Strain identifier
BacDive ID: 15256
Type strain: 
Species: Streptomyces griseoaurantiacus
Strain history: KCC S-0763 <-- IFO 13381 <-- SAJ <-- ISP 5430 <-- N. A. Krassilnikov AK-5.
NCBI tax ID(s): 68213 (species)
version 9.3 (current version)
General
@ref: 9538
BacDive-ID: 15256
DSM-Number: 40430
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive
description: Streptomyces griseoaurantiacus DSM 40430 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from Soil.
NCBI tax id
- NCBI tax id: 68213
- Matching level: species
strain history
| @ref | history |
|---|---|
| 9538 | <- E.B. Shirling, ISP <- N.A. Krassilnikov, INMI |
| 67770 | KCC S-0763 <-- IFO 13381 <-- SAJ <-- ISP 5430 <-- N. A. Krassilnikov AK-5. |
doi: 10.13145/bacdive15256.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces griseoaurantiacus
- full scientific name: Streptomyces griseoaurantiacus (Krassilnikov and Yuan 1965) Pridham 1970 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Actinomyces griseoaurantiacus
@ref: 9538
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces griseoaurantiacus
full scientific name: Streptomyces griseoaurantiacus (Krassilnikov and Yuan 1965) Pridham 1970
type strain: yes
Morphology
cell morphology
| @ref | gram stain | confidence |
|---|---|---|
| 125438 | positive | 90.532 |
| 125439 | positive | 98.9 |
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19453 | Yellow - Brown | 10-14 days | ISP 2 |
| 19453 | Yellow - Brown | 10-14 days | ISP 3 |
| 19453 | Yellow - Brown | 10-14 days | ISP 4 |
| 19453 | Yellow - Brown | 10-14 days | ISP 5 |
| 19453 | Yellow - Brown | 10-14 days | ISP 6 |
| 19453 | Yellow brown | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | complex name | complex color | medium name |
|---|---|---|---|---|
| 19453 | yes | Aerial Mycelium | Grey | ISP 2 |
| 19453 | no | ISP 3 | ||
| 19453 | yes | Aerial Mycelium | Grey | ISP 4 |
| 19453 | yes | Aerial Mycelium | White | ISP 5 |
| 19453 | no | ISP 6 | ||
| 19453 | no | ISP 7 |
multimedia
- @ref: 9538
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40430.jpg
- caption: Medium 65 28°C
- intellectual property rights: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 9538 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf | |
| 9538 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf | |
| 19453 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19453 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19453 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19453 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19453 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19453 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 19453 | positive | optimum | 30 |
| 67770 | positive | growth | 28 |
Physiology and metabolism
oxygen tolerance
- @ref: 125439
- oxygen tolerance: obligate aerobe
- confidence: 98.5
spore formation
| @ref | spore description | type of spore | spore formation | confidence |
|---|---|---|---|---|
| 19453 | Formation of spore chains: rectiflixibilis, spore surface: smooth | spore | yes | |
| 125438 | yes | 90.512 | ||
| 125439 | yes | 91.4 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68368 | 5291 | gelatin | + | hydrolysis |
| 68368 | 27897 | tryptophan | - | energy source |
| 68368 | 16199 | urea | + | hydrolysis |
| 68368 | 16947 | citrate | + | assimilation |
| 68368 | 18257 | ornithine | + | degradation |
| 68368 | 25094 | lysine | + | degradation |
| 68368 | 29016 | arginine | + | hydrolysis |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 68368 | 15688 | acetoin | no |
| 68368 | 35581 | indole | no |
| 68368 | 16136 | hydrogen sulfide | no |
metabolite tests
| @ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
|---|---|---|---|---|
| 68368 | 15688 | acetoin | - | |
| 68368 | 35581 | indole | - |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | gelatinase | + | |
| 68368 | tryptophan deaminase | - | 4.1.99.1 |
| 68368 | urease | + | 3.5.1.5 |
| 68368 | ornithine decarboxylase | + | 4.1.1.17 |
| 68368 | lysine decarboxylase | + | 4.1.1.18 |
| 68368 | arginine dihydrolase | + | 3.5.3.6 |
| 68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 19453 | + | + | + | + | + | - | + | - | - | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 67770
- sample type: Soil
- geographic location: Crimea
- country: USSR
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 9538 | 1 | Risk group (German classification) |
| 19453 | 1 | Risk group (German classification) |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Streptomyces griseoaurantiacus strain DSM 40430 16S ribosomal RNA gene, partial sequence | AY450561 | 1458 | nuccore | 68213 |
| 20218 | Streptomyces griseoaurantiacus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4763 | D44342 | 121 | nuccore | 68213 |
| 20218 | Streptomyces griseoaurantiacus gene for 16S rRNA, partial sequence, strain: NBRC 15440 | AB184676 | 1475 | nuccore | 68213 |
Genome sequences
- @ref: 66792
- description: Streptomyces griseoaurantiacus strain JCM 4763
- accession: 68213.6
- assembly level: wgs
- database: patric
- NCBI tax ID: 68213
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 90.532 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 97.303 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 90.512 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 86.655 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 96 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | no | 88.5 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | yes | 91.4 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | no | 87.6 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | positive | 98.9 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | obligate aerobe | 98.5 |
External links
@ref: 9538
culture collection no.: DSM 40430, ATCC 19840, CBS 682.72, IFO 13381, INMI AK-5, ISP 5430, KCC S-0763, NBRC 13381, RIA 1342, JCM 4763, BCRC 16244, IFO 15440, NBRC 15440, VKM Ac-1728
straininfo link
- @ref: 84351
- straininfo: 389329
literature
- topic: Phylogeny
- Pubmed-ID: 16166691
- title: Streptomyces jietaisiensis sp. nov., isolated from soil in northern China.
- authors: He L, Li W, Huang Y, Wang L, Liu Z, Lanoot B, Vancanneyt M, Swings J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63460-0
- year: 2005
- mesh: Bacterial Typing Techniques, China, Cupressus, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/physiology, Trees
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 9538 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40430) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40430 | |||
| 19453 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40430.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68368 | Automatically annotated from API 20E | |||||
| 84351 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID389329.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |||
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |