Strain identifier
BacDive ID: 15249
Type strain:
Species: Streptomyces globosus
Strain Designation: BJ-333
Strain history: DSM 40815 <-- H. D. Tresner BJ-333.
NCBI tax ID(s): 68209 (species)
General
@ref: 9819
BacDive-ID: 15249
DSM-Number: 40815
keywords: 16S sequence, Bacteria, spore-forming, mesophilic
description: Streptomyces globosus BJ-333 is a spore-forming, mesophilic bacterium that builds an aerial mycelium.
NCBI tax id
- NCBI tax id: 68209
- Matching level: species
strain history
@ref | history |
---|---|
9819 | <- H.D. Tresner, BJ-333 |
67770 | DSM 40815 <-- H. D. Tresner BJ-333. |
doi: 10.13145/bacdive15249.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces globosus
- full scientific name: Streptomyces globosus (Krassilnikov 1941) Waksman 1953 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Actinomyces globosus
@ref: 9819
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces globosus
full scientific name: Streptomyces globosus (Krassilnikov 1941) Waksman 1953
strain designation: BJ-333
type strain: yes
Morphology
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
20214 | Brown | 10-14 days | ISP 2 |
20214 | Yellow | 10-14 days | ISP 3 |
20214 | Yellow | 10-14 days | ISP 4 |
20214 | Yellow | 10-14 days | ISP 5 |
20214 | Yellow | 10-14 days | ISP 6 |
20214 | Brown | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
20214 | yes | Aerial Mycelium | Grey/brown | ISP 2 |
20214 | yes | Aerial Mycelium | Beige | ISP 3 |
20214 | yes | Aerial Mycelium | Beige | ISP 4 |
20214 | yes | Aerial Mycelium | Beige | ISP 5 |
20214 | no | ISP 6 | ||
20214 | yes | Aerial Mycelium | Beige | ISP 7 |
multimedia
- @ref: 9819
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40815.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
9819 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
20214 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
20214 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
20214 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
20214 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
20214 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
20214 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
9819 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
20214 | positive | optimum | 28 | mesophilic |
9819 | positive | growth | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
- @ref: 20214
- spore description: Formation of spore chains (rectiflixibilis), spore surface smooth
- spore formation: yes
halophily
- @ref: 20214
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
20214 | 17234 | glucose | + | |
20214 | 22599 | arabinose | - | |
20214 | 17992 | sucrose | - | |
20214 | 18222 | xylose | +/- | |
20214 | 17268 | myo-inositol | + | |
20214 | 29864 | mannitol | - | |
20214 | 28757 | fructose | + | |
20214 | 26546 | rhamnose | - | |
20214 | 16634 | raffinose | + | |
20214 | 62968 | cellulose | - | |
68368 | 5291 | gelatin | - | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | - | hydrolysis |
68368 | 16947 | citrate | - | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | yes |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | yes |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | + | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | gelatinase | - | |
68368 | tryptophan deaminase | + | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | + | 3.5.3.6 |
68368 | beta-galactosidase | - | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
20214 | - | + | - | - | - | + | - | + | - | + | - |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
9819 | 1 | Risk group (German classification) |
20214 | 1 | German classification |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptomyces sp. NTG3 gene for 16S ribosomal RNA, partial sequence | AB920574 | 1281 | ena | 1571710 |
20218 | Streptomyces globosus 16S rRNA gene, type strain LMG 19896 | AJ781330 | 1478 | ena | 68209 |
External links
@ref: 9819
culture collection no.: DSM 40815, ATCC 14979, IMRU 3736, JCM 13859, NRRL B-2292, CGMCC 4.1969, LMG 19896, NCIMB 12861
straininfo link
- @ref: 84346
- straininfo: 36221
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 24664662 | Streptomyces amritsarensis sp. nov., exhibiting broad-spectrum antimicrobial activity. | Sharma D, Mayilraj S, Manhas RK | Antonie Van Leeuwenhoek | 10.1007/s10482-014-0151-2 | 2014 | Anti-Infective Agents/*metabolism, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fungi/drug effects, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects, India, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/metabolism | Pathogenicity |
Phylogeny | 26703868 | Streptomyces yangpuensis sp. nov., isolated from soil. | Tang B, Yu Y, Zhi X, Yang L, Cen X, Zhao G, Ding X | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000861 | 2015 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
9819 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40815) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40815 | |||
20214 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40815.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
84346 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID36221.1 | StrainInfo: A central database for resolving microbial strain identifiers |