Strain identifier

BacDive ID: 15154

Type strain: Yes

Species: Streptomyces flaveolus

Strain Designation: Actinomyces 168

Variant: Isotype of BacDive ID 15155

Strain history: KCC S-0032 <-- T. Yamaguchi (IAM 0005) <-- MTHU 65 <-- Hyden Chem. Corp. <-- ATCC 3319 <-- IMRU 3319.

NCBI tax ID(s): 67297 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 9243

BacDive-ID: 15154

DSM-Number: 40061

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, amino acid production

description: Streptomyces flaveolus Actinomyces 168 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces amino acids.

NCBI tax id

  • NCBI tax id: 67297
  • Matching level: species

strain history

@refhistory
9243<- E.B. Shirling, ISP <- S.A. Waksman, IMRU
67770KCC S-0032 <-- T. Yamaguchi (IAM 0005) <-- MTHU 65 <-- Hyden Chem. Corp. <-- ATCC 3319 <-- IMRU 3319.

doi: 10.13145/bacdive15154.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces flaveolus
  • full scientific name: Streptomyces flaveolus (Waksman 1923) Waksman and Henrici 1948 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Actinomyces flaveolus

@ref: 9243

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces flaveolus

full scientific name: Streptomyces flaveolus (Waksman 1923) Waksman and Henrici 1948

strain designation: Actinomyces 168

variant: Isotype of BacDive ID 15155

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no90.327
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
19396Yellow10-14 daysISP 2
19396Yellow10-14 daysISP 3
19396Yellow10-14 daysISP 4
19396Yellow10-14 daysISP 5
19396Yellow10-14 daysISP 6
19396Yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19396yesAerial MyceliumGreyISP 2
19396yesAerial MyceliumGreyISP 3
19396yesAerial MyceliumGreyISP 4
19396yesAerial MyceliumGreyISP 5
19396noISP 6
19396yesAerial MyceliumWhiteISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9243GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19396ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19396ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19396ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19396ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19396ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19396ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9243STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf

culture temp

@refgrowthtypetemperaturerange
19396positiveoptimum28mesophilic
9243positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore descriptionspore formationconfidence
19396Formation of spore chains: rectiflixibilis, spore surface: hairyyes
69481yes100
69480yes100

compound production

  • @ref: 9243
  • compound: L isoleucine

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1939662968cellulose+
1939616634raffinose+
1939626546rhamnose+
1939628757fructose+
1939629864mannitol+
1939617268myo-inositol+
1939618222xylose+
1939617992sucrose+
1939622599arabinose+
1939617234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19396+-----++--+

Isolation, sampling and environmental information

isolation

  • @ref: 9243
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
92431Risk group (German classification)
193961Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces flaveolus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4577D44245121ena67297
20218Streptomyces flaveolus gene for 16S rRNA, partial sequence, strain: NBRC 12768AB1841311463ena67297
20218Streptomyces flaveolus gene for 16S rRNA, partial sequence, strain: NBRC 3408AB1847641477ena67297
20218Streptomyces flaveolus gene for 16S rRNA, partial sequence, strain: NBRC 3715AB1847861477ena67297
20218Streptomyces flaveolus strain NRRL B-1334 16S ribosomal RNA gene, partial sequenceEF6540981481ena67297
20218Streptomyces flaveolus strain NRRL B-2688 16S ribosomal RNA gene, partial sequenceAY9997991192ena67297

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces flaveolus JCM 4032GCA_014648815contigncbi67297
66792Streptomyces flaveolus strain JCM 403267297.6wgspatric67297

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes89.882no
anaerobicno99.311no
halophileno93.557no
spore-formingyes96.308no
glucose-utilyes91.393yes
thermophileno98.494no
flagellatedno98.196no
aerobicyes94.548no
glucose-fermentno90.595no
motileno94.547no

External links

@ref: 9243

culture collection no.: DSM 40061, ATCC 19754, ATCC 25460, ATCC 3319, CBS 493.68, CBS 693.69, DSM 40328, ETH 12399, IAM 0117, IFO 12768, IMET 40233, IMRU 3319, ISP 5061, ISP 5328, JCM 4032, JCM 4577, KCC S-0032, KCC S-0577, NBRC 12768, NRRL B-1334, NRRL B-2688, RIA 1035, BCRC 12489, CBS 128.20, CCM 3171, CECT 3181, CGMCC 4.1432, HAMBI 893, ICMP 392, IFO 3408, IFO 3715, KCTC 9022, LMG 19328, NBRC 3408, NBRC 3715, RIA 485, VKM Ac-965

straininfo link

  • @ref: 84258
  • straininfo: 45808

literature

  • topic: Phylogeny
  • Pubmed-ID: 23291893
  • title: Streptomyces chilikensis sp. nov., a halophilic streptomycete isolated from brackish water sediment.
  • authors: Ray L, Suar M, Pattnaik AK, Raina V
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.046284-0
  • year: 2013
  • mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Geologic Sediments/*microbiology, India, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9243Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40061)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40061
19396Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40061.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84258Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45808.1StrainInfo: A central database for resolving microbial strain identifiers