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Strain identifier

BacDive ID: 15249

Type strain: Yes

Species: Streptomyces globosus

Strain Designation: BJ-333

Strain history: DSM 40815 <-- H. D. Tresner BJ-333.

NCBI tax ID(s): 68209 (species)

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@ref: 9819

BacDive-ID: 15249

DSM-Number: 40815

keywords: 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces globosus BJ-333 is a spore-forming, mesophilic bacterium that builds an aerial mycelium.

NCBI tax id

  • NCBI tax id: 68209
  • Matching level: species

strain history

doi: 10.13145/bacdive15249.20221219.7.1

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces globosus
  • full scientific name: Streptomyces globosus (Krassilnikov 1941) Waksman 1953 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Actinomyces globosus

@ref: 9819

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces globosus

full scientific name: Streptomyces globosus (Krassilnikov 1941) Waksman 1953

strain designation: BJ-333

type strain: yes


cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 96.114

colony morphology

@refcolony colorincubation periodmedium used
20214Brown10-14 daysISP 2
20214Yellow10-14 daysISP 3
20214Yellow10-14 daysISP 4
20214Yellow10-14 daysISP 5
20214Yellow10-14 daysISP 6
20214Brown10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
20214yesAerial MyceliumGrey/brownISP 2
20214yesAerial MyceliumBeigeISP 3
20214yesAerial MyceliumBeigeISP 4
20214yesAerial MyceliumBeigeISP 5
20214noISP 6
20214yesAerial MyceliumBeigeISP 7


  • @ref: 9819
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40815.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

9819GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20214ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20214ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20214ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20214ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20214ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20214ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9819ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp


Physiology and metabolism

spore formation

@refspore descriptionspore formationconfidence
20214Formation of spore chains (rectiflixibilis), spore surface smoothyes


@refsaltgrowthtested relationconcentrationhalophily levelconfidence
20214NaClpositivemaximum5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836827897tryptophan-energy source

metabolite production

6836816136hydrogen sulfideyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test


68368tryptophan deaminase+
68368ornithine decarboxylase-
68368lysine decarboxylase-
68368arginine dihydrolase+



Safety information

risk assessment

@refbiosafety levelbiosafety level comment
98191Risk group (German classification)
202141German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces sp. NTG3 gene for 16S ribosomal RNA, partial sequenceAB9205741281ena1571710
20218Streptomyces globosus 16S rRNA gene, type strain LMG 19896AJ7813301478ena68209

External links

@ref: 9819

culture collection no.: DSM 40815, ATCC 14979, IMRU 3736, JCM 13859, NRRL B-2292, CGMCC 4.1969, LMG 19896, NCIMB 12861

straininfo link



Phylogeny24664662Streptomyces amritsarensis sp. nov., exhibiting broad-spectrum antimicrobial activity.Sharma D, Mayilraj S, Manhas RKAntonie Van Leeuwenhoek10.1007/s10482-014-0151-22014Anti-Infective Agents/*metabolism, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fungi/drug effects, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects, India, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/metabolismPathogenicity
Phylogeny26703868Streptomyces yangpuensis sp. nov., isolated from soil.Tang B, Yu Y, Zhi X, Yang L, Cen X, Zhao G, Ding XInt J Syst Evol Microbiol10.1099/ijsem.0.0008612015


9819Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40815)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40815
20214Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40815.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)