Strain identifier

BacDive ID: 1515

Type strain: Yes

Species: Paucisalibacillus globulus

Strain Designation: B22

Strain history: CIP <- 2005, I. Tiago, Coimbra, Portugal: strain B22

NCBI tax ID(s): 1122940 (strain), 351095 (species)

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General

@ref: 7739

BacDive-ID: 1515

DSM-Number: 18846

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Paucisalibacillus globulus B22 is an aerobe, spore-forming, mesophilic bacterium that was isolated from poting soil.

NCBI tax id

NCBI tax idMatching level
351095species
1122940strain

strain history

@refhistory
7739<- CIP <- I. Tiago; B22
383422005, I. Tiago, Coimbra, Portugal: strain B22
119649CIP <- 2005, I. Tiago, Coimbra, Portugal: strain B22

doi: 10.13145/bacdive1515.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Paucisalibacillus
  • species: Paucisalibacillus globulus
  • full scientific name: Paucisalibacillus globulus Nunes et al. 2006

@ref: 7739

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Paucisalibacillus

species: Paucisalibacillus globulus

full scientific name: Paucisalibacillus globulus Nunes et al. 2006

strain designation: B22

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31781positive03-07 µm0.5 µmrod-shapedyes
69480yes98.189
69480positive100
119649positiverod-shapedyes

colony morphology

  • @ref: 119649
  • hemolysis ability: 1

pigmentation

  • @ref: 31781
  • production: yes

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_18846_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_18846_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_18846_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_18846_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_18846_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7739BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
38342MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119649CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
119649CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
7739positivegrowth30mesophilic
31781positivegrowth20-45
31781positiveoptimum37mesophilic
38342positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
31781positivegrowth6-9.5alkaliphile
31781positiveoptimum8-8.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31781aerobe
119649obligate aerobe

spore formation

@refspore formationconfidence
31781yes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
31781NaClpositivegrowth0-8 %
31781NaClpositiveoptimum1 %

observation

  • @ref: 31781
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3178130089acetate+carbon source
3178128757fructose+carbon source
3178133984fucose+carbon source
3178128260galactose+carbon source
3178117234glucose+carbon source
3178129987glutamate+carbon source
3178117716lactose+carbon source
3178125115malate+carbon source
3178117306maltose+carbon source
3178129864mannitol+carbon source
3178137684mannose+carbon source
3178115361pyruvate+carbon source
3178116634raffinose+carbon source
3178126546rhamnose+carbon source
3178117822serine+carbon source
3178117992sucrose+carbon source
3178126986threonine+carbon source
3178127082trehalose+carbon source
3178117151xylitol+carbon source
317814853esculin+hydrolysis
11964917632nitrate+reduction

metabolite production

  • @ref: 119649
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31781catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
119649oxidase-
119649alcohol dehydrogenase-1.1.1.1
119649catalase+1.11.1.6
119649lysine decarboxylase-4.1.1.18
119649ornithine decarboxylase-4.1.1.17
119649urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119649-+---+---+++---+----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
7739poting soilPortugalPRTEurope
119649Environment, Poting SoilPortugalPRTEurope2003

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
77391Risk group (German classification)
1196491Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7739
  • description: Paucisalibacillus globulus partial 16S rRNA gene, type strain B22T
  • accession: AM114102
  • length: 1547
  • database: ena
  • NCBI tax ID: 351095

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paucisalibacillus globulus DSM 18846GCA_000482485scaffoldncbi1122940
66792Paucisalibacillus globulus DSM 188461122940.3wgspatric1122940
66792Paucisalibacillus globulus DSM 188462528768179draftimg1122940

GC content

  • @ref: 7739
  • GC-content: 37.9

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes89.072yes
flagellatedyes79.141no
gram-positiveyes88.03yes
anaerobicno98.908no
aerobicyes88.111no
halophileyes74.487yes
spore-formingyes94.943no
thermophileno98.379yes
glucose-utilyes85.811yes
glucose-fermentno83.056no

External links

@ref: 7739

culture collection no.: DSM 18846, CIP 108857, LMG 23148

straininfo link

  • @ref: 71161
  • straininfo: 264632

literature

  • topic: Phylogeny
  • Pubmed-ID: 16902018
  • title: Paucisalibacillus globulus gen. nov., sp. nov., a Gram-positive bacterium isolated from potting soil.
  • authors: Nunes I, Tiago I, Pires AL, da Costa MS, Verissimo A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64261-0
  • year: 2006
  • mesh: Bacillaceae/chemistry/*classification/isolation & purification/physiology, Base Sequence, Culture Media, Fatty Acids/analysis, Molecular Sequence Data, Peptidoglycan/analysis, Phylogeny, Portugal, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sodium Chloride, *Soil Microbiology, Spores, Bacterial, Vitamin K 2/analysis
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
7739Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18846)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18846
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31781Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2805328776041
38342Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6533
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life28604660 35: 676-683 2017
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71161Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID264632.1StrainInfo: A central database for resolving microbial strain identifiers
119649Curators of the CIPCollection of Institut Pasteur (CIP 108857)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108857