Strain identifier

BacDive ID: 15132

Type strain: No

Species: Streptomyces phaeochromogenes

Strain Designation: FH 1277

Strain history: KCC S-0958 <-- DSM 40741 <-- Farbwerke Hoechst AG, FH 1277.

NCBI tax ID(s): 1923 (species)

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General

@ref: 9760

BacDive-ID: 15132

DSM-Number: 40741

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, antibiotic compound production

description: Streptomyces phaeochromogenes FH 1277 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 1923
  • Matching level: species

strain history

@refhistory
9760<- Hoechst AG, FH 1277
67770KCC S-0958 <-- DSM 40741 <-- Farbwerke Hoechst AG, FH 1277.

doi: 10.13145/bacdive15132.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces phaeochromogenes
  • full scientific name: Streptomyces phaeochromogenes (Conn 1917) Waksman 1957 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Actinomyces pheochromogenus
    20215Streptomyces ederensis

@ref: 9760

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces phaeochromogenes

full scientific name: Streptomyces phaeochromogenes (Conn 1917) Waksman 1957

strain designation: FH 1277

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

@refcolony colorincubation periodmedium used
18665Brown10-14 daysISP 2
18665Brown10-14 daysISP 3
18665Brown10-14 daysISP 4
18665Dark black/brown10-14 daysISP 5
18665Brown10-14 daysISP 6
18665Brown10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18665yesAerial MyceliumGrayISP 2
18665yesAerial MyceliumGrayISP 3
18665yesAerial MyceliumGrayISP 4
18665yesAerial MyceliumWhiteISP 5
18665noISP 6
18665yesAerial MyceliumGrayISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9760GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18665ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18665ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18665ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18665ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18665ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18665ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
18665positiveoptimum28mesophilic
9760positivegrowth37mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore descriptionspore formationconfidence
18665Formation of spore chains (rectiflixibilis), spore surface smoothyes
69481yes100
69480yes100

compound production

@refcompound
9760moenomycin
67770Moenomycins A, B1, B2 and C

halophily

  • @ref: 18665
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1866517234glucose+
1866522599arabinose+/-
1866517992sucrose+
1866518222xylose+/-
1866517268myo-inositol+
1866529864mannitol+
1866528757fructose+
1866526546rhamnose+
1866516634raffinose+
1866562968cellulose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6777028908bambermycinyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18665++----++-++

Isolation, sampling and environmental information

isolation

@refsample type
9760soil
67770Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
186651German classification
97601Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces ederensis gene for 16S rRNA, partial sequenceAB018209120ena83383
20218Streptomyces ederensis strain KCTC 9726 16S ribosomal RNA gene, partial sequenceAY9998241420ena83383
20218Streptomyces ederensis gene for 16S rRNA, partial sequence, strain: NBRC 15410AB1846581483ena83383
20218Streptomyces ederensis strain NRRL B-8146 16S ribosomal RNA gene, partial sequenceEU5944811490ena83383

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces ederensis strain JCM 495883383.3wgspatric67370
66792Streptomyces ederensis CECT 32122863240559draftimg83383
67770Streptomyces umbrinus JCM 4958GCA_014656275scaffoldncbi67370
66792Streptomyces umbrinus CECT 3212GCA_024648905scaffoldncbi67370

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno93.382no
flagellatedno97.655no
gram-positiveyes87.441no
anaerobicno99.339no
halophileno94.153no
spore-formingyes94.167yes
thermophileno99.171yes
glucose-utilyes91.19no
aerobicyes93.045no
glucose-fermentno88.501no

External links

@ref: 9760

culture collection no.: DSM 40741, ATCC 15304, JCM 4958, BCRC 11896, CBS 545.70, CECT 3212, CGMCC 4.1665, IFO 15410, KCTC 9726, NBRC 15410, NRRL B-8146, VKM Ac-845

straininfo link

  • @ref: 84237
  • straininfo: 265445

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28382471Two new species of the genus Streptomyces: Streptomyces camponoti sp. nov. and Streptomyces cuticulae sp. nov. isolated from the cuticle of Camponotus japonicus Mayr.Piao C, Zheng W, Li Y, Liu C, Jin L, Song W, Yan K, Wang X, Xiang WArch Microbiol10.1007/s00203-017-1353-62017Animals, Ants/*microbiology, Bacterial Typing Techniques, China, DNA Gyrase/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces/classification/genetics/isolation & purificationTranscriptome
Phylogeny35471105Streptomyces liliifuscus sp. nov and an anti-ginger plague agent Streptomyces liliiviolaceus sp. nov, two novel species isolated from soil of Lilium lancifolium.Li K, Man Y, Liu J, Liu Z, Ma H, Zhu H, Zhou Y, Zhang C, Zhou XInt J Syst Evol Microbiol10.1099/ijsem.0.0053402022Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Ginger/genetics, *Lilium/genetics, Phylogeny, *Plague, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, Soil Microbiology, *StreptomycesTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9760Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40741)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40741
18665Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40741.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84237Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265445.1StrainInfo: A central database for resolving microbial strain identifiers