Strain identifier

BacDive ID: 15209

Type strain: Yes

Species: Streptomyces fumigatiscleroticus

Strain Designation: 145

Strain history: KCC A-0101 <-- RIA 884 <-- HACC 145.

NCBI tax ID(s): 66371 (species)

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General

@ref: 10823

BacDive-ID: 15209

DSM-Number: 43154

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-negative, rod-shaped

description: Streptomyces fumigatiscleroticus 145 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil in Hwaseong, South Korea.

NCBI tax id

  • NCBI tax id: 66371
  • Matching level: species

strain history

@refhistory
10823<- KCC <- V.D. Kuznetsov, RIA <- Hindustan Antibiotics Ltd., 145
67770KCC A-0101 <-- RIA 884 <-- HACC 145.

doi: 10.13145/bacdive15209.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces fumigatiscleroticus
  • full scientific name: Streptomyces fumigatiscleroticus (ex Pridham 1970) Goodfellow et al. 1986

  • synonyms

    @refsynonym
    20215Streptomyces fumigatiscleroticus
    20215Chainia fumigata

@ref: 10823

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces fumigatiscleroticus

full scientific name: Streptomyces fumigatiscleroticus (ex Pridham 1970) Goodfellow et al. 1986

strain designation: 145

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43974negative1.0-1.4 µm0.6-0.9 µmrod-shapedno
69480positive100

colony morphology

@refcolony colorincubation periodmedium usedcolony sizecolony shape
19941Yellow10-14 daysISP 2
19941Yellow/brown10-14 daysISP 3
19941Colorless10-14 daysISP 4
19941Red brown10-14 daysISP 5
19941Colorless10-14 daysISP 6
19941Red brown10-14 daysISP 7
43974light pink4 daysR2A agar1.0-2.0 mmcircular

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
19941yesAerial MyceliumISP 2
19941yesAerial MyceliumISP 3
19941yesAerial MyceliumISP 4White
19941yesAerial MyceliumISP 5White
19941noISP 6
19941yesAerial MyceliumISP 7Gray

pigmentation

  • @ref: 43974
  • production: no
  • name: Flexirubin-type pigments

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10823GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19941ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19941ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19941ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19941ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19941ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19941ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
43974LB (Luria-Bertani) MEDIUMyes
43974MacConkey agarno
43974Marine agar (MA)yes
43974Nutrient agar (NA)yes
43974Reasoner's 2A agar (R2A)yes
43974Tryptone soya agaryes
10823ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19941positiveoptimum28mesophilic
10823positivegrowth28mesophilic
43974positivegrowth10-42
43974positiveoptimum25-32mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43974positivegrowth5.0-10.5alkaliphile
43974positiveoptimum6.5-8.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43974
  • oxygen tolerance: aerobe

spore formation

@refspore descriptionspore formationconfidence
19941Formation of spore chains (spirales)(Sp), spore surface smoothyes
43974no
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
43974NaClpositiveoptimum0 %(w/v)
43974NaClpositivegrowth0-6 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-9(H8), MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1994162968cellulose-
1994116634raffinose+
1994126546rhamnose+
1994128757fructose+
1994129864mannitol+
1994117268myo-inositol-
1994118222xylose-
1994117992sucrose-
1994122599arabinose+
1994117234glucose-
43974casein-assimilation
4397428017starch-assimilation
43974168082-dehydro-D-gluconate-builds acid from
43974370543-hydroxybutyrate-builds acid from
43974581435-dehydro-D-gluconate-builds acid from
4397427689decanoate-builds acid from
4397417634D-glucose-builds acid from
4397416899D-mannitol-builds acid from
4397416988D-ribose-builds acid from
4397417924D-sorbitol-builds acid from
4397428087glycogen-builds acid from
4397417268myo-inositol-builds acid from
4397417240itaconate-builds acid from
4397416977L-alanine-builds acid from
4397416467L-arginine-builds acid from
4397418287L-fucose-builds acid from
4397415971L-histidine-builds acid from
4397417203L-proline-builds acid from
4397417115L-serine-builds acid from
4397416828L-tryptophan-builds acid from
4397418401phenylacetate-builds acid from
4397453258sodium citrate-builds acid from
4397462983sodium malonate-builds acid from
439749300suberic acid-builds acid from
4397416199urea-builds acid from
4397417234glucose-fermentation
4397417234glucose+assimilation
439743557154-nitrophenyl beta-D-galactopyranoside+builds acid from
4397417634D-glucose+builds acid from
4397433801D-saccharate+builds acid from
43974esculin ferric citrate+builds acid from
439745291gelatin+builds acid from
4397430849L-arabinose+builds acid from
4397462345L-rhamnose+builds acid from
4397425115malate+builds acid from
4397417306maltose+builds acid from
4397437684mannose+builds acid from
4397428053melibiose+builds acid from
43974506227N-acetylglucosamine+builds acid from
4397417272propionate+builds acid from
4397417814salicin+builds acid from
4397432954sodium acetate+builds acid from
4397431011valerate+builds acid from
4397453426tween 80+hydrolysis
4397417632nitrate+reduction
43974161933-hydroxybenzoate+/-builds acid from
43974178794-hydroxybenzoate+/-builds acid from
4397417128adipate+/-builds acid from
4397424996lactate+/-builds acid from
4397432032potassium gluconate+/-builds acid from
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4397427902tetracyclineyesyes
4397417833gentamicinyesyes10 µg (disc)
4397417698chloramphenicolyesyes100 µg (disc)
439746104kanamycinyesyes30 µg (disc)
4397417076streptomycinyesyes
439747507neomycinyesyes30 µg (disc)
4397428077rifampicinyesyes30 µg (disc)
4397445924trimethoprimyesyes30 µg (disc)
4397428368novobiocinyesyes30 µg (disc)
43974100147nalidixic acidyesyes30 µg (disc)
4397427641cycloheximideyesyes
4397428971ampicillinyesyes
439749332sulfamethoxazoleyesyes30 µg (disc)
4397417334penicillinyesyes

metabolite production

@refChebi-IDmetaboliteproduction
4397416136hydrogen sulfideno
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
43974catalase+1.11.1.6
43974cytochrome oxidase-1.9.3.1
43974DNase-
43974alkaline phosphatase+3.1.3.1
43974leucine arylamidase+3.4.11.1
43974valine arylamidase+
43974cystine arylamidase+3.4.11.3
43974trypsin+3.4.21.4
43974acid phosphatase+3.1.3.2
43974naphthol-AS-BI-phosphohydrolase+
43974alpha-galactosidase+3.2.1.22
43974beta-galactosidase+3.2.1.23
43974alpha-glucosidase+3.2.1.20
43974beta-glucosidase+3.2.1.21
43974N-acetyl-beta-glucosaminidase+3.2.1.52
43974alpha-chymotrypsin+/-3.4.21.1
43974alpha-mannosidase+/-3.2.1.24
43974esterase (C 4)-
43974esterase Lipase (C 8)-
43974lipase (C 14)-
43974beta-glucuronidase-3.2.1.31
43974alpha-fucosidase-3.2.1.51
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43974C11:0 anteiso1.1
    43974C15:0 anteiso1.9
    43974C14:02.7
    43974C15:1ω6c1.2
    43974C16:06
    43974C16:0 10-methyl / iso-C17:1ω9c2.4
    43974C16:0 3OH2.1
    43974C16:1ω5c2.7
    43974C16:1ω7c / C16:1ω6c37
    4397410-methyl C18:02.2
    43974C18:1ω9c2.1
    43974C15:0 iso19.2
    43974C15:0 iso 3OH2
    43974C16:0 iso 3OH1.6
    43974C17:0 iso 3OH8.1
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A agar
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 2
  • software version: Sherlock 6.0B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890 gas chromatograph
  • method/protocol: Sasser, 1990
  • cutoff value:

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19941-++++-+--++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperature
43974soil in Hwaseong, South KoreaHwaseongRepublic of KoreaKORAsia37.2694126.762R2A agar2 weeks28
67770SoilPimpriIndiaINDAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
108231Risk group (German classification)
199411German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces fumigatiscleroticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 3101D43979121ena66371
20218Streptomyces fumigatiscleroticus gene for 16S rRNA, partial sequence, strain: NBRC 12999AB1842481474ena66371
20218Streptomyces fumigatiscleroticus strain NRRL B-3856T 16S ribosomal RNA gene, partial sequenceDQ4424991492ena66371

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces fumigatiscleroticus JCM 3101GCA_014647975scaffoldncbi66371
66792Streptomyces fumigatiscleroticus strain JCM 310166371.3wgspatric66371

GC content

  • @ref: 43974
  • GC-content: 40.4
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
spore-formingyes100no
flagellatedno98.24yes
gram-positiveyes87.739yes
anaerobicno99.247no
aerobicyes94.925yes
halophileno94.113no
spore-formingyes96.062no
thermophileno98.146yes
glucose-utilyes89.921no
motileno95.292yes
glucose-fermentno89.741yes

External links

@ref: 10823

culture collection no.: DSM 43154, ATCC 19345, JCM 3101, KCC A-0101, RIA 884, BCRC 12344, CBS 639.66, CGMCC 4.1909, CMI 117720, IFO 12999, NBRC 12999, NCIMB 11004, NRRL B-3856

straininfo link

  • @ref: 84307
  • straininfo: 389374

literature

  • topic: Phylogeny
  • Pubmed-ID: 35446756
  • title: Streptomyces sennicomposti sp. nov., an actinomycete isolated from compost of Senna siamea (Lam.).
  • authors: Duangupama T, Pittayakhajonwut P, Intaraudom C, Suriyachadkun C, Sirirote P, He YW, Thawai C
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.005320
  • year: 2022
  • mesh: *Actinobacteria/genetics, Bacterial Typing Techniques, Base Composition, *Composting, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces, Thailand
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10823Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43154)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43154
19941Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43154.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
43974Ram Hari Dahal, Jaisoo Kim10.1099/ijsem.0.001010Pedobacter humicola sp. nov., a member of the genus Pedobacter isolated from soilIJSEM 66: 2205-2211 201626956360
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84307Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389374.1StrainInfo: A central database for resolving microbial strain identifiers