Strain identifier

BacDive ID: 15206

Type strain: Yes

Species: Streptomyces microflavus

Strain Designation: A26

Strain history: CIP <- 1998, D.P. Labeda, Nat. Center for Agri. Utilization Res., Peoria, Illinois, USA <- IMRU <- R.A. Benedict: strain A26

NCBI tax ID(s): 1303692 (strain), 1919 (species)

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General

@ref: 9669

BacDive-ID: 15206

DSM-Number: 40593

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive

description: Streptomyces microflavus A26 is an obligate aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium.

NCBI tax id

NCBI tax idMatching level
1303692strain
1919species

strain history

@refhistory
9669<- E.B. Shirling, ISP <- NRRL
67770KCC S-0129 <-- M. Mayama <-- NRRL B-1453 <-- S. A. Waksman.
119486CIP <- 1998, D.P. Labeda, Nat. Center for Agri. Utilization Res., Peoria, Illinois, USA <- IMRU <- R.A. Benedict: strain A26

doi: 10.13145/bacdive15206.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces microflavus
  • full scientific name: Streptomyces microflavus (Krainsky 1914) Waksman and Henrici 1948 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Actinomyces alboviridis
    20215Actinomyces fulvissimus
    20215Actinomyces willmorei
    20215Actinomyces lipmanii
    20215Actinomyces griseus subsp. alpha
    20215Streptomyces fulvissimus
    20215Streptomyces griseus subsp. alpha
    20215Streptomyces griseus subsp. cretosus
    20215Actinomyces microflavus
    20215Streptomyces lipmanii
    20215Streptomyces alboviridis
    20215Streptomyces willmorei
    20215Streptomyces luridiscabiei

@ref: 9669

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Kitasatosporales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces microflavus

full scientific name: Streptomyces microflavus (Krainsky 1914) Waksman and Henrici 1948 emend. Lanoot et al. 2005

strain designation: A26

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
119486positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
19481Orange10-14 daysISP 2
19481Red10-14 daysISP 3
19481Red10-14 daysISP 4
19481Red10-14 daysISP 5
119486

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19481yesAerial MyceliumOrangeISP 2
19481yesAerial MyceliumRedISP 3
19481yesAerial MyceliumRedISP 4
19481yesAerial MyceliumWhite/ orangeISP 5
19481noISP 6
19481noISP 7

multimedia

@refmultimedia contentintellectual property rightscaption
9669https://www.dsmz.de/microorganisms/photos/DSM_40593-1.jpg© Leibniz-Institut DSMZ
9669https://www.dsmz.de/microorganisms/photos/DSM_40593-2.jpg© Leibniz-Institut DSMZ
9669https://www.dsmz.de/microorganisms/photos/DSM_40593-3.jpg© Leibniz-Institut DSMZ
9669https://www.dsmz.de/microorganisms/photos/DSM_40593-4.jpg© Leibniz-Institut DSMZ
9669https://www.dsmz.de/microorganisms/photos/DSM_40593.jpg© Leibniz-Institut DSMZMedium 65 28°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9669GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19481ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19481ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19481ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19481ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
39717MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
9669ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
119486CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57
119486CIP Medium 236yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=236

culture temp

@refgrowthtypetemperaturerange
19481positiveoptimum30mesophilic
9669positivegrowth28mesophilic
39717positivegrowth30mesophilic
67770positivegrowth28mesophilic
119486positivegrowth25-37mesophilic
119486nogrowth10psychrophilic
119486nogrowth41thermophilic
119486nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119486
  • oxygen tolerance: obligate aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
19481Formation of spore chains (rectiflixibilis), spore surface smoothsporeyes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
19481NaClpositivemaximum2.5 %
119486NaClpositivegrowth0-4 %
119486NaClnogrowth6 %
119486NaClnogrowth8 %
119486NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1948162968cellulose-
1948116634raffinose-
1948126546rhamnose-
1948128757fructose-
1948129864mannitol+/-
1948117268myo-inositol-
1948118222xylose-
1948117992sucrose-
1948122599arabinose-
1948117234glucose+
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
11948616947citrate-carbon source
1194864853esculin+hydrolysis
119486606565hippurate+hydrolysis
11948617632nitrate-reduction
11948616301nitrite-reduction
11948617632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11948635581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
11948615688acetoin-
11948617234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
119486oxidase-
119486beta-galactosidase+3.2.1.23
119486alcohol dehydrogenase-1.1.1.1
119486gelatinase-
119486amylase+
119486caseinase+3.4.21.50
119486catalase+1.11.1.6
119486tween esterase+
119486gamma-glutamyltransferase+2.3.2.2
119486lecithinase+
119486lipase+
119486lysine decarboxylase-4.1.1.18
119486ornithine decarboxylase-4.1.1.17
119486phenylalanine ammonia-lyase-4.3.1.24
119486protease-
119486tryptophan deaminase-
119486urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119486-+++-+-+++++-----+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19481+---+-++--+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119486+/----+/---+/--+/-+++/---------+--+/-------+/----+/-+/---+------+/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119486+++++-+-++-----+--++----+-+----+++---+-----------+---------++-----------+-----+++---+---+-++++-----

Isolation, sampling and environmental information

isolation

  • @ref: 119486
  • isolation date: 1952

taxonmaps

  • @ref: 69479
  • File name: preview.99_4034.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_91;97_287;98_3057;99_4034&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: LM999765
  • Sequence Identity:
  • Total samples: 91
  • soil counts: 70
  • aquatic counts: 4
  • animal counts: 16
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
96691Risk group (German classification)
194811Risk group (German classification)
1194861Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces fulvissimus gene for 16S rRNA, partial sequence, strain: NBRC 13482AB1844341475ena68202
20218Streptomyces fulvissimus strain NRRL B-1453 16S ribosomal RNA gene, partial sequenceAY9999181325ena68202
9669Streptomyces fulvissimus partial 16S rRNA gene, type strain DSM 40593TLM9997651502ena68202
67770Streptomyces fulvissimus gene for 16S rRNA, partial sequence, strain: NBRC 3717AB1847871476ena68202

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces microflavus DSM 40593GCA_000385945completencbi1303692
66792Streptomyces fulvissimus DSM 405931303692.3completepatric1303692
66792Streptomyces fulvissimus DSM 405932554235391completeimg68202

GC content

  • @ref: 67770
  • GC-content: 71.5
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno94.189no
flagellatedno97.96no
gram-positiveyes90.336no
anaerobicno99.031no
aerobicyes92.493no
halophileno94.077no
spore-formingyes94.269no
thermophileno99.395yes
glucose-utilyes88.567yes
glucose-fermentno85.731no

External links

@ref: 9669

culture collection no.: DSM 40593, ATCC 27431, CBS 783.72, IFO 13482, ISP 5593, NBRC 13482, NCIB 9609, NRRL B-1453, RIA 1443, JCM 4129, BCRC 12172, CCUG 11114, CGMCC 4.1302, CGMCC 4.1524, CIP 105783, IFO 3717, JCM 4754, KCTC 9779, LMG 19310, NBRC 3717, NCIMB 10505, NCIMB 9609, VKM Ac-994, IMRU 3665

straininfo link

  • @ref: 84304
  • straininfo: 14103

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16458865Cell wall teichoic acids of streptomycetes of the phenetic cluster 'Streptomyces fulvissimus'.Shashkov AS, Streshinskaya GM, Senchenkova SN, Kozlova YI, Alferova IV, Terekhova LP, Evtushenko LICarbohydr Res10.1016/j.carres.2006.01.0202006Carbohydrate Conformation, Cell Wall/*chemistry, Nuclear Magnetic Resonance, Biomolecular, Streptomyces/*chemistry/classification/*cytology, Teichoic Acids/*chemistry
Phylogeny20525816Streptomyces tacrolimicus sp. nov., a low producer of the immunosuppressant tacrolimus (FK506).Martinez-Castro M, Barreiro C, Romero F, Fernandez-Chimeno RI, Martin JFInt J Syst Evol Microbiol10.1099/ijs.0.024273-02010DNA, Bacterial/genetics, Immunosuppressive Agents/*metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Streptomyces/*classification/genetics/*isolation & purification/metabolism, Tacrolimus/*metabolismMetabolism
Genetics23965270Complete genome sequence of Streptomyces fulvissimus.Myronovskyi M, Tokovenko B, Manderscheid N, Petzke L, Luzhetskyy AJ Biotechnol10.1016/j.jbiotec.2013.08.0132013Genome, Bacterial/*genetics, Molecular Sequence Data, Streptomyces/*genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9669Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40593)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40593
19481Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40593.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39717Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17840
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84304Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14103.1StrainInfo: A central database for resolving microbial strain identifiers
119486Curators of the CIPCollection of Institut Pasteur (CIP 105783)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105783