.23
119745alcohol dehydrogenase-1.1.1.1
119745gelatinase+
119745amylase+
119745DNase+
119745caseinase+3.4.21.50
119745catalase+1.11.1.6
119745tween esterase+
119745gamma-glutamyltransferase+2.3.2.2
119745lecithinase+
119745lipase-
119745lysine decarboxylase-4.1.1.18
119745ornithine decarboxylase-4.1.1.17
119745phenylalanine ammonia-lyase-4.3.1.24
119745tryptophan deaminase-
119745urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69336---+------+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
69336+++-+++/-+-++---+/--++-
119745+++-++---++---+-++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18462+++++-----+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119745+--++----++--------+-----------++---------------++---------++-----------++++---++---+---++++-++-+++

Isolation, sampling and environmental information

isolation

@refsample type
9209soil
67770Soil
119745Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_83390.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8009;96_34893;97_44268;98_57887;99_83390&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AB920588
  • Sequence Identity:
  • Total samples: 178
  • soil counts: 79
  • aquatic counts: 19
  • animal counts: 56
  • plant counts: 24

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
92091Risk group (German classification)
184621German classification
1197451Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces sp. NTRH3 gene for 16S ribosomal RNA, partial sequenceAB9205881287ena1571724
20218Streptomyces griseocarneus 16S rRNA gene, strain DSM40004X999431515ena51201
20218Streptomyces griseocarneus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4095D44004120ena51201
20218Streptomyces griseocarneus gene for 16S rRNA, partial sequence, strain: NBRC 12776AB1841351431ena51201

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces griseocarneus JCM 4580GCA_014655595scaffoldncbi51201
66792Streptomyces griseocarneus CGMCC4.1088GCA_020093395scaffoldncbi51201
66792Streptomyces griseocarneus strain CGMCC4.108851201.10wgspatric51201
66792Streptomyces griseocarneus strain JCM 458051201.8wgspatric51201

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno93.836no
flagellatedno97.827no
gram-positiveyes89.268no
anaerobicno99.153no
aerobicyes89.873no
halophileno92.732no
spore-formingyes95.535no
glucose-utilyes90.656yes
thermophileno97.003yes
glucose-fermentno91.557no

External links

@ref: 9209

culture collection no.: DSM 40004, AS 4.1368, ATCC 12628, ATCC 19763, CBS 501.68, CCM 3228, DSM 41062, ETH 28588, IFO 12776, IFO 3387, ISP 5004, JCM 4095, JCM 4580, KCC S-0095, LMG 5973, NBRC 12776, NBRC 3387, NRRL B-1068, NRRL B-1350, RIA 1043, RIA 132, VKM Ac-881, BCRC 13304, CCUG 11123, CECT 3250, CGMCC 4.1088, CIP 108156, LMG 19383, LMG 8601, MTCC 328, NCIMB 9623, PCM 2326, PCM 2345, CCRC 13304, KCC S-0580, AS 4.1088

straininfo link

  • @ref: 84352
  • straininfo: 265586

literature

  • topic: Phylogeny
  • Pubmed-ID: 35320067
  • title: Streptomyces gobiensis sp. nov., an antimicrobial producing actinobacterium isolated from soil under black Gobi rocks.
  • authors: Wen Y, Zhang G, Bahadur A, Liu Y, Zhang Z, Chen T, Liu G, Zhang W
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.005318
  • year: 2022
  • mesh: *Actinobacteria/genetics, *Anti-Infective Agents, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, Soil Microbiology, *Streptomyces
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9209Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40004)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40004
18462Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40004.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32896Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5747
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69336Wink, J.https://cdn.dsmz.de/wink/DSM%2040004.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84352Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265586.1StrainInfo: A central database for resolving microbial strain identifiers
119745Curators of the CIPCollection of Institut Pasteur (CIP 108156)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108156