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Strain identifier

BacDive ID: 15235

Type strain: Yes

Species: Streptomyces globisporus subsp. globisporus

Strain history: KCC S-0378 <-- IFO 12867 <-- SAJ <-- ISP 5199 <-- INMI 2302.

NCBI tax ID(s): 1908 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9373

BacDive-ID: 15235

DSM-Number: 40199

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Streptomyces globisporus subsp. globisporus DSM 40199 is a mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1908
  • Matching level: species

strain history

doi: 10.13145/bacdive15235.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces globisporus subsp. globisporus
  • full scientific name: Streptomyces globisporus subsp. globisporus (Krassilnikov 1941) Pridham et al. 1958 (Approved Lists 1980)

@ref: 9373

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces globisporus subsp. globisporus

full scientific name: Streptomyces globisporus subsp. globisporus (Krassilnikov 1941) Pridham et al. 1958

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 94.811

colony morphology

@refcolony colorincubation periodmedium used
19423Ochre yellow10-14 daysISP 2
19423Ivory10-14 daysISP 3
19423Green brown10-14 daysISP 4
19423Ivory10-14 daysISP 5
19423Sand yellow10-14 daysISP 6
19423Beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19423yesAerial MyceliumOyster whiteISP 2
19423yesAerial MyceliumOyster whiteISP 3
19423yesAerial MyceliumLight greyISP 4
19423noISP 5
19423noISP 6
19423noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9373GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19423ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19423ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19423ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19423ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19423ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19423ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19423positiveoptimum28mesophilic
9373positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 97.654

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 98.983

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1942362968cellulose+
1942316634raffinose-
1942326546rhamnose+
1942328757fructose+
1942329864mannitol+
1942317268myo-inositol-
1942318222xylose-
1942317992sucrose-
1942322599arabinose+
1942317234glucose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase+3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19423+-+++++++++-+-+++-+

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19423+++++-+--++

Isolation, sampling and environmental information

isolation

  • @ref: 9373
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_135.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_120;99_135&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: HQ995504
  • Sequence Identity:
  • Total samples: 4030
  • soil counts: 2441
  • aquatic counts: 286
  • animal counts: 1009
  • plant counts: 294

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
93731Risk group (German classification)
194231Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces globisporus subsp. globisporus gene for 16S rRNA, partial sequence, strain: NBRC 12867AB1842031472ena66858
9373Streptomyces globisporus subsp. globisporus strain NRRL B-2872 16S ribosomal RNA gene, partial sequenceEF1786861505ena66858
9373Streptomyces globisporus strain KCTC 9026 16S ribosomal RNA gene, partial sequenceHQ9955041403ena1908

Genome sequences

  • @ref: 66792
  • description: Streptomyces globisporus strain JCM 4378
  • accession: 1908.13
  • assembly level: wgs
  • database: patric
  • NCBI tax ID: 1908

GC content

  • @ref: 67770
  • GC-content: 73
  • method: fluorimetric

External links

@ref: 9373

culture collection no.: DSM 40199, ATCC 15864, ATCC 23913, CBS 834.68, IFO 12867, INMI 2302, ISP 5199, KCTC 9026, NBRC 12867, NRRL B-2872, RIA 1151, RIA 335, JCM 4378, BCRC 11479, CCUG 11107, NCAIM B.01476, NCIMB 9796, VKM Ac-179

straininfo link

@refpassport
20218http://www.straininfo.net/strains/51392
20218http://www.straininfo.net/strains/51387
20218http://www.straininfo.net/strains/51388
20218http://www.straininfo.net/strains/230346
20218http://www.straininfo.net/strains/51393
20218http://www.straininfo.net/strains/51394
20218http://www.straininfo.net/strains/51395
20218http://www.straininfo.net/strains/319260
20218http://www.straininfo.net/strains/334417
20218http://www.straininfo.net/strains/51399
20218http://www.straininfo.net/strains/51400
20218http://www.straininfo.net/strains/51401

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9373Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40199)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40199
19423Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40199.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)