Strain identifier
BacDive ID: 15154
Type strain:
Species: Streptomyces flaveolus
Strain Designation: Actinomyces 168
Variant: Isotype of BacDive ID 15155
Strain history: KCC S-0032 <-- T. Yamaguchi (IAM 0005) <-- MTHU 65 <-- Hyden Chem. Corp. <-- ATCC 3319 <-- IMRU 3319.
NCBI tax ID(s): 67297 (species)
General
@ref: 9243
BacDive-ID: 15154
DSM-Number: 40061
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, amino acid production
description: Streptomyces flaveolus Actinomyces 168 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces amino acids.
NCBI tax id
- NCBI tax id: 67297
- Matching level: species
strain history
@ref | history |
---|---|
9243 | <- E.B. Shirling, ISP <- S.A. Waksman, IMRU |
67770 | KCC S-0032 <-- T. Yamaguchi (IAM 0005) <-- MTHU 65 <-- Hyden Chem. Corp. <-- ATCC 3319 <-- IMRU 3319. |
doi: 10.13145/bacdive15154.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces flaveolus
- full scientific name: Streptomyces flaveolus (Waksman 1923) Waksman and Henrici 1948 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Actinomyces flaveolus
@ref: 9243
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces flaveolus
full scientific name: Streptomyces flaveolus (Waksman 1923) Waksman and Henrici 1948
strain designation: Actinomyces 168
variant: Isotype of BacDive ID 15155
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 90.327 | |
69480 | 100 | positive |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19396 | Yellow | 10-14 days | ISP 2 |
19396 | Yellow | 10-14 days | ISP 3 |
19396 | Yellow | 10-14 days | ISP 4 |
19396 | Yellow | 10-14 days | ISP 5 |
19396 | Yellow | 10-14 days | ISP 6 |
19396 | Yellow | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
19396 | yes | Aerial Mycelium | Grey | ISP 2 |
19396 | yes | Aerial Mycelium | Grey | ISP 3 |
19396 | yes | Aerial Mycelium | Grey | ISP 4 |
19396 | yes | Aerial Mycelium | Grey | ISP 5 |
19396 | no | ISP 6 | ||
19396 | yes | Aerial Mycelium | White | ISP 7 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
9243 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
19396 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19396 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19396 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19396 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19396 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19396 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
9243 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19396 | positive | optimum | 28 | mesophilic |
9243 | positive | growth | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
@ref | spore description | spore formation | confidence |
---|---|---|---|
19396 | Formation of spore chains: rectiflixibilis, spore surface: hairy | yes | |
69481 | yes | 100 | |
69480 | yes | 100 |
compound production
- @ref: 9243
- compound: L isoleucine
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
19396 | 62968 | cellulose | + | |
19396 | 16634 | raffinose | + | |
19396 | 26546 | rhamnose | + | |
19396 | 28757 | fructose | + | |
19396 | 29864 | mannitol | + | |
19396 | 17268 | myo-inositol | + | |
19396 | 18222 | xylose | + | |
19396 | 17992 | sucrose | + | |
19396 | 22599 | arabinose | + | |
19396 | 17234 | glucose | + | |
68368 | 5291 | gelatin | + | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | + | hydrolysis |
68368 | 16947 | citrate | - | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | - | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | gelatinase | + | |
68368 | tryptophan deaminase | + | 4.1.99.1 |
68368 | urease | + | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
19396 | + | - | - | - | - | - | + | + | - | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 9243
- sample type: soil
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
9243 | 1 | Risk group (German classification) |
19396 | 1 | Risk group (German classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptomyces flaveolus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4577 | D44245 | 121 | ena | 67297 |
20218 | Streptomyces flaveolus gene for 16S rRNA, partial sequence, strain: NBRC 12768 | AB184131 | 1463 | ena | 67297 |
20218 | Streptomyces flaveolus gene for 16S rRNA, partial sequence, strain: NBRC 3408 | AB184764 | 1477 | ena | 67297 |
20218 | Streptomyces flaveolus gene for 16S rRNA, partial sequence, strain: NBRC 3715 | AB184786 | 1477 | ena | 67297 |
20218 | Streptomyces flaveolus strain NRRL B-1334 16S ribosomal RNA gene, partial sequence | EF654098 | 1481 | ena | 67297 |
20218 | Streptomyces flaveolus strain NRRL B-2688 16S ribosomal RNA gene, partial sequence | AY999799 | 1192 | ena | 67297 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Streptomyces flaveolus JCM 4032 | GCA_014648815 | contig | ncbi | 67297 |
66792 | Streptomyces flaveolus strain JCM 4032 | 67297.6 | wgs | patric | 67297 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
gram-positive | yes | 89.882 | no |
anaerobic | no | 99.311 | no |
halophile | no | 93.557 | no |
spore-forming | yes | 96.308 | no |
glucose-util | yes | 91.393 | yes |
thermophile | no | 98.494 | no |
flagellated | no | 98.196 | no |
aerobic | yes | 94.548 | no |
glucose-ferment | no | 90.595 | no |
motile | no | 94.547 | no |
External links
@ref: 9243
culture collection no.: DSM 40061, ATCC 19754, ATCC 25460, ATCC 3319, CBS 493.68, CBS 693.69, DSM 40328, ETH 12399, IAM 0117, IFO 12768, IMET 40233, IMRU 3319, ISP 5061, ISP 5328, JCM 4032, JCM 4577, KCC S-0032, KCC S-0577, NBRC 12768, NRRL B-1334, NRRL B-2688, RIA 1035, BCRC 12489, CBS 128.20, CCM 3171, CECT 3181, CGMCC 4.1432, HAMBI 893, ICMP 392, IFO 3408, IFO 3715, KCTC 9022, LMG 19328, NBRC 3408, NBRC 3715, RIA 485, VKM Ac-965
straininfo link
- @ref: 84258
- straininfo: 45808
literature
- topic: Phylogeny
- Pubmed-ID: 23291893
- title: Streptomyces chilikensis sp. nov., a halophilic streptomycete isolated from brackish water sediment.
- authors: Ray L, Suar M, Pattnaik AK, Raina V
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.046284-0
- year: 2013
- mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Geologic Sediments/*microbiology, India, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/analysis
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
9243 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40061) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40061 | |||
19396 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40061.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
84258 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID45808.1 | StrainInfo: A central database for resolving microbial strain identifiers |