Strain identifier

BacDive ID: 15256

Type strain: Yes

Species: Streptomyces griseoaurantiacus

Strain history: KCC S-0763 <-- IFO 13381 <-- SAJ <-- ISP 5430 <-- N. A. Krassilnikov AK-5.

NCBI tax ID(s): 68213 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9538

BacDive-ID: 15256

DSM-Number: 40430

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive

description: Streptomyces griseoaurantiacus DSM 40430 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from Soil.

NCBI tax id

  • NCBI tax id: 68213
  • Matching level: species

strain history

@refhistory
9538<- E.B. Shirling, ISP <- N.A. Krassilnikov, INMI
67770KCC S-0763 <-- IFO 13381 <-- SAJ <-- ISP 5430 <-- N. A. Krassilnikov AK-5.

doi: 10.13145/bacdive15256.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces griseoaurantiacus
  • full scientific name: Streptomyces griseoaurantiacus (Krassilnikov and Yuan 1965) Pridham 1970 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Actinomyces griseoaurantiacus

@ref: 9538

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces griseoaurantiacus

full scientific name: Streptomyces griseoaurantiacus (Krassilnikov and Yuan 1965) Pridham 1970

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

@refcolony colorincubation periodmedium used
19453Yellow - Brown10-14 daysISP 2
19453Yellow - Brown10-14 daysISP 3
19453Yellow - Brown10-14 daysISP 4
19453Yellow - Brown10-14 daysISP 5
19453Yellow - Brown10-14 daysISP 6
19453Yellow brown10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19453yesAerial MyceliumGreyISP 2
19453noISP 3
19453yesAerial MyceliumGreyISP 4
19453yesAerial MyceliumWhiteISP 5
19453noISP 6
19453noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcomposition
19453ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19453ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19453ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19453ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19453ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19453ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19453positiveoptimum30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore descriptiontype of sporespore formationconfidence
19453Formation of spore chains: rectiflixibilis, spore surface: smoothsporeyes
69481yes100
69480yes100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19453+++++-+---+

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Soil
  • geographic location: Crimea
  • country: USSR

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
95381Risk group (German classification)
194531Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces griseoaurantiacus strain DSM 40430 16S ribosomal RNA gene, partial sequenceAY4505611458ena68213
20218Streptomyces griseoaurantiacus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4763D44342121ena68213
20218Streptomyces griseoaurantiacus gene for 16S rRNA, partial sequence, strain: NBRC 15440AB1846761475ena68213

Genome sequences

  • @ref: 66792
  • description: Streptomyces griseoaurantiacus strain JCM 4763
  • accession: 68213.6
  • assembly level: wgs
  • database: patric
  • NCBI tax ID: 68213

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes91.293no
anaerobicno99.142no
halophileno92.656no
spore-formingyes96.007yes
glucose-utilyes89.659no
flagellatedno98.187no
thermophileno97.1yes
motileno93.412no
aerobicyes91.44no
glucose-fermentno87.733no

External links

@ref: 9538

culture collection no.: DSM 40430, ATCC 19840, CBS 682.72, IFO 13381, INMI AK-5, ISP 5430, KCC S-0763, NBRC 13381, RIA 1342, JCM 4763, BCRC 16244, IFO 15440, NBRC 15440, VKM Ac-1728

straininfo link

  • @ref: 84351
  • straininfo: 389329

literature

  • topic: Phylogeny
  • Pubmed-ID: 16166691
  • title: Streptomyces jietaisiensis sp. nov., isolated from soil in northern China.
  • authors: He L, Li W, Huang Y, Wang L, Liu Z, Lanoot B, Vancanneyt M, Swings J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63460-0
  • year: 2005
  • mesh: Bacterial Typing Techniques, China, Cupressus, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/physiology, Trees
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9538Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40430)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40430
19453Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40430.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84351Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389329.1StrainInfo: A central database for resolving microbial strain identifiers