Strain identifier

BacDive ID: 15255

Type strain: Yes

Species: Streptomyces diastaticus

Strain Designation: Duché

Strain history: KCC S-0136 <-- R. Shinobu T-13 <-- IFO 3198 <-- CBS 422.34 <-- J. Duché.

NCBI tax ID(s): 53448 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9462

BacDive-ID: 15255

DSM-Number: 40324

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive

description: Streptomyces diastaticus Duché is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium.

NCBI tax id

  • NCBI tax id: 53448
  • Matching level: species

strain history

@refhistory
9462<- E.B. Shirling, ISP <- CBS
67770KCC S-0136 <-- R. Shinobu T-13 <-- IFO 3198 <-- CBS 422.34 <-- J. Duché.

doi: 10.13145/bacdive15255.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces diastaticus
  • full scientific name: Streptomyces diastaticus (Krainsky 1914) Waksman and Henrici 1948 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Actinomyces diastaticus
    20215Actinomyces rutgersensis
    20215Actinomyces gougeroti
    20215Streptomyces gougerotii
    20215Streptomyces rutgersensis

@ref: 9462

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces gougerotii

full scientific name: Streptomyces diastaticus subsp. diastaticus (Krainsky 1914) Pridham et al. 1958

strain designation: Duché

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

@refcolony colorincubation periodmedium used
19437Yellowish-brown10-14 daysISP 2
19437White10-14 daysISP 3
19437Yellow10-14 daysISP 4
19437Yellow-brown10-14 daysISP 5

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19437yesAerial MyceliumYellow/ReddishISP 2
19437yesAerial MyceliumWhiteISP 3
19437yesAerial MyceliumWhiteISP 4
19437yesAerial MyceliumISP 5

multimedia

  • @ref: 9462
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40324.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9462ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
9462GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19437ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19437ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19437ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19437ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19437positiveoptimum30mesophilic
9462positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore descriptionspore formationconfidence
19437Formation of spore chains: rectiflixibilis, spore surface: smoothyes
69481yes100
69480yes100

compound production

@refcompound
9462gougerotin
20216Gougerotin

halophily

  • @ref: 19437
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1943762968cellulose-
1943716634raffinose-
1943726546rhamnose-
1943728757fructose+
1943729864mannitol+
1943717268myo-inositol-
1943718222xylose-
1943717992sucrose-
1943722599arabinose-
1943717234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19437----+-+--++

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
94621Risk group (German classification)
194371Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218S.gougerotii (DSM 40324T) 16S rRNA geneZ766871476ena53448
20218Streptomyces gougerotii gene for 16S ribosomal RNA, partial sequence, strain: JCM 4478D44173120ena53448
20218Streptomyces gougerotii gene for 16S rRNA, partial sequence, strain: NBRC 13043AB2499821452ena53448
9462Streptomyces gougerotii gene for 16S rRNA, partial sequence, strain: NBRC 3198AB1847421478ena53448

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces gougerotii JCM 4136GCA_014648955contigncbi53448
66792Streptomyces gougerotii strain JCM 413653448.4wgspatric53448
66792Streptomyces gougerotii strain NBRC 1304353448.3wgspatric53448
67770Streptomyces gougerotii NBRC 13043GCA_011170145contigncbi53448

GC content

@refGC-contentmethod
6777071fluorimetric
6777073.6genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes91.989no
anaerobicno99.408no
halophileno91.772no
spore-formingyes94.478no
glucose-utilyes89.193no
thermophileno97.623yes
aerobicyes94.304no
motileno96.013no
glucose-fermentno88.792no
flagellatedno97.787no

External links

@ref: 9462

culture collection no.: DSM 40324, AS 4.1854, ATCC 10975, ATCC 25455, CBS 422.34, CBS 688.69, ETH 10213, IFO 13043, IFO 3198, IMRU 3590, ISP 5324, JCM 4136, JCM 4478, KCC S-0136, KCC S-0478, NBRC 13043, NBRC 3198, PSA 224, BCRC 12105, CGMCC 4.1854, CGMCC 4.1885, CGMCC 4.2081, IMET 40289, NRRL B-1344, NRRL B-1903, RIA 1235, VKM Ac-713

literature

  • topic: Phylogeny
  • Pubmed-ID: 24002610
  • title: Streptomyces polyrhachii sp. nov., a novel actinomycete isolated from an edible Chinese black ant (Polyrhachis vicina Roger).
  • authors: Yu C, Liu C, Wang X, Zhao J, Yang L, Gao R, Zhang Y, Xiang W
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-013-0021-3
  • year: 2013
  • mesh: Animals, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Hymenoptera/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/*isolation & purification/metabolism
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9462Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40324)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40324
19437Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40324.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1